Three Dimensional Electron Microscopy PDF Download
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| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 303 |
| Release | : 2019-07-18 |
| Genre | : Science |
| ISBN | : 0128170190 |
Download Three-Dimensional Electron Microscopy Book in PDF, ePub and Kindle
Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the Three-Dimensional Electron Microscopy technique
| Author | : Frank Joachim |
| Publisher | : Elsevier |
| Total Pages | : 361 |
| Release | : 1996-01-24 |
| Genre | : Science |
| ISBN | : 0080525814 |
Download Three-Dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have been instrumental in exploring the three-dimensional structure of* the nuclear pore complex* the calcium release channel;* the ribosome* chaperonins
| Author | : Joachim Frank |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 398 |
| Release | : 2013-04-17 |
| Genre | : Science |
| ISBN | : 1475721633 |
Download Electron Tomography Book in PDF, ePub and Kindle
This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.
| Author | : Joachim Frank |
| Publisher | : Oxford University Press |
| Total Pages | : 427 |
| Release | : 2006-02-02 |
| Genre | : Science |
| ISBN | : 0198034385 |
Download Three-Dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
| Author | : Joachim Frank |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 464 |
| Release | : 2008-03-05 |
| Genre | : Science |
| ISBN | : 0387690085 |
Download Electron Tomography Book in PDF, ePub and Kindle
This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series. Special emphasis is placed on the problems presented by limitations of data collection in the transmission electron microscope. The book, extensively revised and updated, takes the reader from biological specimen preparation to three-dimensional images of the cell and its components.
| Author | : Gabor T. Herman |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 275 |
| Release | : 2014-01-29 |
| Genre | : Mathematics |
| ISBN | : 1461495210 |
Download Computational Methods for Three-Dimensional Microscopy Reconstruction Book in PDF, ePub and Kindle
Approaches to the recovery of three-dimensional information on a biological object, which are often formulated or implemented initially in an intuitive way, are concisely described here based on physical models of the object and the image-formation process. Both three-dimensional electron microscopy and X-ray tomography can be captured in the same mathematical framework, leading to closely-related computational approaches, but the methodologies differ in detail and hence pose different challenges. The editors of this volume, Gabor T. Herman and Joachim Frank, are experts in the respective methodologies and present research at the forefront of biological imaging and structural biology. Computational Methods for Three-Dimensional Microscopy Reconstruction will serve as a useful resource for scholars interested in the development of computational methods for structural biology and cell biology, particularly in the area of 3D imaging and modeling.
| Author | : Joachim Frank |
| Publisher | : |
| Total Pages | : 342 |
| Release | : 1996-01-01 |
| Genre | : Science |
| ISBN | : 9780122650406 |
Download Three-dimensional Electron Microscopy of Macromolecular Assemblies Book in PDF, ePub and Kindle
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Key Features * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of * the nuclear pore complex * the calcium release channel; * the ribosome * chaperonins
| Author | : Henning Friis Poulsen |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 176 |
| Release | : 2004-08-31 |
| Genre | : Nature |
| ISBN | : 9783540223306 |
Download Three-Dimensional X-Ray Diffraction Microscopy Book in PDF, ePub and Kindle
Three-dimensional x-ray diffraction (3DXRD) microscopy is a novel experimental method for structural characterisation of polycrystalline materials. The position, morphology, phase, strain and crystallographic orientation of hundreds of grains or sub-grain embedded within mm-cm thick specimens can be determined simultaneously. Furthermore, the dynamics of the individual structural elements can be monitored during typical processes such as deformation or annealing. The book gives a comprehensive account of the methodology followed by a summary of selected applications. The method is presented from a mathematical/crystallographic point-of-view but with sufficient hands-on details to enable the reader to plan his or her own experiments. The scope of applications includes work in materials science and engineering, geophysics, geology, chemistry and pharmaceutical science.
| Author | : Florian Hauer |
| Publisher | : |
| Total Pages | : 119 |
| Release | : 2009 |
| Genre | : |
| ISBN | : |
Download Three-dimensional Electron Microscopy of Structurally Heterogeneous Biological Macromolecules Book in PDF, ePub and Kindle
| Author | : |
| Publisher | : |
| Total Pages | : |
| Release | : 2009 |
| Genre | : |
| ISBN | : |
Download Automated Three-dimensional Electron Microscopy Data Acquisition for High-throughput Applications of Frozen Hydrated Biological Samples Book in PDF, ePub and Kindle
Investigations of frozen-hydrated biological samples using the transmission electron microscope is a challenging task. In cell and structural biology large data sets of consistent quality are required. To record the necessary amount of micrographs in an efficient and timely manner, automated procedures are essential to control the electron microscope. The aim of this work was the development of a software package that enables the user to acquire micrographs of frozen-hydrated biological samples in an automated and unsupervised way for single-particle and tomography applications. The foundations of automation of an electron microscope and the necessary algorithms are presented, furthermore the application of the software is illustrated based on examples from single particle investigations and tomography.
