Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download The Development Of Fluorescence Probes For Cancer Cell Lines And Research On Catalytic Reactions PDF full book. Access full book title The Development Of Fluorescence Probes For Cancer Cell Lines And Research On Catalytic Reactions.
| Author | : Zijuan Zhang |
| Publisher | : |
| Total Pages | : 794 |
| Release | : 2012 |
| Genre | : Cancer cells |
| ISBN | : |
| Author | : Elli Kohen |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 442 |
| Release | : 2012-12-06 |
| Genre | : Medical |
| ISBN | : 146155845X |
Fluorescence is a very powerful tool for work at the frontier of cell biology, photobiology and bioinstrumentation. The stated aim of the workshop was to highlight the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particulary in terms of the analytical use of fluorescent probes in oncology. In the organization of the workshop a multidisciplinary approach was selected. The purpose of the Advanced Research Workshop (ARW) was to bring together researchers in the various disciplines of tissue optics, imaging, microspectrofluorometry and state of the art probes, in order to explore the full benefits that can be derived in biomedicine through the convergence of these approaches. When applied to in vivo and in situ studies, fluorescence and related optical methods enable us to explore within tissues, cells and organelles photon effects previously understood only in solution photochemistry. Processes which can be studied at the molecular level by photophysics, photochemistry and physical chemistry can be evaluated in living tissue by fluorescence spectroscopy and imaging at the intracellular level in terms of structure and function. Thus, fluorescence adds a new dimension to cell biology and physiology. This approach is now supported by a full and versatile, rapidly growing armamentarium of new selective probes for organelles, enzymes, cations, cytoskeleton and metabolic control.
| Author | : Joseph G Hirschberg |
| Publisher | : World Scientific |
| Total Pages | : 735 |
| Release | : 2002-05-20 |
| Genre | : Medical |
| ISBN | : 178326215X |
This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book./a
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| Total Pages | : |
| Release | : 2017 |
| Genre | : |
| ISBN | : |
Abstract : Carbohydrate transporters or GLUTs of the major facilitator superfamily (MFS) are responsible for transporting sugars into the cell and have been of research interest for decades. Disruptions, mutations, and over-activations of GLUTs have been linked to a number of major diseases including cancer, obesity, and diabetes. Differentiating between transporters is incredibly difficult due to highly conserved structures, and so specific targeting between transporters has proven a complex challenge. GLUTs are highly flexible in their conformations however exactly what will and will not pass through the transporter is ambiguous at best, and many attempt to target these transporters have failed. In an attempt to further understand GLUT5's transport capacity and specificity several probes were created by conjugating 1-amino-2,5-anhydro-D-mannitol with a number of fluorescent coumarins. These probes were then tested in cancer and normal breast cell lines to determine uptake mechanisms and transport specificity. To determine transport specificity probes were tested in the presence of competitive and non-competitive inhibitors. Probe analysis was carried out by evaluating the gained fluorescence of treated cells in a microplate setting and through confocal microscopy. Confocal imaging and Z-stack was utilized to understand the ability of the probe to pass into the cytosol or to remain in the cellular membrane. As a result, probes reflecting uptake capacity vs. membrane expression of the transporter were developed. The cumulative analysis of structure-uptake relationship for the developed probes gives insight into the capability of GLUT5 cargo transport and as well as a method for imaging GLUT5 in the cellular membrane.
| Author | : Ke Huang |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2022 |
| Genre | : Enzymatic analysis |
| ISBN | : |
Cyclic ADR-Ribose Synthases (cADPRSs) are one of the NAD-consuming enzymes. It hydrolyzes and cyclizes NAD+ into ADPR and cADPR, which are both Ca2+ channel regulators. Since the activities of cADPRSs involves in multiple biological process, monitoring the activities of these enzymes can provide useful information for studying pathology of the diseases that are closely associated with these enzymes. In this thesis, we have designed and synthesized a series of small molecule probes which can undergo base exchange reaction with the nicotinamide group of NAD+ in the presence of CD38 or activated SARM1. A large red shift of emission wavelength occurred after the base exchange reaction, which provides a powerful tool for detecting activities of this class of enzymes. In the first project, these probes were applied to the detection of the activities of SARM1. Among the 23 probes prepared, PC6 and PC11 showed excellent sensitivity and selectivity in vitro. They are cell-permeant, yet the resulting exchange products are im-permeant, allowing imaging of activated SARM1 in live cells. PC6 has provided the first evidence that SARM1 activation precedes axon degeneration by several hours in live DRG neurons. Moreover, it was also applied in the library screening for SARM1 inhibitor. Dehydronitrosonisodipine (dHNN) was found to has the inhibition ability to SARM1 activation, which is also the first compound ever reported that can inhibit SARM1 activation. PC11 has better fluorescent properties than PC6. With larger absorption and emission wavelength, PC11 provided the first approach for imaging SARM1 activation in vivo. In the second project, we focused on the study of the catalytic mechanism of CD38. Based on the preliminary results of the theoretical studies, we proposed that CD38 catalyzed cyclization and hydrolysis of NAD+ may involve an epoxide intermediate. In this mechanistic study, we have employed our newly developed probe PC6, CD38 mutant and different model compounds. The results of this study strongly supported the hypothesis of an epoxide intermediate in CD38-catalyzed reactions.
