Rna Interference PDF Download
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Author | : R. K. Gaur |
Publisher | : CRC Press |
Total Pages | : 0 |
Release | : 2011-04-28 |
Genre | : Science |
ISBN | : 9781578087167 |
Download RNAi Technology Book in PDF, ePub and Kindle
RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA interference in microorganisms, plants, and animals, this book reviews the selective and robust effect of RNAi on gene expression. This process is essential both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest.
Author | : Muhammad Sohail |
Publisher | : CRC Press |
Total Pages | : 398 |
Release | : 2004-08-27 |
Genre | : Medical |
ISBN | : 020348925X |
Download Gene Silencing by RNA Interference Book in PDF, ePub and Kindle
Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r
Author | : Dr. Tim Doran |
Publisher | : CABI |
Total Pages | : 269 |
Release | : 2009 |
Genre | : Science |
ISBN | : 1845934105 |
Download RNA Interference Book in PDF, ePub and Kindle
This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.
Author | : Ute Schepers |
Publisher | : John Wiley & Sons |
Total Pages | : 336 |
Release | : 2006-03-06 |
Genre | : Science |
ISBN | : 3527604375 |
Download RNA Interference in Practice Book in PDF, ePub and Kindle
This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
Author | : Ibrokhim Y. Abdurakhmonov |
Publisher | : BoD – Books on Demand |
Total Pages | : 460 |
Release | : 2016-04-06 |
Genre | : Medical |
ISBN | : 953512272X |
Download RNA Interference Book in PDF, ePub and Kindle
RNA interference (RNAi), a hallmark of all biological sciences of twenty-first century, is an evolutionarily conserved and double-stranded RNA-dependent eukaryotic cell defense process. Opportunity to utilize an organisms own gene and to systematically induce and trigger RNAi for any desired sequence made RNAi an efficient approach for functional genomics, providing a solution for conventional longstanding obstacles in life sciences. RNAi research and application have significantly advanced during past two decades. This book RNA interference provides an updated knowledge and progress on RNAi in various organisms, explaining basic principles, types, and property of inducers, structural modifications, delivery systems/methodologies, and various successful bench-to-field or clinic applications and disease therapies with some aspects of limitations, alternative tools, safety, and risk assessment.
Author | : Wiley |
Publisher | : John Wiley & Sons |
Total Pages | : 214 |
Release | : 2013-11-26 |
Genre | : Science |
ISBN | : 1118829441 |
Download RNA Interference (RNAi) Book in PDF, ePub and Kindle
The 2006 Nobel Prize in Physiology or Medicine was awarded to Andrew Z. Fire and Craig C. Mello for their discovery of RNA interference (RNAi). RNAi is a biological process in which RNA molecules can silence (inhibit) or up- or down-regulate gene expression, typically by causing the destruction of specific messenger RNA (mRNA) molecules. This collection of articles begins with an introduction to RNAi, and proceeds to describe uses of this technology in various approaches to disease treatment, including gene therapy. Several laboratory protocols for silencing genes via RNAi are also provided, as are protocols for down-regulating and then rescuing those down-regulated genes, which demonstrates specificity of the approach. This e-book — a curated collection from eLS, WIREs, and Current Protocols — offers a fantastic introduction to the field of RNA interference for students or interdisciplinary collaborators.
Author | : Kenneth A. Howard |
Publisher | : Springer Science & Business Media |
Total Pages | : 348 |
Release | : 2012-10-19 |
Genre | : Medical |
ISBN | : 1461447437 |
Download RNA Interference from Biology to Therapeutics Book in PDF, ePub and Kindle
The enormous potential of siRNA as a therapeutic has led to an explosion of interest from the scientific community. There has been intense interest from Big Pharma to capitalise on this new technology but the fact remains that delivery is a key determinant in realizing the full clinical potential of RNA interference. There is an urgent need for better delivery methods to take this technology forward. This book addresses the role of different RNAi molecules in cellular processes as rational for diagnostic and therapeutic approaches. This book will cover RNAi therapeutic design to optimize siRNA potency and reduce off-target effects and current delivery technologies to overcome both intracellular and extracellular barriers. The reader will gain an insight into RNA interference from the cellular mechanisms to screening to siRNA design right through to diagnostic and therapeutic applications.
Author | : Krishnarao Appasani |
Publisher | : Cambridge University Press |
Total Pages | : 564 |
Release | : 2005-01-17 |
Genre | : Science |
ISBN | : 9781139442657 |
Download RNA Interference Technology Book in PDF, ePub and Kindle
RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this overview, the basic concepts and applications of RNAi biology are discussed. Leading experts from both academia and industry have contributed to this invaluable reference. The volume is forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference.
Author | : Dirk Grimm |
Publisher | : Academic Press |
Total Pages | : 264 |
Release | : 2011-08-26 |
Genre | : Medical |
ISBN | : 0124157955 |
Download Cellular RNA Interference Mechanisms Book in PDF, ePub and Kindle
This volume explores some of the most exciting recent advances in basic research on cellular RNA interference mechanisms and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and ideas Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields
Author | : Gregory J. Hannon |
Publisher | : CSHL Press |
Total Pages | : 568 |
Release | : 2003 |
Genre | : Gene silencing |
ISBN | : 9780879696412 |
Download RNAi Book in PDF, ePub and Kindle
In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.