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| Author | : Paul N. MacDonald |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 337 |
| Release | : 2008-02-03 |
| Genre | : Science |
| ISBN | : 1592592104 |
Download Two-Hybrid Systems Book in PDF, ePub and Kindle
The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
| Author | : Paul L. Bartel |
| Publisher | : Oxford University Press, USA |
| Total Pages | : 362 |
| Release | : 1997 |
| Genre | : Carrier proteins |
| ISBN | : 9780195109382 |
Download The Yeast Two-hybrid System Book in PDF, ePub and Kindle
This volume, part of the Advances in Molecular Biology series, presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it illustrates how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique.
| Author | : Gabriel Waksman |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 325 |
| Release | : 2006-12-22 |
| Genre | : Medical |
| ISBN | : 0387245324 |
Download Proteomics and Protein-Protein Interactions Book in PDF, ePub and Kindle
Gabriel Waksman Institute of Structural Molecular Biology, Birkbeck and University College London, Malet Street, London WC1E 7HX, United Kingdom Address for correspondence: Professor Gabriel Waksman Institute of Structural Molecular Biology Birkbeck and University College London Malet Street London WC1E 7H United Kingdom Email: g. waksman@bbk. ac. uk and g. waksman@ucl. ac. uk Phone: (+44) (0) 207 631 6833 Fax: (+44) (0) 207 631 6833 URL: http://people. cryst. bbk. ac. uk/?ubcg54a Gabriel Waksman is Professor of Structural Molecular Biology at the Institute of Structural Molecular Biology at UCL/Birkbeck, of which he is also the director. Before joining the faculty of UCL and Birkbeck, he was the Roy and Diana Vagelos Professor of Biochemistry and Molecular Biophysics at the Washington University School of Medicine in St Louis (USA). The rapidly evolving ?eld of protein science has now come to realize the ubiquity and importance of protein–protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of hi- throughput proteomics to monitor protein–protein interactions at an organism level, we can now safely state that protein–protein interactions are the norm and not the exception.
| Author | : Weibo Cai |
| Publisher | : BoD – Books on Demand |
| Total Pages | : 488 |
| Release | : 2012-03-30 |
| Genre | : Science |
| ISBN | : 9535103970 |
Download Protein-Protein Interactions Book in PDF, ePub and Kindle
Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.
| Author | : Arjan J. van der Schaft |
| Publisher | : Springer |
| Total Pages | : 189 |
| Release | : 2007-10-03 |
| Genre | : Technology & Engineering |
| ISBN | : 1846285429 |
Download An Introduction to Hybrid Dynamical Systems Book in PDF, ePub and Kindle
This book is about dynamical systems that are "hybrid" in the sense that they contain both continuous and discrete state variables. Recently there has been increased research interest in the study of the interaction between discrete and continuous dynamics. The present volume provides a first attempt in book form to bring together concepts and methods dealing with hybrid systems from various areas, and to look at these from a unified perspective. The authors have chosen a mode of exposition that is largely based on illustrative examples rather than on the abstract theorem-proof format because the systematic study of hybrid systems is still in its infancy. The examples are taken from many different application areas, ranging from power converters to communication protocols and from chaos to mathematical finance. Subjects covered include the following: definition of hybrid systems; description formats; existence and uniqueness of solutions; special subclasses (variable-structure systems, complementarity systems); reachability and verification; stability and stabilizability; control design methods. The book will be of interest to scientists from a wide range of disciplines including: computer science, control theory, dynamical system theory, systems modeling and simulation, and operations research.
