Precise Optical Manipulation Techniques For Exploring Neuronal Function PDF Download
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Author | : Mary Ann Go |
Publisher | : |
Total Pages | : 0 |
Release | : 2014 |
Genre | : |
ISBN | : |
Download Precise Optical Manipulation Techniques for Exploring Neuronal Function Book in PDF, ePub and Kindle
This thesis presents two optical techniques for manipulating neurons in the analysis of their function, namely time-gated holographic two-photon photostimulation and femtosecond laser dendrotomy. At the start of this thesis, no illumination technique had been able to activate synapses along the three-dimensional (3D) dendritic arbour of a neuron using realistic spatio-temporal patterns of light that resemble physiological stimuli, a prerequisite to a systematic study of synaptic integration. This thesis demonstrates that holographic projection with a spatial light modulator (SLM) enables the generation of multiple uncaging focal spots at arbitrary locations in 3D and allows full control over the spatial organisation of uncaging sites. The incorporation of a digital micromirror device (DMD) in the setup allows high-speed independent switching of individual focal spots, enabling control over the temporal organisation of uncaging events. Together, the SLM-DMD combination offers unprecedented flexibility in the design and generation of spatio-temporal patterns of synaptic input for integration studies. A technique for optimising the holographic projection technique used for photostimulation is also presented. The technique incorporates a generalised phase contrast (GPC) setup into a typical SLM holographic setup to generate a complex field hologram, as opposed to conventional phase-only holograms. Numerical simulations show that the technique produces higher optical throughput for the projection of three or more foci. It also significantly decreases the intensity of unwanted higher diffraction orders, providing cleaner excitation patterns. This technique has the potential to increase the number of holographic photostimulation foci achievable with current techniques. Finally, this thesis demonstrates femtosecond laser dendrotomy as a precise and minimally-invasive way to prune dendrites at different locations in the tree. Its impact on the firing frequency of the neuron is evaluated. This technique has been used emph{in vivo} but neuronal properties during and after dendrotomy have not been previously measured. This study found that small-scale dendrotomy has negligible effect on the input resistance or the firing pattern of the neuron. However, large-scale dendrotomy significantly increases the input resistance of the neuron and its excitability. This technique offers the capability to experimentally manipulate dendritic morphology for the study of neuronal structure-function relationship.
Author | : Eirini Papagiakoumou |
Publisher | : Springer Nature |
Total Pages | : 424 |
Release | : 2023-02-20 |
Genre | : Medical |
ISBN | : 1071627643 |
Download All-Optical Methods to Study Neuronal Function Book in PDF, ePub and Kindle
This open access volume provides an overview of the latest methods used to study neuronal function with all-optical experimental approaches, where light is used for both stimulation and monitoring of neuronal activity. The chapters in this book cover topics over a broad range, from fundamental background information in both physiology and optics in the context of all-optical neurophysiology experiments, to the design principles and hardware implementation of optical methods used for photoactivation and imaging. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, All-Optical Methods to Study Neuronal Function is a valuable resource for researchers in various disciplines such as physics, engineering, and neuroscience. This book will serve as a guide to establish useful references for groups starting out in this field, and provide insight on the optical systems, actuators, and sensors. This is an open access book.
Author | : Shengxiang Liang |
Publisher | : Frontiers Media SA |
Total Pages | : 128 |
Release | : 2023-03-10 |
Genre | : Science |
ISBN | : 2832517137 |
Download Image Processing Methods in Animal MRI and their Application to Evaluate Brain Function Book in PDF, ePub and Kindle
Author | : Srdjan D. Antic |
Publisher | : Frontiers Media SA |
Total Pages | : 193 |
Release | : 2020-01-06 |
Genre | : |
ISBN | : 2889632679 |
Download New Insights on Neuron and Astrocyte Function from Cutting-Edge Optical Techniques Book in PDF, ePub and Kindle
Author | : M. Abeles |
Publisher | : Cambridge University Press |
Total Pages | : 298 |
Release | : 1991-02-22 |
Genre | : Science |
ISBN | : 9780521376174 |
Download Corticonics Book in PDF, ePub and Kindle
Understanding how the brain works is probably the greatest scientific and intellectual challenge of our generation. The cerebral cortex is the instrument by which we carry the most complex mental functions. Fortunately, there exists an immense body of knowledge concerning both cortical structure and the properties of single neurons in the cortex. With the advent of the supercomputer, there has been increased interest in neural network modeling. What is needed is a new approach to an understanding of the mammalian cerebral cortex that will provide a link between the physiological description and the computer model. This book meets that need by combining anatomy, physiology, and modeling to achieve a quantitative description of cortical function. The material is presented didactically, starting with descriptive anatomy and comprehensively examining all aspects of modeling. The book gradually leads the reader from the macroscopic cortical anatomy and standard electrophysiological properties of single neurons to neural network models and synfire chains. The most modern trends in neural network modeling are explored.
Author | : International Biomedical Optics Society |
Publisher | : SPIE-International Society for Optical Engineering |
Total Pages | : 152 |
Release | : 1997 |
Genre | : Science |
ISBN | : |
Download Proceedings of Functional Imaging and Optical Manipulation of Living Cells Book in PDF, ePub and Kindle
Author | : Matt Carter |
Publisher | : Academic Press |
Total Pages | : 416 |
Release | : 2022-03-26 |
Genre | : Medical |
ISBN | : 0323915612 |
Download Guide to Research Techniques in Neuroscience Book in PDF, ePub and Kindle
Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step
Author | : Albrecht Stroh |
Publisher | : Humana Press |
Total Pages | : 302 |
Release | : 2017-10-25 |
Genre | : Medical |
ISBN | : 9781493974153 |
Download Optogenetics: A Roadmap Book in PDF, ePub and Kindle
This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.
Author | : Michael W. Berns |
Publisher | : Frontiers Media SA |
Total Pages | : 362 |
Release | : 2022-02-03 |
Genre | : Science |
ISBN | : 2889742431 |
Download Optical Trapping (Laser Tweezers) and Nanosurgery (Laser Scissors) Book in PDF, ePub and Kindle
Author | : Gerard Marriott |
Publisher | : Academic Press |
Total Pages | : 584 |
Release | : 1998-06-22 |
Genre | : Medical |
ISBN | : |
Download Caged Compounds Book in PDF, ePub and Kindle
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.