Yeast Genetic Engineering PDF Download
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| Author | : Henri Heslot |
| Publisher | : CRC Press |
| Total Pages | : 818 |
| Release | : 2018-01-18 |
| Genre | : Technology & Engineering |
| ISBN | : 1351091638 |
Download Molecular Biology and Genetic Engineering of Yeasts Book in PDF, ePub and Kindle
Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.
| Author | : Philip J. Barr |
| Publisher | : Butterworth-Heinemann |
| Total Pages | : 384 |
| Release | : 1989 |
| Genre | : Science |
| ISBN | : |
Download Yeast Genetic Engineering Book in PDF, ePub and Kindle
An illustration of the impressive development of yeast gene expression systems accomplished in recent years which demonstrates the power of these systems for the production of proteins in quantities useful as therapeutics, industrial enzymes and research agents.
| Author | : Valeria Mapelli |
| Publisher | : Humana Press |
| Total Pages | : 0 |
| Release | : 2014-04-18 |
| Genre | : Science |
| ISBN | : 9781493905621 |
Download Yeast Metabolic Engineering Book in PDF, ePub and Kindle
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
| Author | : Valeria Mapelli |
| Publisher | : Springer Nature |
| Total Pages | : 300 |
| Release | : 2022-07-04 |
| Genre | : Science |
| ISBN | : 1071623990 |
Download Yeast Metabolic Engineering Book in PDF, ePub and Kindle
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
| Author | : Antonio Morata |
| Publisher | : BoD – Books on Demand |
| Total Pages | : 298 |
| Release | : 2019-09-04 |
| Genre | : Technology & Engineering |
| ISBN | : 1789846129 |
Download Advances in Grape and Wine Biotechnology Book in PDF, ePub and Kindle
Advances in Grape and Wine Biotechnology is a collection of fifteen chapters that addresses different issues related to the technological and biotechnological management of vineyards and winemaking. It focuses on recent advances in the field of viticulture with interesting topics such as the development of a microvine model for research purposes, the mechanisms of cultivar adaptation and evolution in a climate change scenario, and the consequences of vine water deficit on yield components. Other topics include the metabolic profiling of different Saccharomyces and non-Saccharomyces yeast species and their contribution in modulating the sensory quality of wines produced in warm regions, the use of new natural and sustainable fining agents, and available physical methods to reduce alcohol content. This volume will be of great interest to researchers and vine or wine professionals.
| Author | : Philip J. Barr |
| Publisher | : |
| Total Pages | : 354 |
| Release | : 1986 |
| Genre | : |
| ISBN | : |
Download Yeast Genetic Engineering Book in PDF, ePub and Kindle
| Author | : John F.T. Spencer |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 110 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642733565 |
Download Yeast Genetics Book in PDF, ePub and Kindle
The manual consists of two main sections. The first includes the essential, sometimes laborious, procedures for handling yeasts, for inducing mating and isolation of hybrids, for inducing sporulation and isolation of single-spore clones, with some details of tetrad analysis, and including techniques and ancillary equipment for use of the micromanipulator. There are also proce dures for induction of mutants by physical and chemical agents, and for iso lation of particular types of mutants, such as to temperature sensitivity, for increased frequency of mutations, for mutations in the mitochondrial genome, both to the petite colonie form and to resistance to antibiotics, for mutations in that part of the yeast genome controlling the glycolytic cycle, and numerous others. Mapping of mutations is discussed briefly, though this aspect of yeast genetics is probably one which should not be undertaken until the investigator has gained a certain amount of experience in the field. How ever, as is pointed out in the pertinent part of the manual, the task of mapping has been tremendouslysimplified by the availability from the Yeast Genetics Stock Center at the University of California at Berkeley of a set of auxo trophic strains designed to permit mapping of most unknown genes with a minimum number of crosses and tetrad analyses. The first section concludes with the description of methods for hybridization of yeasts by protoplast fusion, which has been described as the poor man's system for genetic engineering.
| Author | : J.F.T. Spencer |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 546 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461254914 |
Download Yeast Genetics Book in PDF, ePub and Kindle
During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. There great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspectiv(}-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily.
| Author | : Alan Wiseman |
| Publisher | : Prentice Hall |
| Total Pages | : 216 |
| Release | : 1991 |
| Genre | : Science |
| ISBN | : |
Download Genetically-engineered Proteins and Enzymes from Yeasts Book in PDF, ePub and Kindle
Presents the important concepts and advances on how to successfully use genetic engineering techniques to obtain human proteins and enzymes - an area of vital importance for the production of human proteins for medical purposes.
| Author | : T. Satyanarayana |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 747 |
| Release | : 2009-04-24 |
| Genre | : Science |
| ISBN | : 1402082924 |
Download Yeast Biotechnology: Diversity and Applications Book in PDF, ePub and Kindle
I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 F ormer Vice-chancellor, JNU Director, NCPGR (New Delhi) Pr eface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the na- ral environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, a- mals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transfor- tion using recombinant technology. Yeasts (e. g.
