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Recombinant DNA Principles and Methodologies

Recombinant DNA Principles and Methodologies
Author: James Greene
Publisher: CRC Press
Total Pages: 768
Release: 2021-12-17
Genre: Science
ISBN: 1000105474

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This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.


Molecular Biotechnology

Molecular Biotechnology
Author: Bernard R. Glick
Publisher: John Wiley & Sons
Total Pages: 796
Release: 2017-06-01
Genre: Science
ISBN: 1683673107

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Since 1994, Molecular Biotechnology: Principles and Applications of Recombinant DNA has introduced students to the fast-changing world of molecular biotechnology. With each revision, the authors have extensively updated the book to keep pace with the many new techniques in gene isolation and amplification, nucleic acid synthesis and sequencing, gene editing, and their applications to biotechnology. In this edition, authors Bernard R. Glick and Cheryl L. Patten have continued that tradition, but have also overhauled the book's organization to Detail fundamental molecular biology methods and recombinant protein engineering techniques, which provides students with a solid scientific basis for the rest of the book. Present the processes of molecular biotechnology and its successes in medicine, bioremediation, raw material production, biofuels, and agriculture. Examine the intersection of molecular biotechnology and society, including regulation, patents, and controversies around genetically modified products. Filled with engaging figures that strongly support the explanations in the text, Molecular Biotechnology: Principles and Applications of Recombinant DNA presents difficult scientific concepts and technically challenging methods in clear, crisp prose. This excellent textbook is ideal for undergraduate and graduate courses in introductory biotechnology, as well as, courses dedicated to medical, agricultural, environmental, and industrial biotechnology applications.


Molecular Biotechnology

Molecular Biotechnology
Author: Bernard R. Glick
Publisher:
Total Pages: 724
Release: 1998
Genre: Medical
ISBN:

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The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.


Recombinant DNA Technology

Recombinant DNA Technology
Author: Sardul Singh Sandhu
Publisher: I. K. International Pvt Ltd
Total Pages: 227
Release: 2013-12-30
Genre: Science
ISBN: 938057844X

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Introduces the basic principles and techniques of recombinant DNA. The book begins with an introduction to the different tools used for gene cloning. The final chapters cover the application of Recombinant Technology to current research and provide an inside look at the human genome project, ribozyme technology, antisense technology, DNA sequencing, and protein engineering.


Recombinant DNA Methodology

Recombinant DNA Methodology
Author:
Publisher: Academic Press
Total Pages: 793
Release: 2014-05-19
Genre: Science
ISBN: 1483295974

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Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors


Enzymology Primer for Recombinant DNA Technology

Enzymology Primer for Recombinant DNA Technology
Author: Hyone-Myong Eun
Publisher: Elsevier
Total Pages: 727
Release: 1996-06-03
Genre: Science
ISBN: 008053113X

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Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques


Recombinant DNA Technology

Recombinant DNA Technology
Author: Keya Chaudhuri
Publisher: The Energy and Resources Institute (TERI)
Total Pages: 300
Release: 2013-01-01
Genre: Science
ISBN: 8179933202

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Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.


Recombinant DNA Technology

Recombinant DNA Technology
Author: Siddra Ijaz
Publisher: Cambridge Scholars Publishing
Total Pages: 156
Release: 2019-08-13
Genre: Science
ISBN: 1527538443

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Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.


Principles of Gene Manipulation and Genomics

Principles of Gene Manipulation and Genomics
Author: Sandy B. Primrose
Publisher: Wiley-Blackwell
Total Pages: 704
Release: 2007
Genre: Science
ISBN: 9781405156660

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Now in its eighth edition, Principles of Gene Manipulation and Genomics embraces the burgeoning revolution in recombinant DNA technology and its applications. Providing integrated coverage of the techniques used for gene manipulation, genomics, and its related disciplines, the text features full-color illustrations throughout. Chapter summaries and thought-provoking end-of-chapter questions plus a dedicated website provides further instruction and resources for both the student and instructor as well as regular updates on important topics elucidate learning for undergraduate and graduate courses in genetics, genomics, genome analysis, and gene cloning understanding.