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Serology and Immunochemistry of Plant Viruses

Serology and Immunochemistry of Plant Viruses
Author: M.H.V. Van Regenmortel
Publisher: Elsevier
Total Pages: 317
Release: 2012-12-02
Genre: Science
ISBN: 0323156320

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Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses. It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical implementation of the various serological techniques. It also considers the problems associated with identification and classification of plant viruses. Organized into 10 chapters, this volume begins with an overview of antigens and antigenic determinants before proceeding with a discussion of the immunochemistry of plant viruses, virus-antibody binding, the role of quaternary structure in antigenicity, and the structure of viral antigenic determinants. The reader is also introduced to the methods and principles of purifying plant viruses, preparation of antisera and purification of antibodies, antigen-antibody interaction, immunochemical techniques used with plant viruses, the role of quaternary structure on viral antigenicity, diagnosis of virus diseases, use of serological criteria for measuring the degree of relationship between viruses, and immunochemical studies of plant viruses. The book includes a bibliography with 1,400 references and a list of all the plant viruses that have been studied by serology. This book will be a useful resource for virologists and plant pathologists, as well as for students and research workers in plant virology, plant pathology, microbiology, and general virology.


Plant Virus Serology

Plant Virus Serology
Author:
Publisher: CUP Archive
Total Pages: 160
Release:
Genre:
ISBN:

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The Plant Viruses

The Plant Viruses
Author: R.G. Milne
Publisher: Springer Science & Business Media
Total Pages: 431
Release: 2013-11-09
Genre: Science
ISBN: 1468470388

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The original aim of this book was to cover different aspects of the tradi tionally "filamentous" potex-, carla-, poty-, clostero-, and capilloviruses. The title The Filamentous Plant Viruses seemed the only suitable one, but it has led us to discuss also the quite different filamentous viruses of the rice stripe group-recently officially named the tenuivirus group which otherwise, indeed, might not have been conveniently covered in any volume of this series. The question must be asked: What is there new that justifies the presentation of a book of this kind? An outline of the answer may be Among the traditional filamentous viruses, much pro given as follows. gress has been made in elucidating the physical structure of potexvirus particles, and this work serves as an excellent model for discussion of and future experiments on the poty-, carla-, clostero-, and capilloviruses, which have comparable structures, although they are more difficult to manipulate. Work on the structure and strategy of the genomes of poty viruses is, however, relatively advanced and at a very interesting stage. The helper component that assists the aphid transmission of potyviruses has also recently received considerable attention, although the more we know about that, the less seems clear about the aphid transmission of the carlaviruses and closteroviruses, which apparently neither possess nor require a helper component.


Fundamentals of Plant Virology

Fundamentals of Plant Virology
Author: R C Matthews
Publisher: Academic Press
Total Pages: 416
Release: 2012-12-02
Genre: Science
ISBN: 0323138497

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Fundamentals of Plant Virology is an introductory student text covering all of modern plant virology. The author, Dr. R.E.F. Matthews, has written this coursebook based on his classic and comprehensive Plant Virology, Third Edition. Four introductory chapters review properties of viruses and cells and techniques used in their study. Five chapters are devoted to current knowledge of all major plant viruses and related pathogens. Seven chapters describe biological properties such as transmission, host response, disease, ecology, control, classification, and evolution of plant viruses. A historical and future overview concludes the text. Fundamentals of Plant Virology is a carefully designed instructional format for a plant virology course. It is also an invaluable resource for students of plant pathology and plant molecular biology. Summarizes knowledge on all aspects of plant virology Condenses all essential material from Plant Virology 3/e Compares basic properties of cells and viruses Outlines principles of gene manipulation technology Discusses serological techniques including monoclonal antibodies Geared to student level course


Diagnosis of Plant Virus Diseases

Diagnosis of Plant Virus Diseases
Author: R. E. F. Matthews
Publisher: CRC Press
Total Pages: 391
Release: 2017-12-08
Genre:
ISBN: 9781315892252

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Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.


Characterization of Plant Viruses

Characterization of Plant Viruses
Author: Alangar Ishwara Bhat
Publisher: Humana
Total Pages: 552
Release: 2021-03-20
Genre: Science
ISBN: 9781071603369

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This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.


Plant Virus Serology

Plant Virus Serology
Author: Richard Ellis Ford Matthews
Publisher: CUP Archive
Total Pages: 158
Release: 1957
Genre: Plant Viruses
ISBN:

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A Textbook of Plant Virus Diseases

A Textbook of Plant Virus Diseases
Author: Kenneth M. Smith
Publisher: Elsevier
Total Pages: 695
Release: 2012-12-02
Genre: Technology & Engineering
ISBN: 0323162053

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A Textbook of Plant Virus Diseases deals with a discussion of different plant viruses, their properties, and the corresponding diseases these viruses cause. It includes a list of host plants of every virus arranged alphabetically. The book discusses the new types of disease vectors, such as root-infesting fungi, nematode worms, mites, and insects that are both biting and sucking. It also discusses the development of new techniques in electron microscopy that is used to characterize in detail the causal viruses of a number of plant virus diseases. Furthermore, it presents the development of plant virus serology, as well as the improved methods of virus isolation that allow the classification of viruses into related groups. The book includes a Bibliography and an Index of Synonyms that can be used in identifying given virus name. In addition, cross-references are included for searching a particular virus under the alternative names. This book will be invaluable to ecologists, environmentalists, agriculturalists, and other scientists interested in plant viruses. Biology students will also find this reference helpful.