The Function Of The S2 Hinge Of Myosin Heavy Chain In Drosophila Melanogaster PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The Function Of The S2 Hinge Of Myosin Heavy Chain In Drosophila Melanogaster PDF full book. Access full book title The Function Of The S2 Hinge Of Myosin Heavy Chain In Drosophila Melanogaster.
Author | : Jennifer Anne Suggs |
Publisher | : |
Total Pages | : 118 |
Release | : 2003 |
Genre | : |
ISBN | : |
Download The Function of the S2 Hinge of Myosin Heavy Chain in Drosophila Melanogaster Book in PDF, ePub and Kindle
Author | : Becky Marlene Miller |
Publisher | : |
Total Pages | : 336 |
Release | : 2004 |
Genre | : Drosophila melanogaster |
ISBN | : |
Download Functional Analysis of Drosophila Melanogaster Muscle Myosin Heavy Chain Alternative Domains Book in PDF, ePub and Kindle
Drosophila melanogaster has a single myosin alkali light chain gene which encodes for two protein isoforms by developmentally regulated alternative splicing of the primary transcript. All six of the exons in the gene are present in the mRNA of larval muscles and the tubular and abdominal muscles of the adults. A novel mRNA species present exclusively in the adult and pupal Indirect Flight Muscle (IFM) lacks the fifth exon, thus encoding a MLC-ALK isoform with a variant carboxyl terminus. All introns of the transcript contain the established concensus splicing signals with the exception of intron 4. In this intron, a non-canonical polypurine stretch replaces the concensus polypyrimidine, rendering it a likely regulatory site. Because the transcripts are colinear with the gene throughout development the alternative splicing pattern in the IFM appears to be regulated at the level of splice site choice. The goal of this research is to identify the cis-regulatory sequences that control the choice between alternative larval and IFM-specific splicing pathways. I have developed a transient expression system for Drosophila Schneider 2 cultured cells utilizing the Drosophila metallothionein promoter to direct transcription of transfected MLC-ALK minigenes. This analysis demonstrated that the larval-specific splicing pathway represents the default splicing of the MLC-ALK transcripts. Analysis of mutant minigene transcripts revealed that splicing in the IFM-specific pathway is not the result of blockage or incapacitation of either splice acceptor or/and donor sequences flanking exon 5. The structures of the mutant mRNAs suggest that utilization of the IFM-specific pathway requires trans-acting factors which are absent in the cultured cells. Furthermore, analysis of mutant and hybrid minigene transcripts identified a unique cis-regulatory sequence proximal to the splice donor of intron 4, required for efficient utilization of the larval-specific splicing pathway. Mutations in intron 4 inhibit removal of the downstream intron 5 suggesting that an ordered pathway of intron removal is employed for larval-specific splicing. On the basis of these results a model of the mechanism of tissue and temporal regulation of alternative splicing of the MLC-ALK transcripts is presented.
Author | : Patrick Thomas O'Donnell |
Publisher | : |
Total Pages | : 278 |
Release | : 1988 |
Genre | : Drosophila melanogaster |
ISBN | : |
Download Genetic, Molecular and Ultrastructural Characterization of Myosin Heavy Chain Mutations in Drosophila Melanogaster Book in PDF, ePub and Kindle
Author | : Andrew Scott Ketchum |
Publisher | : |
Total Pages | : 498 |
Release | : 1991 |
Genre | : Drosophila melanogaster |
ISBN | : |
Download Molecular Analysis of the Drosophila Melanogaster Cytoplasmic Myosin Heavy Chain Book in PDF, ePub and Kindle
Author | : Kien Ai Trinh |
Publisher | : |
Total Pages | : 118 |
Release | : 1995 |
Genre | : Drosophila melanogaster |
ISBN | : |
Download Tissue-specific Regulatory Elements of the Myosin Heavy Chain Gene in Drosophila Melanogaster Book in PDF, ePub and Kindle
Author | : Venetia Lynne Collier |
Publisher | : |
Total Pages | : 190 |
Release | : 1988 |
Genre | : Drosophila melanogaster |
ISBN | : |
Download Molecular Analysis of Muscle Myosin Heavy Chain Gene Mutations in Drosophila Melanogaster Book in PDF, ePub and Kindle
Author | : Lynne M. Coluccio |
Publisher | : Springer Science & Business Media |
Total Pages | : 498 |
Release | : 2007-11-15 |
Genre | : Science |
ISBN | : 1402065191 |
Download Myosins Book in PDF, ePub and Kindle
This highly authoritative volume highlights the remarkable superfamily of molecular motors called myosins, which are involved in such diverse cellular functions as muscle contraction, intracellular transport, cell migration and cell division. In a timely compilation of chapters written by leading research groups that have made key discoveries in the field, the current understanding of the molecular mechanisms and biological functions of these intriguing proteins is explored.
