Structural Studies On Membrane Active Peptides In Lipid Bilayers By Solid State 19f Nmr PDF Download
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Author | : Sergiy E. Afonin |
Publisher | : |
Total Pages | : 198 |
Release | : 2004 |
Genre | : |
ISBN | : |
Download Structural Studies on Membrane Active Peptides in Lipid Bilayers by Solid State 19F-NMR Book in PDF, ePub and Kindle
Author | : Miguel A. R. B. Castanho |
Publisher | : Internat'l University Line |
Total Pages | : 675 |
Release | : 2010 |
Genre | : Medical |
ISBN | : 0972077456 |
Download Membrane-active Peptides Book in PDF, ePub and Kindle
Author | : Myungwoon Lee |
Publisher | : |
Total Pages | : 241 |
Release | : 2018 |
Genre | : |
ISBN | : |
Download Structure and Dynamics of Membrane Proteins from Solid-state NMR Book in PDF, ePub and Kindle
Solid-state nuclear magnetic resonance (SSNMR) spectroscopy is an essential tool to elucidate the structure, dynamics, and function of biomolecules. This thesis mainly focuses on the structure determination of the hydrophobic domains for fusion proteins which are involved in membrane fusion between the cell membrane and viral envelope. Although extensive structural studies have been conducted on the soluble ectodomain by crystallography, the structural topologies of the hydrophobic TMD of the fusion proteins have been poorly understood. Here, we introduced SSNMR to investigate the secondary structure and oligomeric states of the TMD of two fusion proteins, PIV5 F and HIV gp41. For the PIV5 TMD, the membrane dependent secondary structure was determined by measuring the chemical shifts: predominant a-helical conformation in the POPC/cholesterol membrane shifts to the [Beta]-strand in the POPE membrane. Using 19F spin diffusion experiments on the fluorinated TMD, we have determined that the TMD forms a trimeric helical bundle. For the HIV gp4l MPER-TMD, we found the presence of a turn between the MPER helix and the TMD helix by measuring intramolecular distances and probing the lipid-peptide and water-peptide interactions. Intermolecular 19F- 19F distances of the fluorinated peptides indicate that the MPER-TMD is a trimeric. In addition to membrane fusion proteins, we have studied the oligomeric structure and the zinc-bound coordination geometry of a de novo designed amyloid fibril that catalyzes ester hydrolysis. By measuring the intermolecular contacts, we determined that peptides form parallel-in- register P-sheets and further assemble into stacked bilayers in an antiparallel orientation. The zinc binding sites were confirmed by the chemical shifts perturbation of histidines with zinc and the specific zinc-bound geometry was identified by measuring intra-residue distances of histidines. We also investigated the effects of cryoprotectants on the spectral resolution of lipid membranes and membrane peptides at low temperature. 13C and 1H MAS spectra of various cryoprotected membranes showed that DMSO provides the best resolution enhancement with the best ice formation retardation at low temperature and DLPE lipid exhibits the excellent resolution.
Author | : Ming Tang |
Publisher | : |
Total Pages | : |
Release | : 2008 |
Genre | : |
ISBN | : |
Download Solid-state NMR Studies of the Interactions Between Cationic Membrane Peptides and Lipid Bilayers Book in PDF, ePub and Kindle
Solid-state NMR studies of the interactions between cationic membrane peptides and lipid bilayers.
Author | : Katherine Anne Henzler Wildman |
Publisher | : |
Total Pages | : 660 |
Release | : 2003 |
Genre | : |
ISBN | : |
Download The Mechanism of Lipid Bilayer Disruption by the Human Antimicrobial Peptide, LL-37 Book in PDF, ePub and Kindle
Author | : Sidney A. Simon |
Publisher | : Academic Press |
Total Pages | : 606 |
Release | : 2002-11-13 |
Genre | : Science |
ISBN | : 0080925855 |
Download Peptide-Lipid Interactions Book in PDF, ePub and Kindle
This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
Author | : Jerry C. C. Chan |
Publisher | : Springer |
Total Pages | : 328 |
Release | : 2011-10-12 |
Genre | : Science |
ISBN | : 3642248039 |
Download Solid State NMR Book in PDF, ePub and Kindle
Dipolar Recoupling, by Niels Chr. Nielsen, Lasse A. Strassø and Anders B. Nielsen.- Solid-State NMR Techniques for the Structural Determination of Amyloid Fibrils, by Jerry C. C. Chan.- Solid-State 19F-NMR of Peptides in Native Membranes, by Katja Koch, Sergii Afonin, Marco Ieronimo, Marina Berditsch and Anne S. Ulrich.- Probing Quadrupolar Nuclei by Solid-State NMR Spectroscopy: Recent Advances, by Christian Fernandez and Marek Pruski.- Solid State NMR of Porous Materials Zeolites and Related Materials, by Hubert Koller and Mark Weiß.- Solid-State NMR of Inorganic Semiconductors, by James P. Yesinowski.-
Author | : Bercem Dutagaci |
Publisher | : |
Total Pages | : 472 |
Release | : 2013 |
Genre | : |
ISBN | : |
Download Biophysical Studies of Lipid Membranes by Solid State NMR and Molecular Dynamics Simulations Book in PDF, ePub and Kindle
Author | : Thomas Peters |
Publisher | : Springer |
Total Pages | : 244 |
Release | : 2009-11-04 |
Genre | : Science |
ISBN | : 3540490809 |
Download Bioactive Conformation II Book in PDF, ePub and Kindle
This series presents critical reviews of the present position and future trends in modern chemical research. It contains short and concise reports on chemistry, each written by the world renowned experts. The volume is still valid and useful after five or ten years. More information, as well as the electronic version of the whole content, is available at: springerlink.com.
Author | : The Nuclear Magnetic Resonance Society of Japan |
Publisher | : Springer |
Total Pages | : 634 |
Release | : 2017-11-23 |
Genre | : Science |
ISBN | : 9811059667 |
Download Experimental Approaches of NMR Spectroscopy Book in PDF, ePub and Kindle
This book describes the advanced developments in methodology and applications of NMR spectroscopy to life science and materials science. Experts who are leaders in the development of new methods and applications of life and material sciences have contributed an exciting range of topics that cover recent advances in structural determination of biological and material molecules, dynamic aspects of biological and material molecules, and development of novel NMR techniques, including resolution and sensitivity enhancement. First, this book particularly emphasizes the experimental details for new researchers to use NMR spectroscopy and pick up the potentials of NMR spectroscopy. Second, the book is designed for those who are involved in either developing the technique or expanding the NMR application fields by applying them to specific samples. Third, the Nuclear Magnetic Resonance Society of Japan has organized this book not only for NMR members of Japan but also for readers worldwide who are interested in using NMR spectroscopy extensively.