Techniques In Mammalian Cell Culture PDF Download

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General Techniques of Cell Culture

General Techniques of Cell Culture
Author: Maureen A. Harrison
Publisher: Cambridge University Press
Total Pages: 178
Release: 1997-10-13
Genre: Science
ISBN: 9780521574969

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Concise introduction to a major technique of cell biology laboratories for those new to the field.


Animal Cell Culture Methods

Animal Cell Culture Methods
Author:
Publisher: Academic Press
Total Pages: 389
Release: 1998-07-03
Genre: Science
ISBN: 0080859550

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This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features * Reviews basic principles of cell culture * Gives options and techniques on how to look at cells


Cell Culture Techniques

Cell Culture Techniques
Author: Michael Aschner
Publisher: Humana
Total Pages: 302
Release: 2020-05-10
Genre: Medical
ISBN: 9781493992300

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This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.


Advanced Mammalian Cell Culture Techniques

Advanced Mammalian Cell Culture Techniques
Author: Km Ramkumar
Publisher: CRC Press
Total Pages: 0
Release: 2023-10
Genre:
ISBN: 9781032494524

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This up-to-date book compiles the basic and advanced laboratory techniques of mammalian cell culture. It provides elaborate stepwise protocols, key points, and troubleshooting tips.


Animal Cell Culture

Animal Cell Culture
Author: John M. Davis
Publisher: John Wiley & Sons
Total Pages: 389
Release: 2011-03-16
Genre: Science
ISBN: 0470975636

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This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.


Advanced Mammalian Cell Culture Techniques

Advanced Mammalian Cell Culture Techniques
Author: K.M. Ramkumar
Publisher: CRC Press
Total Pages: 129
Release: 2023-10-25
Genre: Science
ISBN: 100096938X

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This up-to-date book compiles both basic and advanced laboratory techniques of mammalian cell culture. It is divided into four major sections encompassing the basics of cell culture, nucleic acid and protein isolation, cell-staining techniques, and cell transfection and single-cell analysis. The topics include aseptic handling, media preparation, and passaging of cells. The book also outlines downstream assays such as nucleic acid and protein isolation from in-vitro cell cultures. Key Features: Covers cellular staining using fluorescent dyes, genetic manipulation of cells via transfection, and an introduction to single-cell analyses Discusses basics in cell culture and downstream applications including gene and protein expression analysis Includes the principles underlying each of the techniques and provides a detailed methodology to practice Explores the whole range of techniques – from basic to downstream applications and advanced methods The book is essential for students and researchers in the field of life sciences, biotechnology, genetics, and molecular biology.


Basic Cell Culture Protocols

Basic Cell Culture Protocols
Author: Jeffrey W. Pollard
Publisher: Springer Science & Business Media
Total Pages: 477
Release: 1997
Genre: Animals
ISBN: 0896034410

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Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.


Culture of Animal Cells

Culture of Animal Cells
Author: R. Ian Freshney
Publisher: Wiley-Liss
Total Pages: 510
Release: 1993-12-29
Genre: Science
ISBN: 9780471589662

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This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports


Molecular Biology of The Cell

Molecular Biology of The Cell
Author: Bruce Alberts
Publisher:
Total Pages: 0
Release: 2002
Genre: Cytology
ISBN: 9780815332183

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