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| Author | : Ellen M. Ball |
| Publisher | : |
| Total Pages | : 42 |
| Release | : 1974 |
| Genre | : Identification |
| ISBN | : |
| Author | : M.H.V. Van Regenmortel |
| Publisher | : Elsevier |
| Total Pages | : 317 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323156320 |
Serology and Immunochemistry of Plant Viruses investigates the antigenic properties of plant viruses. It looks at the practical aspects of plant virus serology, along with the molecular basis of viral antigenicity, antigenic determinants in proteins, the structure of antibodies, virus purification, antiserum production, and the theoretical principles and practical implementation of the various serological techniques. It also considers the problems associated with identification and classification of plant viruses. Organized into 10 chapters, this volume begins with an overview of antigens and antigenic determinants before proceeding with a discussion of the immunochemistry of plant viruses, virus-antibody binding, the role of quaternary structure in antigenicity, and the structure of viral antigenic determinants. The reader is also introduced to the methods and principles of purifying plant viruses, preparation of antisera and purification of antibodies, antigen-antibody interaction, immunochemical techniques used with plant viruses, the role of quaternary structure on viral antigenicity, diagnosis of virus diseases, use of serological criteria for measuring the degree of relationship between viruses, and immunochemical studies of plant viruses. The book includes a bibliography with 1,400 references and a list of all the plant viruses that have been studied by serology. This book will be a useful resource for virologists and plant pathologists, as well as for students and research workers in plant virology, plant pathology, microbiology, and general virology.
| Author | : Ellen M. Ball |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 1974 |
| Genre | : |
| ISBN | : |
| Author | : R. E. F. Matthews |
| Publisher | : CRC Press |
| Total Pages | : 392 |
| Release | : 2019-07-23 |
| Genre | : Science |
| ISBN | : 1351079808 |
Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
| Author | : Moslih Al-Moslih |
| Publisher | : BoD – Books on Demand |
| Total Pages | : 174 |
| Release | : 2012-03-21 |
| Genre | : Medical |
| ISBN | : 9535103709 |
This book explains the concept of serological methods used in laboratory diagnoses of certain bacteria, mycoplasmas, viruses in humans, animals and plants, certain parasitic agents as well as autoimmune disease. The authors present up-to-date information concerning the serological methods in laboratory diagnosis of such infectious diseases. Section one deals with the serological methods for bacteria. Section 2 deals with serological methods in human, animal and plant viruses. Section 3 is concerned with the serological laboratory diagnosis of echinococcus and human toxocariasis agents. The last section deals with serological laboratory methods in the diagnosis of coeliac disease.
| Author | : R. E. F. Matthews |
| Publisher | : CRC Press |
| Total Pages | : 400 |
| Release | : 1993-08-03 |
| Genre | : Science |
| ISBN | : |
Overview; Host plants in diagnosis; Experimental transmission of viruses in diagnosis; Virus transmission through soil and by soil-inhabiting; Organisms in diagnosis; Inclusions in diagnosing plant virus diseases; Virus purification in relation to diagnosis; Serological procedures; Electron microscopy of in vitro preparations; Nucleic acid hybridization procedures; dsRNA in diagnosis; Diagnostic methods applicable no viroids; Strategies for implicating virus-like pathogens as the caused of diseases of unknown etiology; Complexes of transmission-dependent and helper viruses.
| Author | : K. Subramanya Sastry |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 343 |
| Release | : 2013-01-05 |
| Genre | : Medical |
| ISBN | : 8132208137 |
Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.
| Author | : |
| Publisher | : CUP Archive |
| Total Pages | : 160 |
| Release | : |
| Genre | : |
| ISBN | : |
| Author | : Alangar Ishwara Bhat |
| Publisher | : Humana |
| Total Pages | : 552 |
| Release | : 2021-03-20 |
| Genre | : Science |
| ISBN | : 9781071603369 |
This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.
| Author | : S. K. Raj |
| Publisher | : Springer Nature |
| Total Pages | : 402 |
| Release | : 2021-11-13 |
| Genre | : Science |
| ISBN | : 9811639191 |
This edited book elucidates the evolution of plant virus, genomic structure, diversity, plant-virus interaction, subcellular movement etc. The book reviews the biological machineries which allow the emergence of virus populations adapted by plant. The main objective of this book is the demonstration of a clear synergistic effect of plant viruses, an effect that was unexpectedly as important as applied alone. Ornamental plants are very popular and economically important worldwide. The international market of ornamentals is constantly expanding. Viruses and viroids can significantly reduce both decorative value and quality of propagated material of ornamentals. Due to the wide range of ornamental plant species and cultivars and their wide geographical distribution, the diversity of viruses that infect them is also high. The new emerging viruses are the causal agent for the economic loss of many important ornamental plants. Therefore, this book also adds value to current knowledge of virus stress response in ornamental plants and will provide the groundwork necessary for building future strategies for product enhancement. This book is of interest to teachers, researchers, capacity builders and policymakers. It can serve as additional reading material for undergraduate and graduate students of virology, agriculture and plant sciences.
