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It is known that the LHC has a considerable discovery potential because of its large centre-of-mass energy (vs =14 TeV) and the high design luminosity. In addition, the two experiments ATLAS and CMS perform precision measurements for numerous models in physics. The increasing experimental precision demands an even higher level of accuracy on the theoretical side. For a more precise prediction of outcomes, one has to consider the corrections obtained typically from Quantum Chromodynamics (QCD). The calculation of these corrections in the high energy regime is described by perturbation theory. In the present study, multi-loop calculations in QCD, including in particular two-loop corrections for single top quark production, are considered. There are several phenomenological motivations to study single top quark production: Firstly, the process is sensitive to the electroweak Wtb-vertex; moreover, non-standard couplings can hint at physics beyond the Standard Model. Secondly, the t-channel cross section measurement provides information on the b-quark Parton Distribution Functions (PDF). Finally, single top quark production enables us to directly measure the Cabibbo-Kobayashi-Maskawa(CKM) matrix element Vtb. The next-to-next-to-leading-order (NNLO) calculation of the single top quark production has many building blocks. In this study, two blocks will be presented: one-loop corrections squared and two-loop corrections interfered with Born. Initially, the one-loop squared contribution at NNLO for single top quark production will be calculated. Before we begin with the calculation of the two-loop corrections to single top quark production, we calculate the QCD form factors of heavy quarks at NNLO, along with the axial vector coupling as a first independent check. A comparison with the relevant literature suggests that this approach is in line with generally accepted procedure. This consistency check provides a proof of the validity of our setup. In the next step, the two-loop corrections to single top quark production will be calculated. After reducing all occurring tensor integrals to scalar integrals, we apply the integration by parts method (IBP) to find the master integrals. This step is a major challenge compared to all similar calculations because of the number of variables in the problem (two Mandelstam variables s and t, the dimension d and the mass of the top quark mt as well as the mass of the W boson mw). Finally, the calculation of the three kinds of topologies – vertex corrections, double boxes and non-planar double boxes – in the two-loop contribution at NNLO calculation will be presented.