Revealing Uncharted Biology With Single Cell Multiplex Proteomic Technologies PDF Download
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Author | : Wendy Fantl |
Publisher | : Academic Press |
Total Pages | : 204 |
Release | : 2024-06-28 |
Genre | : Science |
ISBN | : 012822214X |
Download Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies Book in PDF, ePub and Kindle
Revealing Unchartered Biology with Single Intact Cells: Case Studies explores the path to research success, key projects, the role of techniques, the selection process, other alternatives considered, what other paths have led to dead ends, detailed protocols followed, and how the analysis of generated data allowed researchers to visualize unchartered biology. Focusing on the research journey that led to the publication of each article, the book's editors interviewed the researchers on the use of the Multiplex Single Cell technique and how it helped hone in on the biological quest. These methods can be expanded to a wide variety of research objectives. In conclusion to each chapter, the authors critically review their process and provide suggestions of improvement or alternate techniques that could be employed. This book is the ideal reference for researchers new to the world of single-cell multiplex techniques. The discussion on failures encountered along the research path provides insights on how to avoid repeating the same errors. Provides insights into the path to success of key research articles based on Multiplex Single-Cell analysis techniques results Contains detailed method information Discusses strengths and limitations of techniques applied to each research domain covered Includes discussions on the failures encountered along the research path and how to avoid them
Author | : Wendy Fantl |
Publisher | : Elsevier |
Total Pages | : 238 |
Release | : 2024-06-28 |
Genre | : Science |
ISBN | : 0128222093 |
Download Revealing Uncharted Biology with Single Cell Multiplex Proteomic Technologies Book in PDF, ePub and Kindle
Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms enables researchers in bioscience to visualize cellular phenomena at an unprecedented scale and rate. The book's editors provide an overview of each of the most current techniques, both individually and when used in conjunction. Sections cover CyTof, Multicolor fluorescence cytometry, CODEX multiplexed imaging, MIBI, Imaging Mass Cytometry, Multi resolution cell orientation, Confocal microscopy, QPI and Fluorescence microscopy, CyTOF and SCRNA Seq, CyTOP and scATAC Seq, and CyTOF and ATAC See, and the application of the techniques to biological questions, including chapters on cellular diversity and generating hypothesis through data analysis. The final part is dedicated to further the reader's understanding of complex biological systems by identifying new clinical tools and drug development. This book is the ideal reference for researchers who want to understand the variety of multiplex visualization techniques available for bioscientists and how to make better decisions on how to apply them to the biological question of interest.
Author | : Aleksei Anatoliyovych Stepanenko |
Publisher | : Delve Publishing |
Total Pages | : 0 |
Release | : 2017-11 |
Genre | : Cytology |
ISBN | : 9781773610306 |
Download Single-Cell Research Book in PDF, ePub and Kindle
Analysis of single cells has become a key technique in biological and biomedical research. Single-cell genomics, transcriptomics, epigenomics, and proteomics now allow many enduring questions in biological and biomedical sciences to be answered. The new single-cell approaches offer the potential to revolutionize our understanding of diseases and associated biological processes, with the capacity to reveal the intercellular heterogeneity within a specific tissue at an unprecedented resolution. The single cell technology has proven to be a valuable tool to explore DNA, RNA, or proteins in one single cell at a time. Using single-cell level features, we can infer cell lineages, identify subpopulations and highlight cell-specific biological characteristics. Moreover, single-cell analyses have already demonstrated their utilities in the clinical applications, ranging from characterizing cancer cells subpopulations, highlighting specific resistance mechanisms to being used as diagnostic tools. For example, by employing single-cell sequencing to circulating tumor cells, metastasis and progression diagnoses may aid in therapeutic design. Single-cell sequencing analyses have been applied in the field of virology, providing new ways to explore viral diversity and cell response to viral infection. Single-cell RNA sequencing have been used to dissect plant tissue organization, developmental dynamics, and physiological responses. Single-cell analysis have made increasingly significant contributions to our understanding of the role that somatic genome variations play in neuronal diversity and behaviors In this book, the technologies recently developed for single cell isolation, genome acquisition, transcriptome, and proteome analyses, their applications and the future potentials are thoroughly discussed.
Author | : Valentina Proserpio |
Publisher | : |
Total Pages | : 459 |
Release | : 2019 |
Genre | : Cytology |
ISBN | : 9781493992409 |
Download Single Cell Methods Book in PDF, ePub and Kindle
Author | : Valentina Proserpio |
Publisher | : |
Total Pages | : 452 |
Release | : 2019 |
Genre | : Cytology |
ISBN | : 9781493992416 |
Download Single Cell Methods Book in PDF, ePub and Kindle
This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Cell Methods: Sequencing and Proteomics aims to make each experiment easily reproducible in every lab.
