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Viral Applications of Green Fluorescent Protein

Viral Applications of Green Fluorescent Protein
Author: Barry W. Hicks
Publisher: Humana Press
Total Pages: 384
Release: 2009-04-01
Genre: Science
ISBN: 9781934115879

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Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.


Green Fluorescent Protein

Green Fluorescent Protein
Author: Martin Chalfie
Publisher: Wiley-Liss
Total Pages: 424
Release: 1998-09-07
Genre: Science
ISBN:

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Green Fluorescent Protein: Properties, Applications, and Protocols is the first book devoted to this rapidly evolving reporter system. This collection of essays from pioneers in the field tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. The book clearly demonstrates how to tailor GFP to specific systems, maximize expression and enhance detection in a variety of organisms and cell types, generate variants with altered properties, explore new ways to use GFP, and more. Topics covered include: * The discovery of GFP * Bioluminescence and biofluorescence in nature * Biochemical and physical properties of GFP * The three-dimensional structure of GFP and its implications for function and design * Molecular biology and modification of GFP * Uses of GFP in various organisms, including prokaryotes, yeast, C. elegans, drosophila, plants, transgenic vertebrates, and mammalian cells * Detailed protocols for the expression of GFP, specimen preparation, visualization and recording of GFP fluorescence, and GFP websites


Fluorescent Proteins II

Fluorescent Proteins II
Author: Gregor Jung
Publisher: Springer Science & Business Media
Total Pages: 287
Release: 2012-01-05
Genre: Science
ISBN: 3642233767

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Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.


Green Fluorescent Protein

Green Fluorescent Protein
Author: Barry W. Hicks
Publisher: Humana Press
Total Pages: 394
Release: 2002-06-20
Genre: Science
ISBN: 9780896039056

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Could there be a better time to be a life scientist? In the past two decades, a host of new techniques have been added to the tool chests of biochemists and molecular biologists. A wonderful benefit of the basic scientific research that fueled the advances in these fields is the wide variety of direct applications in agriculture and medicine. Even with all of these advances, and with the accompanying explosion in computer and information technology, it is clear that the depth of our ignorance vastly exceeds the breadth of our knowledge about complex organisms at the molecular level. Any new techniques or materials that allow us to extend our research-based knowledge should be w- comed and utilized to their fullest potentials. With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented. Although the text has chapters that emphasize different areas of s- cialization, it is not meant to send molecular biologists to one section, botanists to another, and clinicians to still another. Perhaps the most valuable exchange for people in any discipline will come from seeing how others have been able to apply GFP in fields outside of their immediate areas of expertise. GFP from Aequorea victoria is a fluorescent marker protein, and there are certainly other useful fluorophore markers.


Illuminating Disease

Illuminating Disease
Author: Marc Zimmer
Publisher: Oxford University Press
Total Pages: 241
Release: 2015-01-05
Genre: Science
ISBN: 0199362831

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Green fluorescent proteins have been floating in the ocean for more than 160 million years, but it took a curious scientist, fascinated by pinpricks of green light, to begin unlocking their potential. Now these jellyfish proteins have become one of the most important tools available to researchers in modern medicine and biology. By using them to illuminate other proteins that were previously invisible even under a microscope, scientists are now able to observe facets of disease that would have otherwise gone undetected. Green fluorescent proteins are used in over three million experiments a year and have proved invaluable for tasks such as tracking HIV, breeding bird flu-resistant chickens, and confirming the existence of cancerous stem cells. In Illuminating Disease, Marc Zimmer introduces us to these revolutionary proteins, acquainting readers both with the researchers responsible for the proteins' discovery as well as their wide utility. The book details the history of genetically modified fluorescent parasites and viruses, which provide scientists with lifesaving information about the spread of diseases. Green fluorescent proteins allow scientists and doctors to understand diseases better by quite literally illuminating various microscopic interactions occurring in living cells that otherwise would have gone unseen. The book is richly illustrated, showing the visually striking uses of green fluorescent proteins, and many of these scans have won awards in biological imaging competitions. An ideal introduction for students and advanced researchers alike, Illuminating Disease is an accessible yet deeply probing investigation into one of the most important developments in medical research of the last several decades.


Viral Applications of Green Fluorescent Protein

Viral Applications of Green Fluorescent Protein
Author: Barry W. Hicks
Publisher: Humana Press
Total Pages: 315
Release: 2009-04-01
Genre: Science
ISBN: 9781934115879

Download Viral Applications of Green Fluorescent Protein Book in PDF, ePub and Kindle

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.


Plant Virology

Plant Virology
Author: Adrian Gibbs
Publisher: Hodder Education
Total Pages: 292
Release: 1980
Genre: Plant viruses
ISBN: 9780713127645

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The history and scope of plant virology; Some plant viruses and their names; Effects of viruses on plants; Experimental transmission; The composition and structure of the particles of plant viruses; The purification of virus particles, and some properties of purifield preparations; Infectivity assay; Serological methods; Physical and chemical methods of assay and analysis; The effects of inactivators on virus particles; Behaviour of viruses in plants; Variation, strains and classification; Transmission by vectors and in other natural ways; Virus ecology; Ways of preventing crop losses; Viruses of organisms other than higher plants; Origins of viruses; Plant pathogens confused with viruses.


A Guide to Green-fluorescent Protein

A Guide to Green-fluorescent Protein
Author: Catherine M. Thomson
Publisher: Drug & Market Development Publications
Total Pages:
Release: 2003-12-30
Genre: Medical
ISBN: 9781579362782

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Viral Structural Proteins: Advances in Research and Application: 2011 Edition

Viral Structural Proteins: Advances in Research and Application: 2011 Edition
Author:
Publisher: ScholarlyEditions
Total Pages: 34
Release: 2012-01-09
Genre: Medical
ISBN: 1464951403

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Viral Structural Proteins: Advances in Research and Application: 2011 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Viral Structural Proteins in a compact format. The editors have built Viral Structural Proteins: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Viral Structural Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Viral Structural Proteins: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.