Yeast Cell Surface Engineering PDF Download
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| Author | : Mitsuyoshi Ueda |
| Publisher | : Springer |
| Total Pages | : 185 |
| Release | : 2019-04-23 |
| Genre | : Medical |
| ISBN | : 9811358680 |
Download Yeast Cell Surface Engineering Book in PDF, ePub and Kindle
This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.
| Author | : Rawil Fakhrullin |
| Publisher | : Royal Society of Chemistry |
| Total Pages | : 266 |
| Release | : 2014-07-24 |
| Genre | : Medical |
| ISBN | : 1849739021 |
Download Cell Surface Engineering Book in PDF, ePub and Kindle
A summary of the recent achievements in surface-functionalised cells including fabrication, characterisation, applications and nanotoxicity.
| Author | : Bin Liu |
| Publisher | : Humana |
| Total Pages | : 0 |
| Release | : 2015-06-11 |
| Genre | : Science |
| ISBN | : 9781493927470 |
Download Yeast Surface Display Book in PDF, ePub and Kindle
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
| Author | : Michael W. Traxlmayr |
| Publisher | : Springer Nature |
| Total Pages | : 643 |
| Release | : 2022-04-28 |
| Genre | : Science |
| ISBN | : 1071622854 |
Download Yeast Surface Display Book in PDF, ePub and Kindle
This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
| Author | : Rawil F Fakhrullin |
| Publisher | : Royal Society of Chemistry |
| Total Pages | : 267 |
| Release | : 2014-07-08 |
| Genre | : Technology & Engineering |
| ISBN | : 1782628479 |
Download Cell Surface Engineering Book in PDF, ePub and Kindle
Cell surface engineering is an emerging field concerning cell surface modifications to enhance its functionalities. The book introduces the reader to the area of surface-functionalized cells and summarizes recent developments in the area including fabrication, characterization, applications and nanotoxicity. Topics covered include recent approaches for the functionalization of cells with nanomaterials (polymer nanofilms and nanoparticles), fabrication of functional biomimetic devices and assemblies based on nanoparticle-modified microbial cells and artificial spores (the bioinspired encapsulation of living cells with inorganic nanoshells). The book provides an interdisciplinary approach to the topic with authors from both biological and chemical backgrounds. This multidisciplinary view makes the book suitable for those interested in biomaterials, biochemistry, microbiology and colloid chemistry, providing both an introduction for postgraduate students as well as a comprehensive summary for those already working in the area. biomaterials, biochemistry, microbiology and colloid chemistry.
| Author | : Bin Liu |
| Publisher | : Humana |
| Total Pages | : 0 |
| Release | : 2016-10-09 |
| Genre | : Science |
| ISBN | : 9781493946679 |
Download Yeast Surface Display Book in PDF, ePub and Kindle
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
| Author | : Anne Zijing Ye |
| Publisher | : |
| Total Pages | : |
| Release | : 2019 |
| Genre | : |
| ISBN | : |
Download Engineering an Improved Bioconjugation Enzyme Book in PDF, ePub and Kindle
Enzyme engineering is a valuable tool for generating powerful and robust biocatalysts for a broad range of applications. Directed evolution using cell surface display has been previously applied for enzyme engineering, and involves iterative optimization of a protein sequence to yield a desired phenotype. However, previous applications have been carefully tailored for specific enzymes, and this process of developing a good screen is a significant bottleneck in engineering. Co-display of an enzyme and its substrate on the yeast surface is a promising general strategy for evolving bond-forming enzymes, but has only been used to engineer one enzyme. We extended yeast surface co-display to engineer Sfp, a bioconjugation enzyme, for improved activity with a non-native substrate. Sfp library screening identified mutational hotspots in the enzyme and led to the design of Sfp variants with orders of magnitude enhancements in activity. However, these activity improvements were only apparent in contexts similar to yeast surface co-display, reflecting artifacts in the use of this approach for enzyme engineering. Based on our results, we offer suggestions for improving the success of engineering strategies based on yeast surface display.
| Author | : Valeria Mapelli |
| Publisher | : Springer Nature |
| Total Pages | : 300 |
| Release | : 2022-07-04 |
| Genre | : Science |
| ISBN | : 1071623990 |
Download Yeast Metabolic Engineering Book in PDF, ePub and Kindle
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
| Author | : Huimin Zhao |
| Publisher | : John Wiley & Sons |
| Total Pages | : 41 |
| Release | : 2021-08-23 |
| Genre | : Science |
| ISBN | : 3527344705 |
Download Protein Engineering Book in PDF, ePub and Kindle
A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.
| Author | : Management Association, Information Resources |
| Publisher | : IGI Global |
| Total Pages | : 1837 |
| Release | : 2017-01-11 |
| Genre | : Technology & Engineering |
| ISBN | : 1522517995 |
Download Materials Science and Engineering: Concepts, Methodologies, Tools, and Applications Book in PDF, ePub and Kindle
The design and study of materials is a pivotal component to new discoveries in the various fields of science and technology. By better understanding the components and structures of materials, researchers can increase its applications across different industries. Materials Science and Engineering: Concepts, Methodologies, Tools, and Applications is a compendium of the latest academic material on investigations, technologies, and techniques pertaining to analyzing the synthesis and design of new materials. Through its broad and extensive coverage on a variety of crucial topics, such as nanomaterials, biomaterials, and relevant computational methods, this multi-volume work is an essential reference source for engineers, academics, researchers, students, professionals, and practitioners seeking innovative perspectives in the field of materials science and engineering.