| Author | : Ricardo G. Sanfelice |
| Publisher | : Princeton University Press |
| Total Pages | : 420 |
| Release | : 2021-01-12 |
| Genre | : Mathematics |
| ISBN | : 0691180229 |
Download Hybrid Feedback Control Book in PDF, ePub and Kindle
"Hybrid systems are those that-unlike classical systems-exhibit both discrete changes, or "jumps", and continuous changes, or "flow." The canonical example of a hybrid system is a bouncing ball: the ball's speed changes continuously between bounces, but there is a discrete jump in velocity each time the ball impacts the ground. Hybrid systems feature widely across disciplines, including in biology, computer science, and mechanical engineering; examples range from fireflies to self-driving cars. Although classical control theory provides powerful tools for analyzing systems that exhibit either flow or jumps, it is ill-equipped to handle hybrid systems, which feature both behaviors. In Hybrid Feedback Control, Ricardo Sanfelice presents a self-contained introduction to the control of hybrid systems, and develops new tools for their design and analysis. This monograph uses hybrid systems notation to present a new, unified control theory framework, thus filling an important gap in the control theory literature. In addition to presenting this theoretical framework, the book also includes a variety of examples and exercises, a Matlab toolbox, and a summary at the beginning of each chapter. The book was originally used in a series of lectures on the topic, and will find a modest amount of crossover course use. The book will also find use outside the field of control, particularly in dynamical systems theory, applied mathematics, and computer science"--
| Author | : Wassim M. Haddad |
| Publisher | : Princeton University Press |
| Total Pages | : 522 |
| Release | : 2014-09-08 |
| Genre | : Mathematics |
| ISBN | : 1400865247 |
Download Impulsive and Hybrid Dynamical Systems Book in PDF, ePub and Kindle
This book develops a general analysis and synthesis framework for impulsive and hybrid dynamical systems. Such a framework is imperative for modern complex engineering systems that involve interacting continuous-time and discrete-time dynamics with multiple modes of operation that place stringent demands on controller design and require implementation of increasing complexity--whether advanced high-performance tactical fighter aircraft and space vehicles, variable-cycle gas turbine engines, or air and ground transportation systems. Impulsive and Hybrid Dynamical Systems goes beyond similar treatments by developing invariant set stability theorems, partial stability, Lagrange stability, boundedness, ultimate boundedness, dissipativity theory, vector dissipativity theory, energy-based hybrid control, optimal control, disturbance rejection control, and robust control for nonlinear impulsive and hybrid dynamical systems. A major contribution to mathematical system theory and control system theory, this book is written from a system-theoretic point of view with the highest standards of exposition and rigor. It is intended for graduate students, researchers, and practitioners of engineering and applied mathematics as well as computer scientists, physicists, and other scientists who seek a fundamental understanding of the rich dynamical behavior of impulsive and hybrid dynamical systems.
| Author | : Dev Kambhampati |
| Publisher | : John Wiley & Sons |
| Total Pages | : 276 |
| Release | : 2006-03-06 |
| Genre | : Science |
| ISBN | : 3527605215 |
Download Protein Microarray Technology Book in PDF, ePub and Kindle
This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins. The list of authors consisting of world leading experts provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions. Topics covered include: -Types of biomolecular interactions -Surface chemistry -Detection technologies -Spotting technologies -Bioinformatics/data analysis. While primarily intended to serve as a reference for researchers and students embarking on the exciting fields of proteomics, drug discovery and clinical diagnostics, this technology is also expected to potentially impact the areas of food diagnostics, environmental monitoring and national security.
| Author | : Rafal Goebel |
| Publisher | : Princeton University Press |
| Total Pages | : 227 |
| Release | : 2012-03-18 |
| Genre | : Mathematics |
| ISBN | : 1400842638 |
Download Hybrid Dynamical Systems Book in PDF, ePub and Kindle
Hybrid dynamical systems exhibit continuous and instantaneous changes, having features of continuous-time and discrete-time dynamical systems. Filled with a wealth of examples to illustrate concepts, this book presents a complete theory of robust asymptotic stability for hybrid dynamical systems that is applicable to the design of hybrid control algorithms--algorithms that feature logic, timers, or combinations of digital and analog components. With the tools of modern mathematical analysis, Hybrid Dynamical Systems unifies and generalizes earlier developments in continuous-time and discrete-time nonlinear systems. It presents hybrid system versions of the necessary and sufficient Lyapunov conditions for asymptotic stability, invariance principles, and approximation techniques, and examines the robustness of asymptotic stability, motivated by the goal of designing robust hybrid control algorithms. This self-contained and classroom-tested book requires standard background in mathematical analysis and differential equations or nonlinear systems. It will interest graduate students in engineering as well as students and researchers in control, computer science, and mathematics.
| Author | : Massimiliano Lo Faro |
| Publisher | : Academic Press |
| Total Pages | : 530 |
| Release | : 2021-10-30 |
| Genre | : Science |
| ISBN | : 0128241861 |
Download Hybrid Technologies for Power Generation Book in PDF, ePub and Kindle
Hybrid Technologies for Power Generation addresses the topics related to hybrid technologies by coupling conventional thermal engines with novel technologies, including fuel cells, batteries, thermal storage and electrolysis, and reporting on the most recent advances concerning transport and stationary applications. Potential operating schemes of hybrid power generation systems are covered, highlighting possible combinations of technology and guideline selection according to the energy demands of end-users. Going beyond state-of-the-art technological developments for processes, devices and systems, this book discusses the environmental impact and existing hurdles of moving from a single device to new approaches for efficient energy generation, transfer, conversion, high-density storage and consumption. By describing the practical viability of novel devices coupled to conventional thermal devices, this book has a decisive impact in energy system research, supporting those in the energy research and engineering communities. Covers detailed thermodynamic requirements for multiple smart technologies included in hybrid systems (i.e., FC, electrolysers, supercapacitors, batteries, thermal storage, etc.) Features fundamental analysis and modeling to optimize the combination of smart technologies with traditional engines Details protocols for the analysis, operation and requirements of large-scale production
