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Studying the Plant-microbe Interface of Populus Using Constructed Microbial Communities

Studying the Plant-microbe Interface of Populus Using Constructed Microbial Communities
Author: Aditya Barde
Publisher:
Total Pages: 0
Release: 2019
Genre:
ISBN:

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The association of plant and microbes at the root-soil interface exemplifies a complex, multi-organism system that is shaped by the participating organisms and environmental forces. The plant microbe interface is a dynamic boundary across which a plant detects, interacts with, and may alter its associated biotic environment in order to maintain or improve its performance. Poor understanding of the mechanics of the plant-microbe interface represents a critical knowledge gap. Our goal was to investigate key areas of this gap: (a) microbial community assembly dynamics on Populus host root systems, (b) potential host specificity of two Populus species, and (c) the effect of environmental factors in structuring the root microbiome of Populus. This study used constructed communities in which specific microbes are combined with an axenic host in a controlled fashion. The process used two communities of 10 bacterial strains isolated from two poplar species; the 10 bacterial strains represented abundant members both functionally and phylogenetically from Populus natural microbiomes. The two communities were inoculated onto two Populus host species, and microbial community structure and abundance was assayed by qPCR and/or 16S rRNA amplicon sequencing. A time course study revealed that Pantoea dominates the community at all sampled time points and Paraburkholderia emerges as a dominant member as time progresses. In addition, species of Populus were dominated by Paraburkholderia and Pantoea or Rahnella strains regardless of original host species isolated from. Community members colonized in similar abundances compared to colonization by individual members of the communities. The shade treatment had no effect on the structure of the bacterial community, although stem length and root area of the plant increased significantly with the 10-member community. This study demonstrates the feasibility and analysis of model communities to study microbiome function in plant systems.


Plant Microbe Interface

Plant Microbe Interface
Author: Ajit Varma
Publisher: Springer
Total Pages: 415
Release: 2019-08-02
Genre: Science
ISBN: 3030198316

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This book shares the latest insights into the genetic basis of molecular communication between plants and their microbial consortia. Further, the book highlights the capabilities of the rhizosphere and endosphere, which help manage ecosystem responses to climate change, nutrient cycling and sequestration of carbon; and discusses their application to the development and management of renewable energy sources. In their natural environments, plants are surrounded by a tremendous number of microorganisms. Some microbes directly interact with plants in a mutually beneficial fashion, while others colonize plants solely for their own advantage. In addition, microbes can indirectly affect plants by drastically altering their environments. Understanding the complex nature of the plant-microbe interface (PMI) can pave the way for novel strategies to improve plant productivity in an eco-friendly manner. The PMI approach focuses on understanding the physical, molecular, and chemical interactions between organisms in order to determine their functional roles in biological, physical, chemical and environmental systems. Although several metabolites from plants and microbes have now been fully characterized, their roles in chemical interactions between these associates remain poorly understood, and require further investigation.


The plant microbiome and its importance for plant and human health

The plant microbiome and its importance for plant and human health
Author: Martin Grube
Publisher: Frontiers E-books
Total Pages: 190
Release: 2015-01-22
Genre: Botany
ISBN: 2889193780

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The study of plant-microbe associations by new techniques has significantly improved our understanding of the structure and specificity of the plant microbiome. Yet, microbiome function and the importance of the plant’s microbiome in the context of human and plant health are largely unexplored. Comparable with our human microbiome, millions of microbes inhabit plants, forming complex ecological communities that influence plant growth and health through its collective metabolic activities and host interactions. Viewing the microbiota from an ecological perspective can provide insight into how to promote plant health and stress tolerance of their hosts or how to adapt to a changing climate by targeting this microbial community. Moreover, the plant microbiome has a substantial impact on human health by influencing our gut microbiome by eating raw plants such as lettuce and herbs but also by influencing the microbiome of our environment through airflow. This research topic comprising reviews, original and opinion articles highlights the current knowledge regarding plant microbiomes, their specificity, diversity and function as well as all aspects studying the management of plant microbiomes to enhance plant growth, health quality and stress tolerance.


