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Singlet Oxygen Detection and Imaging

Singlet Oxygen Detection and Imaging
Author: Steffen Hackbarth
Publisher:
Total Pages: 90
Release: 2021-03-25
Genre:
ISBN: 9781681739519

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Singlet Oxygen, the lowest electronically excited state of molecular oxygen, is highly reactive and involved in many chemical and biological processes. It is one major mediator during photosensitization, which has been used by mankind since ancient times, even though the mechanisms behind it were understood only about half a century ago. The combination of high reactivity and very long natural lifetime allows for direct optical detection of singlet oxygen and its interactions using its characteristic phosphorescence at around 1270 nm. Since this emission is very weak, optical detection was technically very challenging for a long time. Therefore, even today, most laboratories only exploit the high reactivity to observe the interaction with sensor molecules, rather than singlet oxygen emission itself. However, in recent years highly sensitive optical detection was developed, the authors being major contributors. This book is dedicated to the detection of singlet oxygen, discussing possibilities, pitfalls and limits of the various methods with a special focus on time-resolved phosphorescence and the kinetics of singlet oxygen generation and decay including involved and related processes, discussing investigated systems with various complexity from solutions over in vitro to in vivo. The long-standing paradigm that singlet oxygen phosphorescence is a benchmark for detection systems rather than an option for process observation is still ubiquitous and this book hopes to contribute in overcoming this still prevailing bias.


Singlet Oxygen Detection and Imaging

Singlet Oxygen Detection and Imaging
Author: Steffen Hackbarth
Publisher: Springer Nature
Total Pages: 85
Release: 2022-05-31
Genre: Science
ISBN: 3031023919

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Singlet Oxygen, the lowest electronically excited state of molecular oxygen, is highly reactive and involved in many chemical and biological processes. It is one major mediator during photosensitization, which has been used by mankind since ancient times, even though the mechanisms behind it were understood only about half a century ago. The combination of high reactivity and very long natural lifetime allows for direct optical detection of singlet oxygen and its interactions using its characteristic phosphorescence at around 1270 nm. Since this emission is very weak, optical detection was technically very challenging for a long time. Therefore, even today, most laboratories only exploit the high reactivity to observe the interaction with sensor molecules, rather than singlet oxygen emission itself. However, in recent years highly sensitive optical detection was developed, the authors being major contributors. This book is dedicated to the detection of singlet oxygen, discussing possibilities, pitfalls and limits of the various methods with a special focus on time-resolved phosphorescence and the kinetics of singlet oxygen generation and decay including involved and related processes, discussing investigated systems with various complexity from solutions over in vitro to in vivo. The long-standing paradigm that singlet oxygen phosphorescence is a benchmark for detection systems rather than an option for process observation is still ubiquitous and this book hopes to contribute in overcoming this still prevailing bias.


Singlet Oxygen

Singlet Oxygen
Author: Santi Nonell
Publisher: Royal Society of Chemistry
Total Pages: 348
Release: 2016-01-27
Genre: Science
ISBN: 1782626972

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Meeting the desire for a comprehensive book that collects and curates the vast amount of knowledge gained in the field of singlet oxygen, this title covers the physical, chemical and biological properties of this reactive oxygen species and also its increasingly important applications across chemical, environmental and biomedical areas.The editors have a long and distinguished background in the field of singlet oxygen chemistry and biomedical applications, giving them a unique insight and ensuring the contributions attain the highest scientific level.The book provides an up to date reference resource for both the beginner and experienced researcher and crucially for those working across disciplines such as photochemistry, photobiology and photomedicine.


Singlet Oxygen

Singlet Oxygen
Author: Santi Nonell
Publisher: Royal Society of Chemistry
Total Pages: 488
Release: 2016-01-27
Genre: Science
ISBN: 1782620389

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Meeting the desire for a comprehensive book that collects and curates the vast amount of knowledge gained in the field of singlet oxygen, this title covers the physical, chemical and biological properties of this reactive oxygen species and also its increasingly important applications across chemical, environmental and biomedical areas.The editors have a long and distinguished background in the field of singlet oxygen chemistry and biomedical applications, giving them a unique insight and ensuring the contributions attain the highest scientific level.The book provides an up to date reference resource for both the beginner and experienced researcher and crucially for those working across disciplines such as photochemistry, photobiology and photomedicine.


