Three Dimensional Electron Microscopy Of Macromolecular Assemblies PDF Download
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| Author | : Joachim Frank |
| Publisher | : Oxford University Press |
| Total Pages | : 427 |
| Release | : 2006 |
| Genre | : Science |
| ISBN | : 0195150961 |
Download Three-dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Cryoelectron microscopy of biological molecules is the growth area in biophysics and structural biology. A number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
| Author | : Joachim Frank |
| Publisher | : |
| Total Pages | : 342 |
| Release | : 1996-01-01 |
| Genre | : Science |
| ISBN | : 9780122650406 |
Download Three-dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel; * the ribosome * chaperonins
| Author | : Frank Joachim |
| Publisher | : Elsevier |
| Total Pages | : 342 |
| Release | : 1996-01-24 |
| Genre | : Science |
| ISBN | : 9780080525815 |
Download Three-Dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel; * the ribosome * chaperonins
| Author | : Joachim Frank |
| Publisher | : Oxford University Press |
| Total Pages | : 432 |
| Release | : 2006-02-02 |
| Genre | : Science |
| ISBN | : 9780198034384 |
Download Three-Dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
| Author | : Joachim Frank |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 398 |
| Release | : 2013-04-17 |
| Genre | : Science |
| ISBN | : 1475721633 |
Download Electron Tomography Book in PDF, ePub and Kindle
This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.
| Author | : Roland A. Fleck |
| Publisher | : John Wiley & Sons |
| Total Pages | : 741 |
| Release | : 2019-04-29 |
| Genre | : Science |
| ISBN | : 1118654064 |
Download Biological Field Emission Scanning Electron Microscopy, 2 Volume Set Book in PDF, ePub and Kindle
The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.
| Author | : Maria Armenia Carrondo |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 213 |
| Release | : 2011-12-01 |
| Genre | : Science |
| ISBN | : 9400725299 |
Download Macromolecular Crystallography Book in PDF, ePub and Kindle
This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics. A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.
| Author | : Arvid B. Maunsbach |
| Publisher | : Elsevier |
| Total Pages | : 548 |
| Release | : 1998-11-03 |
| Genre | : Science |
| ISBN | : 9780080528090 |
Download Biomedical Electron Microscopy Book in PDF, ePub and Kindle
This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs
| Author | : J. Richard McIntosh |
| Publisher | : Elsevier |
| Total Pages | : 878 |
| Release | : 2011-09-02 |
| Genre | : Science |
| ISBN | : 0080475035 |
Download Cellular Electron Microscopy Book in PDF, ePub and Kindle
Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going
| Author | : Thijs Aartsma |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 538 |
| Release | : 2008-02-01 |
| Genre | : Science |
| ISBN | : 1402082509 |
Download Biophysical Techniques in Photosynthesis Book in PDF, ePub and Kindle
Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.