| Author | : Miroslav Dramićanin |
| Publisher | : Woodhead Publishing |
| Total Pages | : 304 |
| Release | : 2018-04-21 |
| Genre | : Technology & Engineering |
| ISBN | : 0081020309 |
Luminescence Thermometry: Methods, Materials, and Applications presents the state-of-the art applications of luminescence thermometry, giving a detailed explanation of luminescence spectroscopic schemes for the read-out of temperature, while also describing the diverse materials that are capable of sensing temperature via luminescence. Chapters cover the fundamentals of temperature, traditional thermometers and their figures of merit, a concise description of optical thermometry methods, luminescence and instrumentation, and an explanation of the ways in which increases in temperature quench luminescence. Additional sections focus on materials utilized for luminescence thermometry and the broad range of applications for luminescence thermometry, including temperature measurement at the nanoscale and the application of multifunctional luminescent materials. Provides an overview of luminescence thermometry applications, including high-temperature, biomedical, nanoscale and multifunctional Delves into luminescence thermometry by materials group, including Rare-earth and transition Metal Ion Doped, Semiconductors, Quantum Dots and Organic materials Gives a concise introduction of the latest methods of temperature measurement, including luminescence spectroscopic schemes and methods of analysis
| Author | : Zhenning Sun |
| Publisher | : |
| Total Pages | : |
| Release | : 2017-01-27 |
| Genre | : |
| ISBN | : 9781361427873 |
This dissertation, "Studies on Fluorescent Probes for the Specific Detection of Reactive Oxygen Species and Reactive Nitrogen Species in Living Cells" by Zhenning, Sun, 孫振宁, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled STUDIES ON FLUORESCENT PROBES FOR THE SPECIFIC DETECTION OF REACTIVE OXYGEN SPECIES AND REACTIVE NITROGEN SPECIES IN LIVING CELLS Submitted by SUN Zhen-Ning for the Degree of Doctor of Philosophy at The University of Hong Kong in September 2006 This project mainly focuses on the development of fluorescent probes for two biologically important oxidants - peroxynitrite and hypochlorite. On the basis of the similar chemical behavior between peroxynitrite - - (ONOO ) and peroxymonosulfate (HOOSO ), a reaction specific to peroxynitrite has been discovered (Scheme 1). On the basis of this specific reaction, several BODIPY-type fluorescent probes have been designed and synthesized according to the photoinduced electron transfer (PET) mechanism (Scheme 2). Among those probes, SZN-2 was found to be highly sensitive in specific detection of peroxynitrite 1 -- without interference from other competing oxidants, such as H O, O, NO, O, 2 2 2 2 and alkylperoxyl radical (ROO). Upon treatment with 7 equiv of peroxynitrite, probe SZN-2 showed up to 69-fold increase in fluorescence intensity. A linear correlation between emission intensity and concentration of peroxynitrite was observed. Moreover, the formation of peroxynitrite in living cells (J744.1 macrophages) can be visualized selectively by using probe SZN-2. This novel fluorescent probe should significantly facilitate studies of the biological roles of peroxynitrite in oxidative stress and many peroxynitrite-associated human diseases, such as rheumatoid arthritis, heart disease, atherosclerosis, and stroke. A fluorescent probe, SZN-3, has also been developed for the detection of hypochlorite on the basis of a specific reaction between methoxyphenol and hypochlorite (Scheme 3). This probe is highly sensitive and specific to hypochlorite. Upon treatment with 5 equiv of hypochlorite, probe SZN-3 showed up to 754-fold increase in fluorescence intensity. The reactivities of SZN-3 toward different reactive oxygen species (ROS) and reactive nitrogen species (RNS), including H O, 2 2 1 -- - - - - O, NO, O, OH, OCl, ONOO and alkylperoxyl radical (ROO ) were compared. 