Author | : Mihail C. Roco |
Publisher | : Springer Science & Business Media |
Total Pages | : 477 |
Release | : 2013-04-17 |
Genre | : Technology & Engineering |
ISBN | : 9401703590 |
Download Converging Technologies for Improving Human Performance Book in PDF, ePub and Kindle
M. C. Roco and W.S. Bainbridge In the early decades of the 21st century, concentrated efforts can unify science based on the unity of nature, thereby advancing the combination of nanotechnology, biotechnology, information technology, and new technologies based in cognitive science. With proper attention to ethical issues and societal needs, converging in human abilities, societal technologies could achieve a tremendous improvement outcomes, the nation's productivity, and the quality of life. This is a broad, cross cutting, emerging and timely opportunity of interest to individuals, society and humanity in the long term. The phrase "convergent technologies" refers to the synergistic combination of four major "NBIC" (nano-bio-info-cogno) provinces of science and technology, each of which is currently progressing at a rapid rate: (a) nanoscience and nanotechnology; (b) biotechnology and biomedicine, including genetic engineering; (c) information technology, including advanced computing and communications; (d) cognitive science, including cognitive neuroscience. Timely and Broad Opportunity. Convergence of diverse technologies is based on material unity at the nanoscale and on technology integration from that scale.
Author | : Tim D. Hewitson |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2009-12-16 |
Genre | : Science |
ISBN | : 9781603273442 |
Download Histology Protocols Book in PDF, ePub and Kindle
Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols.
Author | : Institute of Medicine |
Publisher | : National Academies Press |
Total Pages | : 358 |
Release | : 2008-03-12 |
Genre | : Medical |
ISBN | : 0309108810 |
Download Autism and the Environment Book in PDF, ePub and Kindle
Autism spectrum disorders (ASD) constitute a major public health problem, affecting one in every 150 children and their families. Unfortunately, there is little understanding of the causes of ASD, and, despite their broad societal impact, many people believe that the overall research program for autism is incomplete, particularly as it relates to the role of environmental factors. The Institute of Medicine's Forum on Neuroscience and Nervous System Disorders, in response to a request from the U.S. Secretary of Health and Human Services, hosted a workshop called "Autism and the Environment: Challenges and Opportunities for Research." The focus was on improving the understanding of the ways in which environmental factors such as chemicals, infectious agents, or physiological or psychological stress can affect the development of the brain. Autism and the Environment documents the concerted effort which brought together the key public and private stakeholders to discuss potential ways to improve the understanding of the ways that environmental factors may affect ASD. The presentations and discussions from the workshop that are described in this book identify a number of promising directions for research on the possible role of different environmental agents in the etiology of autism.
Author | : Zachary Davis |
Publisher | : |
Total Pages | : 580 |
Release | : 2021-01-30 |
Genre | : |
ISBN | : 9781952565076 |
Download Strategic Latency Unleashed Book in PDF, ePub and Kindle
The world is being transformed physically and politically. Technology is the handmaiden of much of this change. But since the current sweep of global change is transforming the face of warfare, Special Operations Forces (SOF) must adapt to these circumstances. Fortunately, adaptation is in the SOF DNA. This book examines the changes affecting SOF and offers possible solutions to the complexities that are challenging many long-held assumptions. The chapters explore what has changed, what stays the same, and what it all means for U.S. SOF. The authors are a mix of leading experts in technology, business, policy, intelligence, and geopolitics, partnered with experienced special operators who either cowrote the chapters or reviewed them to ensure accuracy and relevance for SOF. Our goal is to provide insights into the changes around us and generate ideas about how SOF can adapt and succeed in the emerging operational environment.
Author | : Sandy B. Primrose |
Publisher | : John Wiley & Sons |
Total Pages | : 232 |
Release | : 2008-04-15 |
Genre | : Science |
ISBN | : 0470751274 |
Download Genomics Book in PDF, ePub and Kindle
Written by the successful author team of Sandy Primrose and Richard Twyman, Genomics: Applications in Human Biology is a topical book showing how the new science of genomics is adding impetus to the advances in human health provided by biotechnology. Written to provide the necessary overview of the subject, covering technological developments, applications and (where necessary) the ethical implications. Divided into three sections, the first section introduces the role of biotechnology and genomics in medicine and sets out some of the technological advances that have been the basis of recent medical breakthroughs. The second section takes a closer look at how biotechnology and genomics are influencing the prevention and treatment of different categories of disease. Finally the contribution of biotechnology and genomics to the development of different types of therapy is described, including conventional drugs, recombinant proteins and gene/cell therapies. References to appropriate sections in other two popular books, authored by Sandy Primrose and Richard Twyman, are included - Principles of Gene Manipulation and Principles of Gene Analysis and Genomics. Features several categories of boxed text, including history boxes (describing the origins and development of particular technologies or treatments), molecular boxes (featuring the molecular basis of diseases or treatments in more detail) and ethic boxes (which discusses the ethical implications of technology development and new therapies).