Author | : Connie Jo Hansen |
Publisher | : |
Total Pages | : 192 |
Release | : 1986 |
Genre | : Genetic translation |
ISBN | : |
Download Analysis of Alternative Processing of Myosin Heavy Chain Transcripts in Drosophila Melanogaster Book in PDF, ePub and Kindle
Author | : Julien Ochala |
Publisher | : Frontiers Media SA |
Total Pages | : 163 |
Release | : 2017-05-22 |
Genre | : |
ISBN | : 2889451860 |
Download Myofilament Function in Health and Disease Book in PDF, ePub and Kindle
The present E-book, consisting of a compilation of original articles and reviews, presents how myofilaments are regulated in cardiac and skeletal muscles and trigger contraction. Additionally, this E-book gives insights into their dysregulation in a number of muscle disorders.
Author | : Michelle Mardahl-Dumesnil |
Publisher | : |
Total Pages | : 428 |
Release | : 1998 |
Genre | : Drosophila melanogaster |
ISBN | : |
Download The Roles of Myosin Heavy Chain, Enolase, and Paramyosin in Muscle Assembly and Function in Drosophila Melanogaster Book in PDF, ePub and Kindle
Manipulation of muscle genes to cause their under-, over- and mis-expression and subsequent assessment of resultant phenotypes offers a comprehensive approach to understand muscle assembly, development and function. These techniques are readily applied to the fruit fly, Drosophila melanogaster, because of the relative ease of mutant isolation and germ-line transformation. The consequences of altered muscle gene expression on muscle function and ultrastructure can be well characterized in this genetic system. This dissertation describes experiments to examine the roles of two thick filament proteins and a metabolic enzyme on Drosophila muscle structure and function. In the first chapter, I have determined the genetic lesion for the Mhc2 mutant and performed detailed ultrastructural analysis of the indirect flight muscle (IFM) of mutant and transgenic lines. This investigation reveals the negative effects of over-expression and under-expression of the Mhc gene on muscle function and structure. In Chapter Two, I characterize an enhancer detection line that exhibits strong IFM specific reporter gene activity. The P element of the enhancer detection line lies downstream of the enolase gene. Two interesting complementation groups result when the P element is used to mutagenize this locus. One complementation group is the first identification of a Drosophila enolase mutant, and the other is an unknown mutation that affects flight ability presumably by disrupting mitochondrial function in the IFM. In Chapter Three, I identify both standard (PM) and mini-paramyosin (mPM) mutants. Although thick filaments are present in embryonic body-wall muscle that is lacking PM, the sarcomere is unordered, indicating that PM is needed for its normal structure and function. Low levels of mPM significantly impair flight ability and viability. In addition, more thick filaments incorporate into IFM myofibrils of the mPM mutant than those of wild-type. Over-expression of either PM or mPM affects IFM structure and function. It also appears that equivalent stoichiometric levels of mPM and PM are important for correct sarcomeric structure in the IFM. From these studies, we determine that both PM and mPM confer specific structural qualities to the thick filament and myofibril morphology.