Retention And Selectivity In Liquid Chromatography PDF Download

Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Retention And Selectivity In Liquid Chromatography PDF full book. Access full book title Retention And Selectivity In Liquid Chromatography.

Retention and Selectivity in Liquid Chromatography

Retention and Selectivity in Liquid Chromatography
Author: R.M. Smith
Publisher: Elsevier
Total Pages: 456
Release: 1995-01-13
Genre: Science
ISBN: 9780080858654
GET BOOK

Download Retention and Selectivity in Liquid Chromatography Book in PDF, ePub and Kindle

This book brings together a number of studies which examine the ways in which the retention and selectivity of separations in high-performance liquid chromatography are dependent on the chemical structure of the analytes and the properties of the stationary and mobile phases. Although previous authors have described the optimisation of separations by alteration of the mobile phase, little emphasis has previously been reported of the influence of the structure and properties of the analyte. The initial chapters describe methods based on retention index group increments and log P increments for the prediction of the retention of analytes and the ways in which these factors are influenced by mobile phases and intramolecular interactions. The values of a wide range of group increments in different eluents are tabulated. Different scales of retention indices in liquid chromatography are described for the comparison of separations, the identification of analytes and the comparison of stationary phases. Applications of these methods in the pharmaceutical, toxicology, forensic, metabolism, environmental, food and other fields are reviewed. The effects of different mobile phases on the selectivity of the retention indices are reported. A compilation of sources of reported retention index values are given. Methods for the comparison of stationary phases based on the interactions of different analytes are covered, including lipophilic and polar indices, shape selectivity comparisons, their application to novel stationary phases, and chemometric methods for column comparisons.

Introduction to Modern Liquid Chromatography

Introduction to Modern Liquid Chromatography
Author: Lloyd R. Snyder
Publisher: John Wiley & Sons
Total Pages: 831
Release: 2011-09-20
Genre: Science
ISBN: 1118210395
GET BOOK

Download Introduction to Modern Liquid Chromatography Book in PDF, ePub and Kindle

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

High-Performance Liquid Chromatography

High-Performance Liquid Chromatography
Author: Csaba Horváth
Publisher: Elsevier
Total Pages: 343
Release: 2013-10-22
Genre: Science
ISBN: 1483262022
GET BOOK

Download High-Performance Liquid Chromatography Book in PDF, ePub and Kindle

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.

Modern Practice of Gas Chromatography

Modern Practice of Gas Chromatography
Author: Robert L. Grob, PhD
Publisher: John Wiley & Sons
Total Pages: 1059
Release: 2004-08-04
Genre: Science
ISBN: 047165115X
GET BOOK

Download Modern Practice of Gas Chromatography Book in PDF, ePub and Kindle

The bible of gas chromatography-offering everything the professional and the novice need to know about running, maintaining, and interpreting the results from GC Analytical chemists, technicians, and scientists in allied disciplines have come to regard Modern Practice of Gas Chomatography as the standard reference in gas chromatography. In addition to serving as an invaluable reference for the experienced practitioner, this bestselling work provides the beginner with a solid understanding of gas chromatographic theory and basic techniques. This new Fourth Edition incorporates the most recent developments in the field, including entirely new chapters on gas chromatography/mass spectrometry (GC/MS); optimization of separations and computer assistance; high speed or fast gas chromatography; mobile phase requirements: gas system requirements and sample preparation techniques; qualitative and quantitative analysis by GC; updated information on detectors; validation and QA/QC of chromatographic methods; and useful hints for good gas chromatography. As in previous editions, contributing authors have been chosen for their expertise and active participation in their respective areas. Modern Practice of Gas Chromatography, Fourth Edition presents a well-rounded and comprehensive overview of the current state of this important technology, providing a practical reference that will greatly appeal to both experienced chomatographers and novices.