2 2 The results showed that fluorescence increased only upon reaction with OCl, demonstrating that probe SZN-3 has much higher selectivity towards hypochlorite than other ROS and RNS in an abiotic system. Moreover, using probe SZN-3, the formation of OCl has been successfully detected in an enzymatic system - (Myeloperoxidase/H O /Cl system) and in macrophages upon stimulation. This 2 2 new probe has provided an efficient tool for the evaluation of the roles of OCl in biological systems. Scheme 1 ONOO O OH CF CF RO 2.2 R = Me 2.1a R = H 3.23 Oxidant CF 3 No Reaction H O, OH, NO 2 2 RO 1 . O O 2, 2 R = Me 2.1a R = H 3.23 Scheme 2 O O CF CF 3 3 RO MeO NN NN B B Et NOC CONEt Et NOC CONEt F F F F 2 2 2 2 3.8 R = Me 2.14 (SZN-1) R = MOM 3.22 3.29 (SZN-2) R = H OH CF CF HO ONOO NN NN Et NOC CONEt Et NOC CONEt 2 2 F F 2 2 F F 3.29 (SZN-2) 2.15 non-fluorescent fluorescent Scheme 3 O O OMe OCl O O OH 4.1 4.2 OMe Oxidant No Reaction H O, OH, NO 2 2 1 . O O 2, 2 OH OMe
| Author | : Raphael Marcel Franzini |
| Publisher | : |
| Total Pages | : |
| Release | : 2010 |
| Genre | : |
| ISBN | : |
Templated fluorescence activation is an elegant nucleic acid detection technique, which relies on a target-activated chemical reaction between two oligonucleotide probes, eliciting a fluorescence readout. This method reports on genetic markers in solution phase and in cells. The research described in this thesis was aimed to expand the reaction scope of templated fluorescence activation and to develop probes, which overcome shortcomings of previous designs. Examples of DNA template-mediated fluorogenic reactions developed during this project include an organomercury-induced cyclization of Rhodamine thiosemicarbazide and the deprotection of a 7-azidomethoxycoumarin profluorophore by a Staudinger reaction. The most promising probe design (Q-STAR probes) is based on the reductive cleavage of an [alpha]-azidoether quencher release linker conjugated to a fluorophored-labeled DNA. A triphenylphosphine DNA-probe rapidly activates Q-STAR probes in the presence of the matched DNA target strand, reporting its presence by a strong fluorescence turn-on signal. Q-STAR probes are inert to aqueous conditions and cellular components, properties that were suboptimal for previous probe designs. Q-STAR probes report the target with single nucleotide specificity and enable an amplified detection signal by harnessing the target as a catalyst for the templated reaction. Q-STAR probes efficiently detect the presence of rRNAs in bacteria and mammalian cells. The probes are responsive to single nucleotide differences, which allows discriminating bacteria species by genetic variations using fluorescence microscopy or flow cytometry. Similarly, rRNAs in mammalian cells generate a strong fluorescence turn-on signal for Q-STAR probes. Templated fluorescence activation schemes bear considerable promise for applications in clinical diagnostics and molecular biology. The probe designs described in this thesis, in particular Q-STAR probes, constitute a major advancement in the field and will help achieve these goals.
| Author | : Benjamin F. Cravatt |
| Publisher | : Springer |
| Total Pages | : 417 |
| Release | : 2019-01-25 |
| Genre | : Medical |
| ISBN | : 3030111431 |
This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.
| Author | : Alexander Birbrair |
| Publisher | : Springer Nature |
| Total Pages | : 213 |
| Release | : 2020-06-25 |
| Genre | : Medical |
| ISBN | : 3030445186 |
Revealing essential roles of the tumor microenvironment in cancer progression, this book provides a comprehensive overview of the latest research in the field. A variety of topics are covered, including metabolism in the tumor microenvironment, stellate cells and endothelial progenitors in the tumor microenvironment, as well as the effects of HIV, viral hepatitis, and inflammation in the tumor microenvironment, and more. Taken alongside its companion volumes, Tumor Microenvironment: State of the Science updates us on what we know about various aspects of the tumor microenvironment, as well as future directions. This book is essential reading for advanced cell biology and cancer biology students as well as researchers seeking an update on research in the tumor microenvironment.
