Single Cell Protein Analysis PDF Download
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Author | : Anup K. Singh |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2015-11-07 |
Genre | : Science |
ISBN | : 9781493929863 |
Download Single Cell Protein Analysis Book in PDF, ePub and Kindle
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.
Author | : Aik T. Ooi |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2022-11-26 |
Genre | : Science |
ISBN | : 9781071617731 |
Download Single-Cell Protein Analysis Book in PDF, ePub and Kindle
This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.
Author | : Israel Goldberg |
Publisher | : Springer Science & Business Media |
Total Pages | : 197 |
Release | : 2013-03-13 |
Genre | : Science |
ISBN | : 3642465404 |
Download Single Cell Protein Book in PDF, ePub and Kindle
In early 1973, I returned to Israel from a post-doctoral fellowship at Harvard University, and was accepted as a lecturer in the Department of Applied Microbiology at the Hebrew University of Jerusalem. Shortly after my return, Professor Richard Mateles, who at that time was head of the Department, suggested that I purchase a good and comprehensive book on single cell protein (SCP) in order to expand my general knowledge in the subject I had started then to work on; that was microbial utilization of one-carbon (C ) compounds. l Naturally, I took his advice (after all, he was the Boss) and bought the book, which was the only general book published on this subject at that time, and was based on papers presented at the First International Conference on Single Cell Protein, held at the Massachussetts Institute of Technology (M.I.T.), on October 1967 (Mateles and Tannenbaum, editors) [1]. Through this book I became acquainted with the world's hunger problem that existed in the past, and ways in which it was to be solved by SCP products prepared from CO , fossil-based raw 2 materials, and from wastes.
Author | : Pratima Bajpai |
Publisher | : Springer |
Total Pages | : 85 |
Release | : 2017-07-28 |
Genre | : Technology & Engineering |
ISBN | : 9811058733 |
Download Single Cell Protein Production from Lignocellulosic Biomass Book in PDF, ePub and Kindle
This book focuses on bioconversion of lignocellulosic residues into single-cell protein, which offers an alternative to conventional proteins (such as soybean meal, egg protein or meat protein in animal feed) that is not affected by the climate. It provides an overview of the general uses of lignocellulosic residues and their bioconversion into single-cell protein using microorganisms, as well as the recovery of the valuable by-products. It also explores the benefits and potential drawbacks of single-cell protein, with an emphasis on the economic advantages of such processes. Given its multidisciplinary scope, the book represents a valuable resource for academics and industry practitioners interested in the production of single-cell protein from lignocellulosic residues.
Author | : Renjie Liao |
Publisher | : |
Total Pages | : 125 |
Release | : 2019 |
Genre | : Fluorescence in situ hybridization |
ISBN | : |
Download Highly Multiplexed Single Cell in Situ Protein Analysis with Cleavable Fluorescent Probes Book in PDF, ePub and Kindle
Measurements of different molecular species from single cells have the potential to reveal cell-to-cell variations, which are precluded by population-based measurements. An increasing percentage of researches have been focused on proteins, for its central roles in biological processes. Immunofluorescence (IF) has been a well-established protein analysis platform. To gain comprehensive insights into cell biology and diagnostic pathology, a crucial direction would be to increase the multiplexity of current single cell protein analysis technologies. An azide-based chemical cleavable linker has been introduced to design and synthesis novel fluorescent probes. These probes allow cyclic immunofluorescence staining which leads to the feasibility of highly multiplexed single cell in situ protein profiling. These highly multiplexed imaging-based platforms have the potential to quantify more than 100 protein targets in cultured cells and more than 50 protein targets in single cells in tissues. This approach has been successfully applied in formalin-fixed paraffin-embedded (FFPE) brain tissues. Multiplexed protein expression level results reveal neuronal heterogeneity in the human hippocampus.
Author | : Gemma Milman |
Publisher | : |
Total Pages | : |
Release | : 2015 |
Genre | : |
ISBN | : |
Download Towards Single Cell Protein Analysis of Cardiac Progenitor Cells Book in PDF, ePub and Kindle
Author | : Bruce Alberts |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cytology |
ISBN | : 9780815332183 |
Download Molecular Biology of The Cell Book in PDF, ePub and Kindle
Author | : Tao Huang |
Publisher | : Humana Press |
Total Pages | : 417 |
Release | : 2018-03-14 |
Genre | : Science |
ISBN | : 9781493977161 |
Download Computational Systems Biology Book in PDF, ePub and Kindle
This volume introduces the reader to the latest experimental and bioinformatics methods for DNA sequencing, RNA sequencing, cell-free tumour DNA sequencing, single cell sequencing, single-cell proteomics and metabolomics. Chapters detail advanced analysis methods, such as Genome-Wide Association Studies (GWAS), machine learning, reconstruction and analysis of gene regulatory networks and differential coexpression network analysis, and gave a practical guide for how to choose and use the right algorithm or software to handle specific high throughput data or multi-omics data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Systems Biology: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author | : |
Publisher | : |
Total Pages | : |
Release | : 2010 |
Genre | : |
ISBN | : |
Download Protein Analysis at the Single Cell Level by Nonlinear Laser Wave-Mixing Spectroscopy for High Throughput Capillary Electrophoresis Applications Book in PDF, ePub and Kindle
SADRI, BEHROKH BAGHERIFAR. Protein Analysis at the Single Cell Level by Nonlinear Laser Wave-Mixing Spectroscopy for High Throughput Capillary Electrophoresis Applications. (Under the direction of William G. Tong and Morteza G. Khaledi) Nonlinear degenerate four-wave mixing is presented as an ultrasensitive optical absorption-based method for detection and measurement of biological samples. Wave-mixing imaging detection technique can localize and quantify biomolecules in single cells and tissue sections with excellent spatial distribution of light absorbed by a target sample. Cellular components can be label-free or labeled with a chromophore or a fluorophore and imaged by wave mixing using a CCD camera. In a 2-D forward-scattering wave-mixing geometry, two overlapping laser beams form interference gratings and transfer their energy to an absorbing medium, creating thermal gratings followed by changes in the refractive index. The probe beam diffracts off these laser- induced gratings to produce the signal beam, which is detected by a CCD camera or a photodiode. A single bio cell can be placed in a glass slide and as the laser beams probe the labeled cellular component, the CCD camera captures wave-mixing signals corresponding to the absorbing cellular components. This nonlinear imaging technique can be used for both live and fixed cells in real time to obtain information on sequential changes in the number, morphology and distribution of cellular components in a single cell. Nonlinear laser wave-mixing spectroscopy coupled with capillary electrophoresis provides a novel ultrasensitive method for single-cell protein analysis. This method is used to detect proteins separated within a single cell. Nonlinear wave mixing has many advantages including quadratic dependency on analyte concentration, high spatial resolution and small sample requirements. Furthermore, wave mixing offers excellent detection sensitivity levels even when using very short optical path lengths, an.
Author | : Fan-Gang Tseng |
Publisher | : MDPI |
Total Pages | : 237 |
Release | : 2018-10-01 |
Genre | : Electronic book |
ISBN | : 3038421936 |
Download Single Cell Analysis in Biotechnology and Systems Biology Book in PDF, ePub and Kindle
This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS