Test No 455 Draft Performance Based Test Guideline For Stably Transfected Transactivation In Vitro Assays To Detect Estrogen Receptor Agonists And Antagonists PDF Download

Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Test No 455 Draft Performance Based Test Guideline For Stably Transfected Transactivation In Vitro Assays To Detect Estrogen Receptor Agonists And Antagonists PDF full book. Access full book title Test No 455 Draft Performance Based Test Guideline For Stably Transfected Transactivation In Vitro Assays To Detect Estrogen Receptor Agonists And Antagonists.

Test No. 455: Draft Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists

Test No. 455: Draft Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists
Author:
Publisher:
Total Pages: 54
Release: 2015
Genre:
ISBN:

Download Test No. 455: Draft Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle

This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays). It comprises mechanistically and functionally similar test methods for the identification of estrogen receptor agonists and antagonists and should facilitate the development of new similar or modified test methods. The two reference test methods that provide the basis for this PBTG are: the Stably Transfected TA (STTA) assay using the (h) ERÎł-HeLa-9903 cell line, derived from a human cervical tumor, and the BG1Luc ER TA assay using the BG1Luc-4E2 cell line, derived from a human ovarian adenocarcinoma. The cell lines used in these assays express ER and have been stably transfected with an ER responsive luciferase reporter gene. The assays are used to identify chemicals that activate (id est act as agonists) and also suppress (id est act as antagonists) ER- dependent transcription. ER are activated following ligand binding, after which the receptor-ligand complex binds to specific DNA response elements and transactivates the reporter gene, resulting in increased cellular expression of a marker enzyme (e.g. luciferase in luciferase based systems). The enzyme then transforms the substrate to a bioluminescent product that can be quantitatively measured with a luminometer. These test methods are being proposed for screening and prioritisation purposes, but also provide mechanistic information that can be used in a weight of evidence approach.


Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists

Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists
Author: OECD
Publisher: OECD Publishing
Total Pages: 53
Release: 2015-07-28
Genre:
ISBN: 9264243046

Download Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle

This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays).


Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists

Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists
Author: OECD
Publisher: OECD Publishing
Total Pages: 39
Release: 2012-10-02
Genre:
ISBN: 9264185380

Download Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists Book in PDF, ePub and Kindle

This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provides the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists (ER TAs). It comprises mechanistically and functionally similar test ...


OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists
Author: OECD
Publisher: OECD Publishing
Total Pages: 74
Release: 2021-06-17
Genre:
ISBN: 9264265295

Download OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists Book in PDF, ePub and Kindle

This Performance-Based Test Guideline (PBTG) describes in vitro assays, which provide the methodology of Stably Transfected Transactivation to detect Estrogen Receptor Agonists and Antagonists (ER TA assays).


Alternative Toxicological Methods

Alternative Toxicological Methods
Author: Harry Salem
Publisher: CRC Press
Total Pages: 616
Release: 2003-03-26
Genre: Medical
ISBN: 0203008790

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Bringing together the recent and relevant contributions of over 125 scientists from industry, government, and academia in North America and Western Europe, Alternative Toxicological Methods explores the development and validation of replacement, reduction, and refinement alternatives (the 3Rs) to animal testing. Internationally recognized scientist


OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 442E: In Vitro Skin Sensitisation In Vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome Pathway for Skin Sensitisation

OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 442E: In Vitro Skin Sensitisation In Vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome Pathway for Skin Sensitisation
Author: OECD
Publisher: OECD Publishing
Total Pages: 91
Release: 2024-06-25
Genre:
ISBN: 9264264353

Download OECD Guidelines for the Testing of Chemicals, Section 4 Test No. 442E: In Vitro Skin Sensitisation In Vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome Pathway for Skin Sensitisation Book in PDF, ePub and Kindle

The present Key Event based Test Guideline (TG) addresses the human health hazard endpoint skin sensitisation, following exposure to a test chemical. Skin sensitisation refers to an allergic response following skin contact with the tested chemical, as defined by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). This TG is proposed to address the activation of dendritic cells, which is one Key Event on the Adverse Outcome Pathway (AOP) for Skin Sensitisation. It provides four in vitro test methods addressing the same Key Event on the AOP: (i) the human cell Line Activation Test or h-CLAT method, (ii) the U937 Cell Line Activation Test or U-SENS, (iii) the Interleukin-8 Reporter Gene Assay or IL-8 Luc assay and (iv) the Genomic Allergen Rapid Detection for assessment of skin sensitisers (GARDTMskin). All of them are used for supporting the discrimination between skin sensitisers and non-sensitisers in accordance with the UN GHS. The test methods described in this TG either quantify the change in the expression of cell surface marker(s) CD54 and CD86, the cytokine IL-8, or a series of genes (genomic biomarker signature) that are associated with the process of activation of monocytes and DC following exposure to sensitisers.


Hayes' Principles and Methods of Toxicology, Sixth Edition

Hayes' Principles and Methods of Toxicology, Sixth Edition
Author: A. Wallace Hayes
Publisher: CRC Press
Total Pages: 2186
Release: 2014-10-10
Genre: Medical
ISBN: 1842145363

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Hayes’ Principles and Methods of Toxicology has long been established as a reliable reference to the concepts, methodologies, and assessments integral to toxicology. The new sixth edition has been revised and updated while maintaining the same high standards that have made this volume a benchmark resource in the field. With new authors and new chapters that address the advances and developments since the fifth edition, the book presents everything toxicologists and students need to know to understand hazards and mechanisms of toxicity, enabling them to better assess risk. The book begins with the four basic principles of toxicology—dose matters, people differ, everything transforms, and timing is crucial. The contributors discuss various agents of toxicity, including foodborne, solvents, crop protection chemicals, radiation, and plant and animal toxins. They examine various methods for defining and measuring toxicity in a host of areas, including genetics, carcinogenicity, toxicity in major body systems, and the environment. This new edition contains an expanded glossary reflecting significant changes in the field. New topics in this edition include: The importance of dose–response Systems toxicology Food safety The humane use and care of animals Neurotoxicology The comprehensive coverage and clear writing style make this volume an invaluable text for students and a one-stop reference for professionals.


Adverse Effects of Engineered Nanomaterials

Adverse Effects of Engineered Nanomaterials
Author: Bengt Fadeel
Publisher: Academic Press
Total Pages: 488
Release: 2017-02-14
Genre: Medical
ISBN: 0128094907

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Adverse Effects of Engineered Nanomaterials: Exposure, Toxicology, and Impact on Human Health, Second Edition, provides a systematic evaluation of representative engineered nanomaterials (ENM) of high volume production and their high economic importance. Each class of nanomaterials discussed includes information on what scientists, industry, regulatory agencies, and the general public need to know about nanosafety. Written by leading international experts in nanotoxicology and nanomedicine, this book gives a comprehensive view of the health impact of ENM, focusing on their potential adverse effects in exposed workers, consumers, and patients. All chapters have been updated with new sections on the endocrine system and other organ systems. In addition, other newly added sections include introductory chapters on the physio-chemical characterization of nanomaterials and interactions between nanomaterials and biological systems, as well as a new chapter that explores risk assessment and management of nanomaterials. This book fills an important need in terms of bridging the gap between experimental findings and human exposure to ENM, also detailing the clinical and pathological consequences of such exposure in the human population. Uses a schematic, non-exhaustive approach to summarizes the most important research data in this field Discusses the health implications of experimental data in nanotoxicology Presents a completely revised edition that focuses on the human health impacts of engineered nanomaterials, including many organ-specific chapters


Bioanalytical Tools in Water Quality Assessment

Bioanalytical Tools in Water Quality Assessment
Author: Beate Escher
Publisher: IWA Publishing
Total Pages: 273
Release: 2011-12-15
Genre: Science
ISBN: 1843393689

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Part of Water Quality Set - Buy all four books and save over 30% on buying separately! Bioanalytical Tools in Water Quality Assessment reviews the application of bioanalytical tools to the assessment of water quality including surveillance monitoring. The types of water included range from wastewater to drinking water, including recycled water, as well as treatment processes and advanced water treatment. Bioanalytical Tools in Water Quality Assessment not only demonstrates applications but also fills in the background knowledge in toxicology/ecotoxicology needed to appreciate these applications. Each chapter summarises fundamental material in a targeted way so that information can be applied to better understand the use of bioanalytical tools in water quality assessment. Bioanalytical tools in Water Quality Assessment can be used by lecturers teaching academic and professional courses and also by risk assessors, regulators, experts, consultants, researchers and managers working in the water sector. It can also be a reference manual for environmental engineers, analytical chemists, and toxicologists. Authors: Beate Escher, National Research Centre for Environmental Toxicology (EnTox), The University of Queensland, Australia, Frederic Leusch, Smart Water Research Facility (G51), Griffith University Gold Coast Campus, Australia. With contributions by Heather Chapman and Anita Poulsen