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The Alfalfa Genome

The Alfalfa Genome
Author: Long-Xi Yu
Publisher: Springer Nature
Total Pages: 296
Release: 2021-07-17
Genre: Science
ISBN: 3030744663

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This book is the first comprehensive compilation of deliberations on whole genome sequencing of the diploid and tetraploid alfalfa genomes including sequence assembly, gene annotation, and comparative genomics with the model legume genome, functional genomics, and genomics of important agronomic characters. Other chapters describe the genetic diversity and germplasm collections of alfalfa, as well as development of genetic markers and genome-wide association and genomic selection for economical important traits, genome editing, genomics, and breeding targets to address current and future needs. Altogether, the book contains about 300 pages over 16 chapters authored by globally reputed experts on the relevant field in this crop. This book is useful to the students, teachers, and scientists in the academia and relevant private companies interested in genetics, breeding, pathology, physiology, molecular genetics and breeding, biotechnology, and structural and functional genomics. The work is also useful to seed and forage industries.


Genomic Investigations of Autumn Dormancy in Alfalfa (Medicago Sativa)

Genomic Investigations of Autumn Dormancy in Alfalfa (Medicago Sativa)
Author: Gitanshu Munjal
Publisher:
Total Pages:
Release: 2017
Genre:
ISBN: 9780355764505

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The primary goal of the research presented in the following chapters was to assess the genomic basis of the adaptive trait of autumn dormancy in alfalfa using high resolution genetic mapping approaches in diverse germplasm. Previous work in the field has developed protocols to generate vast amounts of sequence based marker data for this species using GBS. However, opportunity remains to extract more information from such data than had been previously possible. In chapter 1, we review some concepts relevant to the rest of this work including basic biology and improvement of alfalfa, the trait of autumn dormancy, and opportunities for molecular markers in alfalfa research. In chapter 2, we employ a recent SNP calling methodology and develop a framework for estimating allele frequencies from pooled sequencing. We use that framework to screen the non-dormant cultivar CUF 101 and populations developed by three cycles of selection for taller and shorter plants in autumn. We validated the robustness of our GBS-derived, population-specific allele frequency estimates using an analytic approach. In chapter 3, we analyze pre- and post-selection populations from an additional five backgrounds together with CUF 101 to seek evidence for loci under selection in germplasm expressing a range of dormancy levels. In chapter 4, we build on recent scientific reports to propose a method for conducting genome-wide association scans from low-coverage autotetraploid sequencing data that is able to account for uncertainty regarding genotypes. We apply this method to a panel of semi-dormant individuals from a commercial breeding program. Using simulations, we show this method to be more powerful than an existing method.


Genetic Analysis of Complex Traits in Alfalfa (Medicago Sativa L.)

Genetic Analysis of Complex Traits in Alfalfa (Medicago Sativa L.)
Author: Joseph Gary Robins
Publisher:
Total Pages: 260
Release: 2004
Genre:
ISBN:

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The genetic structure of complex agronomic traits in alfalfa (Medicago sativa) is not well understood. By crossing the subspecies M. sativa subsp. falcata and M. sativa subsp. sativa, a fullsib F1 population was created from which a genetic linkage map of each parental genome was developed using RFLP and SSR markers. These maps include simplex, duplex, and simplex-simplex alleles along with a number of alleles exhibiting segregation distortion. The inclusion of these more complicated segregation ratios resulted in greater saturation of the genome, a better convergence to eight consensus linkage groups, and a more realistic view of regions of the genome that may not behave normally due to segregation distortion than would have been possible by only using simplex alleles as has been done previously. The population was clonally propagated and grown at three field locations with phenotypic data collected over three years for various agronomic traits, including biomass production, forage height, and forage regrowth. Combining the marker data with the phenotypic data, markers were identified from each parental genome that were associated with these traits, suggesting that both major germplasm sources of cultivated alfalfa contain alleles that may contribute to improved alfalfa cultivars. These results provide a much better understanding of the genomic regions underlying these traits and are an important start in efforts aimed at the use of marker-assisted selection for the improvement of alfalfa cultivars.


Histone H3 Genes in Alfalfa

Histone H3 Genes in Alfalfa
Author: Anthony J. Robertson
Publisher:
Total Pages: 422
Release: 1994
Genre: Alfalfa
ISBN:

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Gene Flow in Alfalfa

Gene Flow in Alfalfa
Author: Allen Emile Van Deynze
Publisher:
Total Pages: 40
Release: 2008
Genre: Alfalfa
ISBN:

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"Although there have been instances in which low-level presence of regulated materials has resulted in market disruptions (e.g., Starlink corn, LL601 and LL604 rice), there is no evidence of significant market disruption associated with the commercialization of deregulated biotech traits in the United States. Concurrent with increased U.S. farmer adoption of biotech traits in corn, soybean, and cotton there have been increases in U.S. grain/fiber export (USDA-FAS 2007) and organic production (USDA-NASS 2007a, b, c). Although only 3 to 5% of the U.S. alfalfa hay production is sold to GE-sensitive markets (Putnam 2006), production for these markets has significant economic importance in specific regions of the United States. Approximately 33% of U.S. alfalfa seed production is exported, primarily to GE-sensitive markets. A thorough understanding of gene flow in alfalfa is critical to establishing stewardship programs that enable coexistence between alfalfa growers producing GE alfalfa hay or seed and growers producing these products for GE-sensitive markets. Understanding the relative importance of gene flow between and within feral plants, hay, and seed production fields helps to identify key biological, agricultural, and environmental barriers to gene flow and to formulate logical mitigation strategies for managing the AP of GE traits in non-GE alfalfa seed and hay. Synchrony in flowering, presence of pollinators, isolation distance, and relative abundance of pollen between pollen source and pollen recipient plants are typical biological barriers, most of which are amenable to management in hay and/or seed production systems. In general, it seems that NAFA Best Management Practices in hay and certified alfalfa seed production, coupled with the pollinator-specific isolation guidelines outlined in the NAFA Best Management Practices document, are adequate for managing AP to tolerance levels appropriate for most markets. These types of management practices are employed successfully by producers of certified seed in most crops, including alfalfa, to ensure genetic purity of seed stocks. Increased isolation distances in seed production--including production in non-GE seed production zones--use of border areas, crop rotation, use of certified seed, careful selection of the introduced pollinator, and routine elimination of neighboring feral alfalfa plants are tools that can be applied to decrease further the risk of gene flow in the production of seed for GE-sensitive markets."--Summary.