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Plant Molecular Biology — A Laboratory Manual

Plant Molecular Biology — A Laboratory Manual
Author: Melody S. Clark
Publisher: Springer Science & Business Media
Total Pages: 551
Release: 2013-11-27
Genre: Science
ISBN: 3642878733

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Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.


Plant Biotechnology and Molecular Biology : A Laboratory Manual

Plant Biotechnology and Molecular Biology : A Laboratory Manual
Author: M.S. Punia
Publisher: Scientific Publishers
Total Pages: 255
Release: 2018-01-01
Genre: Technology & Engineering
ISBN: 9388172361

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The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols.


Plant Molecular Biology Manual

Plant Molecular Biology Manual
Author: S.B. Gelvin
Publisher: Springer Science & Business Media
Total Pages: 180
Release: 2013-03-09
Genre: Science
ISBN: 9401142173

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Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.


Plant Molecular Biology Manual

Plant Molecular Biology Manual
Author: S. B. Gelvin
Publisher: Springer
Total Pages: 111
Release: 2013-06-29
Genre: Science
ISBN: 9401748209

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During the past ten years, great advances have been made in the area of plant molecular biology. Such formerly esoteric techniques as gene transfer and plant regeneration are now routinely performed, making the dissection of regulatory elements of genes a common practice in many laboratories. Along with this new technology has come an almost bewildering array of rapidly changing techniques, often making it difficult for the novice to select and perform the technique most appropriate for answering a given biological question. In 1986, some of us felt that many of these techniques had become routine enough to warrant the publication of a laboratory manual. The manual is designed both for advanced college level laboratory courses and as a 'bench guide' for use in the scientific laboratory. Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised. Additional chapters that can replace or be added to this first edition will be published periodically. The editors recognize that many of the techniques described in this manual depend upon specialized plant genetic material, microbial strains, or recombinant plasmids. Those people desiring such material should contact the relevant authors directly. A list of the various contributors to this manual, including their addresses, is included.


Molecular Biology Techniques

Molecular Biology Techniques
Author: Heather B. Miller
Publisher: Academic Press
Total Pages: 227
Release: 2011-10-18
Genre: Science
ISBN: 0123855454

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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


Plant Molecular Biology

Plant Molecular Biology
Author: P. G. Jones
Publisher:
Total Pages: 248
Release: 1997-10-09
Genre: Nature
ISBN:

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The authors describe the most important currently used protocols in plant molecular biology. They give useful tips on stopping points and troubleshooting as well as providing information on safety. Lists of suppliers are included.


A Laboratory Guide for Cellular and Molecular Plant Biology

A Laboratory Guide for Cellular and Molecular Plant Biology
Author: I. Negrutiu
Publisher: Birkhäuser
Total Pages: 383
Release: 2013-03-09
Genre: Science
ISBN: 3034875029

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This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.


Plant Molecular Biology

Plant Molecular Biology
Author: USA (Ed. ). Gelvin, S. B., Purdue University, West Lafayette, IN
Publisher: Springer
Total Pages: 105
Release: 1993-01-01
Genre: Science
ISBN: 9780792322016

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A comprehensive manual dealing with the molecular aspects of plant molecular biology, which is designed both as a teaching aid for advanced laboratory courses and as a guide for laboratory research. Subjects covered include the introduction of genes into plants, assays of gene activity, plant nucleic acid extraction and characterization procedures, and the use of selectable and screenable markers in transgenic plants. Special emphasis is placed on the use of Agrobacterium tumefaciens to introduce genes into plants, but other gene introduction techniques are also presented. Each chapter, written by experts in the field, includes a discussion of the background to each technique, detailed protocols, media and solution formulas, and an extensive bibliography.


Human Molecular Biology Laboratory Manual

Human Molecular Biology Laboratory Manual
Author: Stefan Surzycki
Publisher: John Wiley & Sons
Total Pages: 246
Release: 2008-04-15
Genre: Science
ISBN: 0470999241

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Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use