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RNA Turnover in Bacteria, Archaea and Organelles

RNA Turnover in Bacteria, Archaea and Organelles
Author: Lynne E. Maquat
Publisher: Academic Press
Total Pages: 621
Release: 2008-12-23
Genre: Science
ISBN: 0080922066

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Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the difference in processing of mRNA between eukaryotes, bacteria and archea. Benefit: Processing of mRNA differs greatly between eukaryotes, bacteria and archea and this affords researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover which are central to the process of gene expression. Benefit: Keeps MIE buyers and online subscribers up-to-date with the latest research Offers step by step lab instructions including necessary equipment and reagents. Benefit: Provides tried and tested techniques which eliminate searching through many different sources. Tested techniques are trustworthy and avoid pitfalls so the same mistakes are not made over and over


RNA Turnover in Bacteria, Archaea and Organelles

RNA Turnover in Bacteria, Archaea and Organelles
Author: Lynne E. Maquat
Publisher: Academic Press
Total Pages: 0
Release: 2008-12-19
Genre: Science
ISBN: 9780123743770

Download RNA Turnover in Bacteria, Archaea and Organelles Book in PDF, ePub and Kindle

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements, responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.


Natural Product Biosynthesis by Microorganisms and Plants

Natural Product Biosynthesis by Microorganisms and Plants
Author: D. A. Hopwood
Publisher: Academic Press
Total Pages: 523
Release: 2012
Genre: Alkaloids
ISBN: 0124046347

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The third of 3 volumes covering Natural product biosynthesis by microorganisms and plants. This new volume continues the legacy of this premier serial contains quality chapters authored by leaders in the field. The third of 3 volumes, it has chapters on such topics as metabolic pathways in Aspergillus oryzae, heterologous gene clusters and cyanobacteria as a source of natural products.


Microbial Metagenomics, Metatranscriptomics, and Metaproteomics

Microbial Metagenomics, Metatranscriptomics, and Metaproteomics
Author:
Publisher: Academic Press
Total Pages: 683
Release: 2013-09-19
Genre: Science
ISBN: 012407927X

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers microbial metagenomics, metatranscriptomics, and metaproteomics Contains chapters on such topics as in-solution fluorescence in situ hybridization (FISH) for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton


Natural Product Biosynthesis by Microorganisms and Plants Part C

Natural Product Biosynthesis by Microorganisms and Plants Part C
Author:
Publisher: Academic Press
Total Pages: 523
Release: 2012-12-31
Genre: Science
ISBN: 0124046177

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The third of 3 volumes covering Natural product biosynthesis by microorganisms and plants. This new volume continues the legacy of this premier serial Contains quality chapters authored by leaders in the field The third of 3 volumes, it has chapters on such topics as metabolic pathways in Aspergillus oryzae, heterologous gene clusters and cyanobacteria as a source of natural products


Complex Enzymes in Microbial Natural Product Biosynthesis, Part B: Polyketides, Aminocoumarins and Carbohydrates

Complex Enzymes in Microbial Natural Product Biosynthesis, Part B: Polyketides, Aminocoumarins and Carbohydrates
Author:
Publisher: Academic Press
Total Pages: 644
Release: 2009-04-11
Genre: Science
ISBN: 0080923364

Download Complex Enzymes in Microbial Natural Product Biosynthesis, Part B: Polyketides, Aminocoumarins and Carbohydrates Book in PDF, ePub and Kindle

Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. Gathers tried and tested methods and techniques from top players in the field Provides an extremely useful reference for the experienced research scientist Covers biosynthesis of Polyketides, Tarpenoids, Aminocoumarins and Crabohydrates


Complex Enzymes in Microbial Natural Product Biosynthesis, Part A: Overview Articles and Peptides

Complex Enzymes in Microbial Natural Product Biosynthesis, Part A: Overview Articles and Peptides
Author:
Publisher: Academic Press
Total Pages: 703
Release: 2009-04-10
Genre: Medical
ISBN: 0080923356

Download Complex Enzymes in Microbial Natural Product Biosynthesis, Part A: Overview Articles and Peptides Book in PDF, ePub and Kindle

Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. Gathers tried and tested methods and techniques from top players in the field In depth coverage of ribosomally-synthesised and Non-ribosomally-synthesised peptides Provides an extremely useful reference for the experienced research scientist


G Protein Coupled Receptors

G Protein Coupled Receptors
Author:
Publisher: Academic Press
Total Pages: 451
Release: 2013-01-17
Genre: Science
ISBN: 0123918723

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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers G protein coupled receptors Contains chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain


Guide to Yeast Genetics and Molecular Biology

Guide to Yeast Genetics and Molecular Biology
Author:
Publisher: Gulf Professional Publishing
Total Pages: 961
Release: 2004-05-14
Genre: Science
ISBN: 0080575137

Download Guide to Yeast Genetics and Molecular Biology Book in PDF, ePub and Kindle

Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein


Laboratory Methods in Enzymology: DNA

Laboratory Methods in Enzymology: DNA
Author:
Publisher: Elsevier
Total Pages: 405
Release: 2013-09-02
Genre: Science
ISBN: 0124199542

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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA