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Stimulated Raman Scattering Microscopy

Stimulated Raman Scattering Microscopy
Author: Ji-Xin Cheng
Publisher: Elsevier
Total Pages: 612
Release: 2021-12-04
Genre: Science
ISBN: 0323903371

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Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics


Stimulated Raman Scattering Microscopy

Stimulated Raman Scattering Microscopy
Author: Ji-Xin Cheng
Publisher: Elsevier
Total Pages: 610
Release: 2021-12-08
Genre: Science
ISBN: 0323851584

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Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics


Coherent Raman Scattering Microscopy

Coherent Raman Scattering Microscopy
Author: Ji-Xin Cheng
Publisher: CRC Press
Total Pages: 603
Release: 2016-04-19
Genre: Science
ISBN: 1439867666

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The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc


Advancements in Stimulated Raman Scattering Microscopy

Advancements in Stimulated Raman Scattering Microscopy
Author: Benjamin Figueroa (Jr.)
Publisher:
Total Pages: 135
Release: 2020
Genre:
ISBN:

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Stimulated Raman scattering (SRS) microscopy has transformed our ability to image chemical and biological systems because of its sub-micrometer resolution and molecular specificity. However, limitations in small spectral coverage, poor spectral resolution, and low sensitivity hamper the ability of SRS to investigate complex systems. Furthermore, the usage of SRS microscopy beyond biological systems is still very limited. Here I present my work which specifically addresses these issues. In chapters 2 and 3 I demonstrate the benefits of using SRS microscopy outside of its current biological niche, by investigating materials such as latent fingerprints and pharmaceutical tablets. In chapter 4 I address the limitations of poor spectral resolution and small spectral coverage by developing a parabolic fiber amplifier to provide a better SRS microscope. In chapter 5 I demonstrate an application of the developed fiber amplifier system for monitoring and quantifying temperature at the microscale level. Lastly, in chapter 6 I introduce the development of a new optical parametric oscillator (OPO) source to address the final limitation by increasing the sensitivity of SRS imaging, which ties into the future outlook on advancements needed in SRS microscopy.


Advances in Stimulated Raman Scattering Microscopy Via Deep Learning

Advances in Stimulated Raman Scattering Microscopy Via Deep Learning
Author: Bryce Adrian Manifold
Publisher:
Total Pages: 0
Release: 2022
Genre:
ISBN:

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Stimulated Raman scattering (SRS) microscopy is a powerful chemical imaging technique that acquires images based on the vibrational-spectral "fingerprints" of molecules within an imaged field of view often without the need for exogenous fluorophores or labels. SRS microscopy has found an established niche in biophotonics with many examples of translational clinical applications and demonstrations of imaging various biological systems on subcellular to tissue spatial orders. Concurrent to the development of SRS microscopy, computational advancements have seen a democratized adoption of deep learning platforms for a wide variety of computer vision tasks. In this work I document my contributions in integrating SRS microscopy and deep learning towards advancing the capability to study biological systems. Specifically, deep learning will be shown to address technical limitations of SRS microscopy such as imaging noise and ultimate imaging depth in tissue samples. Deep learning will also be shown to improve analysis of SRS images via the development of a novel convolutional neural network architecture designed to handle a variety of chemical imaging techniques and perform a variety of computer vision tasks. Finally, I will show how these advancements and novel architecture can be used to diagnose thyroid cancer in label-free human tissue samples and to classify and study T cells based on label-free images.


Stimulated Raman Scattering

Stimulated Raman Scattering
Author: N. G. Basov
Publisher: Springer
Total Pages: 156
Release: 1982
Genre: Science
ISBN:

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Label-free DNA Imaging in Vivo with Stimulated Raman Scattering Microscopy

Label-free DNA Imaging in Vivo with Stimulated Raman Scattering Microscopy
Author:
Publisher:
Total Pages: 6
Release: 2015
Genre:
ISBN:

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Label-free DNA imaging is highly desirable in biology and medicine to perform live imaging without affecting cell function and to obtain instant histological tissue examination during surgical procedures. Here we show a label-free DNA imaging method with stimulated Raman scattering (SRS) microscopy for visualization of the cell nuclei in live animals and intact fresh human tissues with subcellular resolution. Relying on the distinct Raman spectral features of the carbon-hydrogen bonds in DNA, the distribution of DNA is retrieved from the strong background of proteins and lipids by linear decomposition of SRS images at three optimally selected Raman shifts. Based on changes on DNA condensation in the nucleus, we were able to capture chromosome dynamics during cell division both in vitro and in vivo. We tracked mouse skin cell proliferation, induced by drug treatment, through in vivo counting of the mitotic rate. Moreover, we demonstrated a label-free histology method for human skin cancer diagnosis that provides comparable results to other conventional tissue staining methods such as H & E. In conclusion, our approach exhibits higher sensitivity than SRS imaging of DNA in the fingerprint spectral region. Compared with spontaneous Raman imaging of DNA, our approach is three orders of magnitude faster, allowing both chromatin dynamic studies and label-free optical histology in real time.


Investigation of Biological Fibers Using Spontaneous and Stimulated Raman Techniques

Investigation of Biological Fibers Using Spontaneous and Stimulated Raman Techniques
Author: Rebecca Lynn Younger
Publisher:
Total Pages: 123
Release: 2012
Genre:
ISBN: 9781267416476

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Spontaneous Raman scattering, Stimulated Raman Scattering (SRS) microscopy and Coherent Anti-Stokes Raman Scattering (CARS) microscopy were used to investigate: the orientation of specific vibrational modes in cellulose fibers, the presence of ordered water adsorbed into cellulose fibers and the distribution of deuterated sulfopropylacrylate in keratin fibers. Through these investigations it was found that the bond orientations of the methine and hydroxy stretching modes in cellulose fibers are oriented perpendicular and parallel to the long axis of the fiber, respectively. Through humidity-controlled Raman spectroscopy experiments with D2O, ordered deuterium hydroxide was detected in cellulose fibers and was found to align parallel to the long axis of the fiber. As the humidity was increased, the anisotropy of the OD-stretching band decreased as expected. Furthermore, it was found that as the humidity was increased the anisotropy of the CH- and OH-stretching regions did not change, supporting the findings of an ordered intercalation of water molecules into the fiber structure. Finally, hyperspectral SRS and Raman spectroscopy were applied to the detection of deuterated compounds in treated human hair samples provided to us by The Proctor and Gamble Company. From this investigation SRS was found to be able to detect the distribution of deuterated sulfopropylacrylate on high-level treated hairs, demonstrating the potential of SRS as a useful detection method for applications in the cosmetic industry.


Optical Imaging in Human Disease and Biological Research

Optical Imaging in Human Disease and Biological Research
Author: Xunbin Wei
Publisher: Springer Nature
Total Pages: 309
Release: 2021-05-29
Genre: Medical
ISBN: 9811576270

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The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.