Quenched-phosphorescence Detection of Molecular Oxygen

Quenched-phosphorescence Detection of Molecular Oxygen
Author: Dmitri B Papkovsky
Publisher: Royal Society of Chemistry
Total Pages: 384
Release: 2018-05-25
Genre: Science
ISBN: 1788014553

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Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.


Quenched-phosphorescence Detection of Molecular Oxygen

Quenched-phosphorescence Detection of Molecular Oxygen
Author: Ruslan I Dmitriev
Publisher: Royal Society of Chemistry
Total Pages: 384
Release: 2018-05-30
Genre: Science
ISBN: 1788011759

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Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.


Photophysical Detection of Singlet Oxygen

Photophysical Detection of Singlet Oxygen
Author: ARNAB MAITY
Publisher:
Total Pages: 0
Release: 2019
Genre: Science
ISBN:

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The chemical reactivity of singlet oxygen (1O2) (SO) derives from its electronically excited state. Being a unique reactive oxygen species SO takes part in many important atmospheric, biological physical, chemical, and therapeutic process and attracted current research interest. To understand the mechanistic pathways in various process the detection and quantification of SO is very important. The direct method of detection is very challenging due to its highly reactive nature. Only direct method of determination of phosphorescence of SO at 1270¬†nm has been utilised but that also puts some limitation due to very low luminescence quantum yield. Indirect method using UV,ÄìVis spectrophotometric, fluorescent and chemiluminescent probes has been extensively studied for this purpose. Elucidation of various mechanistic processes improvised the use of sophisticated spectroscopic detection probe for SO have been discussed in a simple and lucid manner in this article through citation of literature examples. Four major spectroscopic methods i.e. spectrophotometry, fluorescence, emission and chemiluminescence are elaborately discussed with special emphasis to chemical probes having high selectivity and sensitivity for SO.


Handbook of Photosynthesis

Handbook of Photosynthesis
Author: Mohammad Pessarakli
Publisher: CRC Press
Total Pages: 2015
Release: 2018-09-03
Genre: Science
ISBN: 1315360195

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Since the publication of the previous editions of the Handbook of Photosynthesis, many new ideas on photosynthesis have emerged in the past decade that have drawn the attention of experts and researchers on the subject as well as interest from individuals in other disciplines. Updated to include 37 original chapters and making extensive revisions to the chapters that have been retained, 90% of the material in this edition is entirely new. With contributions from over 100 authors from around the globe, this book covers the most recent important research findings. It details all photosynthetic factors and processes under normal and stressful conditions, explores the relationship between photosynthesis and other plant physiological processes, and relates photosynthesis to plant production and crop yields. The third edition also presents an extensive new section on the molecular aspects of photosynthesis, focusing on photosystems, photosynthetic enzymes, and genes. New chapters on photosynthesis in lower and monocellular plants as well as in higher plants are included in this section. The book also addresses growing concerns about excessive levels and high accumulation rates of carbon dioxide due to industrialization. It considers plant species with the most efficient photosynthetic pathways that can help improve the balance of oxygen and carbon dioxide in the atmosphere. Completely overhauled from its bestselling predecessors, the Handbook of Photosynthesis, Third Edition provides a nearly entirely new source on the subject that is both comprehensive and timely. It continues to fill the need for an authoritative and exhaustive resource by assembling a global team of experts to provide thorough coverage of the subject while focusing on finding solutions to relevant contemporary issues related to the field.


Measuring Oxidants and Oxidative Stress in Biological Systems

Measuring Oxidants and Oxidative Stress in Biological Systems
Author: Lawrence J. Berliner
Publisher: Springer Nature
Total Pages: 237
Release: 2020-08-08
Genre: Science
ISBN: 303047318X

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This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.