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Genome Engineering via CRISPR-Cas9 System

Genome Engineering via CRISPR-Cas9 System
Author: Vijai Singh
Publisher: Academic Press
Total Pages: 0
Release: 2020-02-21
Genre: Science
ISBN: 9780128181409

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Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.


Targeted Genome Engineering via CRISPR/Cas9 in Plants

Targeted Genome Engineering via CRISPR/Cas9 in Plants
Author: Alvina Gul
Publisher: Elsevier
Total Pages: 466
Release: 2024-07-26
Genre: Science
ISBN: 0443266158

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Targeted Genome Engineering via CRISPR/Cas9 in Plants provides in-depth insights into the use of the emerging “CRISPR/Cas9” technology for precise genome editing. This technology has revolutionized plant science research particularly for crop improvement owing to its simplicity and efficiency. The book provides a wide range of CRISPR/Cas9 gene editing techniques for a variety of plants. Chapters include the latest applications of CRISPR/Cas9 system in connection with abiotic stresses, biotic stresses, biofortification, yield improvement, disease modelling and prognosis and molecular diagnosis. Targeted Genome Engineering via CRISPR/Cas9 in Plants also evaluates various regulatory and ethical aspects that must be considered when implementing the CRISPR/Cas9 approach. This book is a valuable resource for professionals and researchers, as it provides effective CRISPR/Cas9-based strategies for sustainable agriculture and treatment of various diseases. Explains basic mechanism and implementation of CRISPR/Cas9 technology in a wide range of plants. Provides practical guidance on the applications of CRISPR/Cas9 in different scientific disciplines of plant science. Discusses the risks and challenges of genome engineering.


Plant Genome Editing with CRISPR Systems

Plant Genome Editing with CRISPR Systems
Author: Yiping Qi
Publisher: Humana
Total Pages: 0
Release: 2019-01-05
Genre: Science
ISBN: 9781493989904

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This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.


Genome Engineering via CRISPR-Cas9 System

Genome Engineering via CRISPR-Cas9 System
Author: Vijai Singh
Publisher: Academic Press
Total Pages: 386
Release: 2020-02-18
Genre: Science
ISBN: 0128181419

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Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more


Gene Editing in Plants

Gene Editing in Plants
Author: Ashwani Kumar
Publisher: Springer Nature
Total Pages: 871
Release:
Genre:
ISBN: 9819985293

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Basics of CRISPR/Cas Mediated Plant Genome Editing

Basics of CRISPR/Cas Mediated Plant Genome Editing
Author: Anshu Alok
Publisher: CRC Press
Total Pages: 329
Release: 2024-08-23
Genre: Science
ISBN: 1040112137

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CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture free editing which makes it easier for crop improvement. With such technologies, application of genome editing has opened new paths for crop improvements and sustainable agriculture. The current book will be useful for beginners to understand these tools and how to use them in a better and more efficient way.


CRISPR-Cas Systems

CRISPR-Cas Systems
Author: Rodolphe Barrangou
Publisher: Springer Science & Business Media
Total Pages: 300
Release: 2012-12-13
Genre: Science
ISBN: 364234657X

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CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.


The Use of CRISPR/Cas9, ZFNs, and TALENs in Generating Site-specific Genome Alterations

The Use of CRISPR/Cas9, ZFNs, and TALENs in Generating Site-specific Genome Alterations
Author: Jennifer A. Doudna
Publisher: Academic Press
Total Pages: 549
Release: 2014
Genre: Medical
ISBN: 9780128011850

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease


Genome Editing in Plants

Genome Editing in Plants
Author: Om Prakash Gupta
Publisher: CRC Press
Total Pages: 382
Release: 2021-07-25
Genre: Science
ISBN: 1000410196

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Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops


Targeted Genome Editing in Crops

Targeted Genome Editing in Crops
Author: Huanbin Zhou
Publisher: Frontiers Media SA
Total Pages: 137
Release: 2021-10-25
Genre: Science
ISBN: 288971554X

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