Phospholipid Signaling Protocols PDF Download
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| Author | : Ian Bird |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 408 |
| Release | : 1998-04-15 |
| Genre | : Medical |
| ISBN | : |
Download Phospholipid Signaling Protocols Book in PDF, ePub and Kindle
This wide-ranging collection of state-of-the-art techniques for the assay of phospholipid and phospholipid-derived second messengers allows the identification and quantification of signal pathway activation. The assays described cover all the major phospholipases (C, D, A2), as well as sphingomyelinase and associated metabolites. Additional protocols are provided for the assay of phosphoinositide 3-, 4-, and 5-kinase activity and for the separation and identification of phospholipids, diacylglycerol and sphingolipids, as well as their phosphoinositol, choline, and fatty acid metabolites. Detailed, step-by-step instructions make this book suitable for both the newcomer and the experienced investigator.
| Author | : Teun Munnik |
| Publisher | : Springer Nature |
| Total Pages | : 298 |
| Release | : 2013-05-17 |
| Genre | : Science |
| ISBN | : 1627034013 |
Download Plant Lipid Signaling Protocols Book in PDF, ePub and Kindle
As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
| Author | : Mark Waugh |
| Publisher | : Humana Press |
| Total Pages | : 257 |
| Release | : 2016-08-23 |
| Genre | : Science |
| ISBN | : 9781493950072 |
Download Lipid Signaling Protocols Book in PDF, ePub and Kindle
In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
| Author | : Banafshé Larijani |
| Publisher | : Humana Press |
| Total Pages | : 0 |
| Release | : 2008-11-11 |
| Genre | : Science |
| ISBN | : 9781588297273 |
Download Lipid Signaling Protocols Book in PDF, ePub and Kindle
Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular BiologyTM series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls. Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology.
| Author | : John M. Walker |
| Publisher | : |
| Total Pages | : |
| Release | : 1984 |
| Genre | : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols |
| ISBN | : |
Download Methods in Molecular Biology: Phospholipid signaling protocols Book in PDF, ePub and Kindle
| Author | : Marco Brotto |
| Publisher | : Springer Nature |
| Total Pages | : 269 |
| Release | : |
| Genre | : |
| ISBN | : 1071639021 |
Download Lipid Signalling Book in PDF, ePub and Kindle
| Author | : Robert C. Dickson |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 331 |
| Release | : 2008-02-02 |
| Genre | : Science |
| ISBN | : 1592598161 |
Download Signal Transduction Protocols Book in PDF, ePub and Kindle
In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.
| Author | : Christopher J. Barker |
| Publisher | : Humana |
| Total Pages | : 260 |
| Release | : 2010-05-27 |
| Genre | : Science |
| ISBN | : |
Download Inositol Phosphates and Lipids Book in PDF, ePub and Kindle
This text introduces the basic methodological tools to measure inositol lipids and phosphates and also describes new approaches that have become available in the last ten years, including g RNA-silencing and the protocols involving inositol pyrophosphates.
| Author | : Jose M. Mato |
| Publisher | : CRC Press |
| Total Pages | : 225 |
| Release | : 2018-01-18 |
| Genre | : Medical |
| ISBN | : 1351092421 |
Download Phospholipid Metabolism in Cellular Signaling Book in PDF, ePub and Kindle
Phospholipids are no longer considered exclusively as the building blocks of biomembranes, but are now regarded to be exceptionally important to cellular signalling.Another conclusion resulting from studies over the past several years is that no single general scheme for the role of phospholipids during cell activation can be drawn as representative of all cells. This book presents a comprehensive view of the recent advances made in certain major research areas within the field of phospholipid metabolism in cellular signalling. Topics discussed within this volume include lipid composition of cellular membranes and their organization in biological systems, the dynamic aspects of phospholipid metabolism and its regulation by extracellular stimuli, and the role of newly discovered glycosyl-phosphatidylinositols in insulin action . The book also discusses two biologically active phospholipids: the sphingolipids, and ether-linked glycerophospholipids (the platelet activating factor). Phospholipid Metabolism in Cellular Signaling is an important research reference that should be considered required reading by all scientists and graduate students working on cell activation (e.g., growth factors, hormones, and oncogenes).
| Author | : David G. Lambert |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 350 |
| Release | : 2008-02-04 |
| Genre | : Science |
| ISBN | : 1592599494 |
Download Calcium Signaling Protocols Book in PDF, ePub and Kindle
In the first edition of Calcium Signaling Protocols I began by writing “The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for their help and patience. I am also very grateful to Professor John M. Walker, the series editor, for his continued help and advice over the course of this project.