Liquid Chromatography

Liquid Chromatography
Author: G. Ramis-Ramos
Publisher: Elsevier Inc. Chapters
Total Pages: 520
Release: 2013-01-08
Genre: Science
ISBN: 0128071370
GET BOOK

Download Liquid Chromatography Book in PDF, ePub and Kindle

Many solvents can be used to prepare mobile phases in liquid chromatography. Also, mixtures of solvents at different ratios are used to modify the mobile phase properties. This can make solvent selection for method development a hard task, unless suitable guidelines are followed. This chapter summarizes the most common strategies used by skilled chromatographists in reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography. These are based on considerations about the global polarity of solutes, stationary phase and mobile phase, which determine the elution strength, and on the particular profile of the contributions of intermolecular interactions to the global polarity, which determines the selectivity. The optimization of selectivity using isoeluotropic mixtures on the rational basis provided by solvent selectivity triangles and systematic trial-and-error procedures in the isocratic and gradient elution modes is described. Additional considerations for mobile-phase selection, including computer-assisted interpretive optimization, sustainability criteria, and the use of high temperatures, are also commented.

Optimization of Chromatographic Selectivity

Optimization of Chromatographic Selectivity
Author: P.J. Schoenmakers
Publisher: Elsevier
Total Pages: 344
Release: 1986-08-01
Genre: Science
ISBN: 9780080858388
GET BOOK

Download Optimization of Chromatographic Selectivity Book in PDF, ePub and Kindle

This is the first detailed description of method development in chromatography - the overall process of which may be summarized as: method selection, phase selection, selectivity optimization, and system optimization. All four aspects receive attention in this book. Chapter 1 gives a short introduction, describes chromatographic theory and nomenclature, and outlines the method development process. Chapter 2 describes guidelines for method selection, and quantitative concepts for characterizing and classifying chromatographic phases. Selective separation methods, from both gas and liquid chromatography are given in Chapter 3; the main parameters of each method are identified and simple, quantitative relations are sought to describe their effects. Criteria by which to judge the quality of separation are discussed in Chapter 4 with clear recommendations for different situations. The specific problems involved in the optimization of chromatographic selectivity are explained in Chapter 5. Optimization procedures, illustrated by examples, are extensively described and compared on the basis of a number of criteria. Suggestions are made both for the application of different procedures and for further research. The optimization of programmed analysis receives special attention in Chapter 6, and the last chapter summarizes the optimization of the chromatographic system, including the optimization of the efficiency, sensitivity and instrumentation. Those involved in developing chromatographic methods or wishing to improve existing methods will value the detailed, structured way in which the subject is presented. Because optimization procedures and criteria are described as elements of a complete optimization package, the book will help the reader to understand, evaluate and select current and future commercial systems.

High Performance Liquid Chromatography

High Performance Liquid Chromatography
Author: W.J. Lough
Publisher: CRC Press
Total Pages: 292
Release: 1995-09-30
Genre: Science
ISBN: 9780751400762
GET BOOK

Download High Performance Liquid Chromatography Book in PDF, ePub and Kindle

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

High-Performance Gradient Elution

High-Performance Gradient Elution
Author: Lloyd R. Snyder
Publisher: John Wiley & Sons
Total Pages: 496
Release: 2007-01-09
Genre: Science
ISBN: 0470055510
GET BOOK

Download High-Performance Gradient Elution Book in PDF, ePub and Kindle

Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.

High Performance Liquid Chromatography

High Performance Liquid Chromatography
Author: Mary T. Gilbert
Publisher: Elsevier
Total Pages: 397
Release: 2013-10-22
Genre: Science
ISBN: 1483281299
GET BOOK

Download High Performance Liquid Chromatography Book in PDF, ePub and Kindle

High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Chiral Chromatography

Chiral Chromatography
Author: Thomas E. Beesley
Publisher: John Wiley & Sons
Total Pages: 526
Release: 1999-01-26
Genre: Science
ISBN: 9780471974277
GET BOOK

Download Chiral Chromatography Book in PDF, ePub and Kindle

Eine Ergänzung anderer, in der Reihe Separation Sciences bereits erschienener Titel. Viel Theorie und zahlreiche Anwendungsbeispiele für die Praxis der chiralen HPLC- und GC-Trennung machen dieses top-aktuelle Buch zu einer unverzichtbaren Informationsquelle für jeden Neueinsteiger auf diesem Gebiet. (11/98)