Protein Engineering Protocols PDF Download
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Author | : Kristian Müller |
Publisher | : Springer Science & Business Media |
Total Pages | : 318 |
Release | : 2007-10-26 |
Genre | : Science |
ISBN | : 1597451878 |
Download Protein Engineering Protocols Book in PDF, ePub and Kindle
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.
Author | : Peter W. Goodenough |
Publisher | : |
Total Pages | : 400 |
Release | : 1998-10 |
Genre | : |
ISBN | : 9780896035676 |
Download Protein Engineering Methods and Protocols Book in PDF, ePub and Kindle
Author | : Uwe T. Bornscheuer |
Publisher | : Humana Press |
Total Pages | : 350 |
Release | : 2017-10-30 |
Genre | : Science |
ISBN | : 9781493973644 |
Download Protein Engineering Book in PDF, ePub and Kindle
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
Author | : Victor Muñoz |
Publisher | : Humana |
Total Pages | : 419 |
Release | : 2021-11-30 |
Genre | : Science |
ISBN | : 9781071617151 |
Download Protein Folding Book in PDF, ePub and Kindle
This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Author | : |
Publisher | : Academic Press |
Total Pages | : 539 |
Release | : 2013-02-14 |
Genre | : Science |
ISBN | : 0123946298 |
Download Methods in Protein Design Book in PDF, ePub and Kindle
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers methods in protein design and it has chapters on such topics as protein switch engineering by domain insertion, evolution based design of proteins, and computationally designed proteins. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers methods in protein design Contains chapters with such topics as protein switch engineering by domain insertion, evolution-based design of proteins, and computationally designed proteins
Author | : James Samuelson |
Publisher | : Humana Press |
Total Pages | : 266 |
Release | : 2016-05-01 |
Genre | : Science |
ISBN | : 9781493962877 |
Download Enzyme Engineering Book in PDF, ePub and Kindle
This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.
Author | : Yawen Bai |
Publisher | : Springer Science & Business Media |
Total Pages | : 332 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 1597451894 |
Download Protein Folding Protocols Book in PDF, ePub and Kindle
Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a broad range of techniques to study, predict, and analyze the protein folding process. Protein Folding Protocols also provides sample approaches toward the prediction of protein structure starting from the amino acid sequence, in the absence of overall homologous sequences.
Author | : Gregory L. Moore |
Publisher | : |
Total Pages | : 195 |
Release | : 2005 |
Genre | : |
ISBN | : 9780549002482 |
Download Modeling and Optimization in Directed Evolution Protocols and Protein Engineering Book in PDF, ePub and Kindle
The central theme of this thesis aims toward the systematic development of integrated approaches for proactively designing protein libraries with focused diversity for directed evolution and protein engineering experiments. Experimental paradigms for library generation rely on the generation of point mutations, recombination of parental sequence segments, and ab initio library design using degenerate oligonucleotides. In response to these library design paradigms, this thesis presents a computational toolbox for quantifying how diversity is generated and allocated in the combinatorial DNA library and what sequence permutations are the most promising in terms of preserving protein structure and activity.
Author | : K. Dane Wittrup |
Publisher | : Academic Press |
Total Pages | : 399 |
Release | : 2012 |
Genre | : Electronic books |
ISBN | : 012396962X |
Download Protein Engineering for Therapeutics Book in PDF, ePub and Kindle
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike. Iinternational board of authors. This volume is split into sections that cover subjects such as Peptides, and Scaffolds
Author | : Dan Robertson |
Publisher | : Elsevier |
Total Pages | : 453 |
Release | : 2004-09-29 |
Genre | : Science |
ISBN | : 008049725X |
Download Protein Engineering Book in PDF, ePub and Kindle
This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies. Table of Contents-Methodology-Applications-Opzimization and Screening-Applications-Directed Evolution of Enzymatic Function-Applications-Evolution of Biosynthetic Pathways-Devices, Antibodies and Vaccines