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Signal Transduction Immunohistochemistry

Signal Transduction Immunohistochemistry
Author: Alexander E. Kalyuzhny
Publisher: Springer Nature
Total Pages: 333
Release: 2022-12-13
Genre: Science
ISBN: 1071628119

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This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.


Signal Transduction in Cancer

Signal Transduction in Cancer
Author: David A. Frank
Publisher: Springer Science & Business Media
Total Pages: 358
Release: 2002-12-31
Genre: Medical
ISBN: 1402073402

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One of the most exciting areas of cancer research now is the development of agents which can target signal transduction pathways that are activated inappropriately in malignant cells. The understanding of the molecular abnormalities which distinguish malignant cells from their normal counterparts has grown tremendously. This volume summarizes the current research on the role that signal transduction pathways play in the pathogenesis of cancer and how this knowledge may be used to develop the next generation of more effective and less toxic anticancer agents. Series Editor comments: "The biologic behavior of both normal and cancer cells is determined by critical signal transduction pathways. This text provides a comprehensive review of the field. Leading investigators discuss key molecules that may prove to be important diagnostic and/or therapeutic targets."


Protein Kinase-mediated Decisions Between Life and Death

Protein Kinase-mediated Decisions Between Life and Death
Author: Ayse Basak Engin
Publisher: Springer Nature
Total Pages: 415
Release: 2021-02-04
Genre: Medical
ISBN: 3030498441

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Protein phosphorylation via protein kinases is an inevitable process that alters physiological and pathological functions of the cells. Thus, protein kinases play key roles in the regulation of cell life or death decisions. Protein kinases are frequently a driving factor in a variety of human diseases including aging and cellular senescence, immune system and endothelial dysfunctions, cancers, insulin resistance, cholestasis and neurodegenerative diseases, as well as bacterial resistance in persistent infections. Recent developments in quantitative proteomics provide important opinions on kinase inhibitor selectivity and their modes of action in the biological context. Protein Kinase-mediated Decisions Between Life and Death aims to have the reader catch insights about up-to-date opinions on “Protein Kinases” related pathways that threaten human health and life. As “Protein Kinases” are related to many health problems, clinicians, basic science researchers and students need this information. Chapter “Signal Transduction in Immune Cells and Protein Kinases” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.


Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas

Handbook of Immunohistochemistry and in situ Hybridization of Human Carcinomas
Author: M. A. Hayat
Publisher: Elsevier
Total Pages: 261
Release: 2005-09-21
Genre: Science
ISBN: 0080456251

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Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer. This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today. * The only book available that translates molecular genetics into cancer diagnosis * The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations * Methods discussed were either developed or refined by expert contributors in their own laboratories


Immunohistochemistry

Immunohistochemistry
Author: Alexander E. Kalyuzhny
Publisher: Springer
Total Pages: 91
Release: 2016-03-24
Genre: Science
ISBN: 3319308939

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Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.


Cellular Signal Transduction in Toxicology and Pharmacology

Cellular Signal Transduction in Toxicology and Pharmacology
Author: Jonathan W. Boyd
Publisher: John Wiley & Sons
Total Pages: 336
Release: 2019-04-16
Genre: Science
ISBN: 1119060265

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Covering a key topic due to growing research into the role of signaling mechanisms in toxicology, this book focuses on practical approaches for informatics, big data, and complex data sets. Combines fundamentals / basics with experimental applications that can help those involved in preclinical drug studies and translational research Includes detailed presentations of study methodology and data collection, analysis, and interpretation Discusses tools like experimental design, sample handling, analytical measurement techniques


Neurobiology of Chemical Communication

Neurobiology of Chemical Communication
Author: Carla Mucignat-Caretta
Publisher: CRC Press
Total Pages: 614
Release: 2014-02-14
Genre: Medical
ISBN: 1466553413

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Intraspecific communication involves the activation of chemoreceptors and subsequent activation of different central areas that coordinate the responses of the entire organism—ranging from behavioral modification to modulation of hormones release. Animals emit intraspecific chemical signals, often referred to as pheromones, to advertise their presence to members of the same species and to regulate interactions aimed at establishing and regulating social and reproductive bonds. In the last two decades, scientists have developed a greater understanding of the neural processing of these chemical signals. Neurobiology of Chemical Communication explores the role of the chemical senses in mediating intraspecific communication. Providing an up-to-date outline of the most recent advances in the field, it presents data from laboratory and wild species, ranging from invertebrates to vertebrates, from insects to humans. The book examines the structure, anatomy, electrophysiology, and molecular biology of pheromones. It discusses how chemical signals work on different mammalian and non-mammalian species and includes chapters on insects, Drosophila, honey bees, amphibians, mice, tigers, and cattle. It also explores the controversial topic of human pheromones. An essential reference for students and researchers in the field of pheromones, this is also an ideal resource for those working on behavioral phenotyping of animal models and persons interested in the biology/ecology of wild and domestic species.


Retinoblastoma: New Insights for the Healthcare Professional: 2012 Edition

Retinoblastoma: New Insights for the Healthcare Professional: 2012 Edition
Author:
Publisher: ScholarlyEditions
Total Pages: 57
Release: 2012-12-10
Genre: Medical
ISBN: 1464974675

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Retinoblastoma: New Insights for the Healthcare Professional / 2012 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Retinoblastoma in a concise format. The editors have built Retinoblastoma: New Insights for the Healthcare Professional / 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Retinoblastoma in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Retinoblastoma: New Insights for the Healthcare Professional / 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.


Issues in Life Sciences—Molecular Biology: 2012 Edition

Issues in Life Sciences—Molecular Biology: 2012 Edition
Author:
Publisher: ScholarlyEditions
Total Pages: 927
Release: 2013-01-10
Genre: Science
ISBN: 1481645366

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Issues in Life Sciences—Molecular Biology / 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Molecular Biology. The editors have built Issues in Life Sciences—Molecular Biology: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Molecular Biology in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Life Sciences—Molecular Biology: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.


Proteomics in Drug Research

Proteomics in Drug Research
Author: Michael Hamacher
Publisher: John Wiley & Sons
Total Pages: 392
Release: 2006-03-31
Genre: Science
ISBN: 9783527312269

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From skillful handling of the wide range of technologies to successful applications in drug discovery -- this handbook has all the information professional proteomics users need. Edited by experts working at one of the hot spots in European proteomic research, the numerous contributions by experts from the pharmaceutical industry and public proteomics consortia to provide the necessary perspective on current trends and developments in this exciting field. Following an introductory chapter, the book moves on to proteomic technologies, such as protein biochips, protein-protein interactions, and proteome analysis in situ. The section on applications includes bioinformatics, Alzheimer's disease, neuroproteomics, plasma and T-cell proteomics, differential phosphoproteome analysis and biomarkers, as well as pharmacogenomics. Invaluable reading for medicinal and pharmaceutical chemists, gene technologists, molecular biologists, and those working in the pharmaceutical industry.