Advances in Biology and Ecology of Nitrogen Fixation

Advances in Biology and Ecology of Nitrogen Fixation
Author: Takuji Ohyama
Publisher: BoD – Books on Demand
Total Pages: 286
Release: 2014-01-29
Genre: Science
ISBN: 9535112163

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Biological nitrogen fixation has essential role in N cycle in global ecosystem. Several types of nitrogen fixing bacteria are recognized: the free-living bacteria in soil or water; symbiotic bacteria making root nodules in legumes or non-legumes; associative nitrogen fixing bacteria that resides outside the plant roots and provides fixed nitrogen to the plants; endophytic nitrogen fixing bacteria living in the roots, stems and leaves of plants. In this book there are 11 chapters related to biological nitrogen fixation, regulation of legume-rhizobium symbiosis, and agriculture and ecology of biological nitrogen fixation, including new models for autoregulation of nodulation in legumes, endophytic nitrogen fixation in sugarcane or forest trees, etc. Hopefully, this book will contribute to biological, ecological, and agricultural sciences.


Beneficial Plant-Bacterial Interactions

Beneficial Plant-Bacterial Interactions
Author: Bernard R. Glick
Publisher: Springer Nature
Total Pages: 390
Release: 2020-06-08
Genre: Science
ISBN: 303044368X

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This book provides a straightforward and easy-to-understand overview of beneficial plant-bacterial interactions. It features a wealth of unique illustrations to clarify the text, and each chapter includes study questions that highlight the important points, as well as references to key experiments. Since the publication of the first edition of Beneficial Plant-Bacterial Interactions, in 2015, there has been an abundance of new discoveries in this area, and in recent years, scientists around the globe have begun to develop a relatively detailed understanding of many of the mechanisms used by bacteria that facilitate plant growth and development. This knowledge is gradually becoming an integral component of modern agricultural practice, with more and more plant growth-promoting bacterial strains being commercialized and used successfully in countries throughout the world. In addition, as the world’s population continues to grow, the pressure for increased food production will intensify, while at the same time, environmental concerns, mean that environmentally friendly methods of food production will need to replace many traditional agricultural practices such as the use of potentially dangerous chemicals. The book, intended for students, explores the fundamentals of this new paradigm in agriculture, horticulture, and environmental cleanup.


Rhizosphere Engineering

Rhizosphere Engineering
Author: Ramesh Chandra Dubey
Publisher: Academic Press
Total Pages: 566
Release: 2022-02-15
Genre: Technology & Engineering
ISBN: 0323885950

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Rhizosphere Engineering is a guide to applying environmentally sound agronomic practices to improve crop yield while also protecting soil resources. Focusing on the potential and positive impacts of appropriate practices, the book includes the use of beneficial microbes, nanotechnology and metagenomics. Developing and applying techniques that not only enhance yield, but also restore the quality of soil and water using beneficial microbes such as Bacillus, Pseudomonas, vesicular-arbuscular mycorrhiza (VAM) fungi and others are covered, along with new information on utilizing nanotechnology, quorum sensing and other technologies to further advance the science. Designed to fill the gap between research and application, this book is written for advanced students, researchers and those seeking real-world insights for improving agricultural production. Explores the potential benefits of optimized rhizosphere Includes metagenomics and their emerging importance Presents insights into the use of biosurfactants


Plant Microbiomes for Sustainable Agriculture

Plant Microbiomes for Sustainable Agriculture
Author: Ajar Nath Yadav
Publisher: Springer Nature
Total Pages: 496
Release: 2020-03-06
Genre: Technology & Engineering
ISBN: 3030384535

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This book encompasses the current knowledge of plant microbiomes and their potential biotechnological application for plant growth, crop yield and soil health for sustainable agriculture. The plant microbiomes (rhizospheric, endophytic and epiphytic) play an important role in plant growth, development, and soil health. Plant and rhizospheric soil are a valuable natural resource harbouring hotspots of microbes, and it plays critical roles in the maintenance of global nutrient balance and ecosystem function. The diverse group of microbes is key components of soil–plant systems, where they are engaged in an intense network of interactions in the rhizosphere/endophytic/phyllospheric. The rhizospheric microbial diversity present in rhizospheric zones has a sufficient amount of nutrients release by plant root systems in form of root exudates for growth, development and activities of microbes. The endophytic microbes are referred to those microorganisms, which colonize in the interior of the plant parts, viz root, stem or seeds without causing any harmful effect on host plant. Endophytic microbes enter in host plants mainly through wounds, naturally occurring as a result of plant growth, or through root hairs and at epidermal conjunctions. Endophytes may be transmitted either vertically (directly from parent to offspring) or horizontally (among individuals). The phyllosphere is a common niche for synergism between microbes and plant. The leaf surface has been termed as phyllosphere and zone of leaves inhabited by microorganisms as phyllosphere. The plant part, especially leaves, is exposed to dust and air currents resulting in the establishments of typical flora on their surface aided by the cuticles, waxes and appendages, which help in the anchorage of microorganisms. The phyllospheric microbes may survive or proliferate on leaves depending on extent of influences of material in leaf diffuseness or exudates. The leaf diffuseness contains the principal nutrients factors (amino acids, glucose, fructose and sucrose), and such specialized habitats may provide niche for nitrogen fixation and secretions of substances capable of promoting the growth of plants. The microbes associated with plant as rhizospheric, endophytic and epiphytic with plant growth promoting (PGP) attributes have emerged as an important and promising tool for sustainable agriculture. PGP microbes promote plant growth directly or indirectly, either by releasing plant growth regulators; solubilization of phosphorus, potassium and zinc; biological nitrogen fixation or by producing siderophore, ammonia, HCN and other secondary metabolites which are antagonistic against pathogenic microbes. The PGP microbes belong to different phylum of archaea (Euryarchaeota); bacteria (Acidobacteria, Actinobacteria, Bacteroidetes, Deinococcus-Thermus, Firmicutes and Proteobacteria) and fungi (Ascomycota and Basidiomycota), which include different genera namely Achromobacter, Arthrobacter, Aspergillus, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Burkholderia, Enterobacter, Erwinia, Flavobacterium, Gluconoacetobacter, Haloarcula, Herbaspirillum, Methylobacterium, Paenibacillus, Pantoea, Penicillium, Piriformospora, Planomonospora, Pseudomonas, Rhizobium, Serratia and Streptomyces. These PGP microbes could be used as biofertilizers/bioinoculants at place of chemical fertilizers for sustainable agriculture. The aim of “Plant Microbiomes for Sustainable Agriculture” is to provide the current developments in the understanding of microbial diversity associated with plant systems in the form of rhizospheric, endophytic and epiphytic. The book is useful to scientist, research and students related to microbiology, biotechnology, agriculture, molecular biology, environmental biology and related subjects.


How Plants Communicate with their Biotic Environment

How Plants Communicate with their Biotic Environment
Author: Guillaume Becard
Publisher: Academic Press
Total Pages: 406
Release: 2017-03-17
Genre: Science
ISBN: 0128016205

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How Plants Communicate with Their Biotic Environment addresses how plants perceive the presence of organisms (other plants, microbes, insects and nematodes) living in their proximity, how they manage to be attractive when these organisms are friendly, and how they defend themselves from foes. Specific chapters delve into ecology and defense mechanisms, allelopathy and the role of allelochemicals in plant defense, plant signaling, and plant communication with microbes and animals, including herbivores. In addition, the book presents discussions on communication and its role in plant pollination. This comprehensive resource presents tactics that can be taken from the lab, to the bench, to the forest. Gathers, under a common general outline, a comprehensive knowledge issued from distinct scientific communities Combines three life science disciplines, including ecology, evolutionary biology, and molecular biology Addresses a topical subject as the natural biological processes described represent basic knowledge that help develop low input sustainable agriculture Written by renowned scientists in their field