Phenotypic And Molecular Analysis Of The Maternal Effect Associated With Mutations In The Clk 1 Gene Of Caenorhabditis Elegans PDF Download
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Author | : Jason Burgess |
Publisher | : |
Total Pages | : 190 |
Release | : 2002 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Phenotypic and Molecular Analysis of the Maternal Effect Associated with Mutations in the Clk-1 Gene of Caenorhabditis Elegans Book in PDF, ePub and Kindle
"Mutations in the Caenorhabditis elegans maternal-effect gene clk-1 result in a highly pleiotropic phenotype, characterized by a general slow down in embryonic and larval development, as well as a slowing down of adult behaviors including defecation, pharyngeal pumping and swimming. First generation homozygous clk-1 mutants descended from a heterozygous mother are fully rescued for these mutant phenotypes. It has been shown that CLK-1 protein is a hydroxylase that acts in the conversion of demethoxyubiquinone (DMQ) to 5-hydroxyubiquinone, in the ubiquinone (Q) biosynthesis pathway. Consequently, clk-1 mutants accumulate the Q9 precursor, DMQ9 (the subscript refers to the length of the isoprenoid side chain). Here, I show that the profound maternal rescue observed in clk-1 maternally rescued animals is due to presence of the CLK-1 protein throughout larval development, in sufficient amounts to catalyze the production of Q9. clk-1 mutants have been shown to have a dietary requirement for Q8 due to their inability to synthesize Q9. I demonstrate that clk-1 maternally rescued animals have sufficient amounts of Q 9 to complete larval development and produce an almost full brood when raised on a Q8 deficient E. coli strain. I also show that prolonged arrest at the first larval stage, which is likely to result in degradation of any maternally contributed mRNA or protein, brings about a Clk mutant phenotype in maternally rescued animals. Finally, I reveal that the Clk mutant phenotype can be rescued at any larval stage by ectopic expression of CLK-1, suggesting that there is no developmental window for the rescue of clk-1 mutants by CLK-1. These results identify perdurance of maternally contributed product throughout development as the mechanism that accounts for the maternal effect observed in clk-1 mutants." --
Author | : Cathy Caron |
Publisher | : |
Total Pages | : 146 |
Release | : 1995 |
Genre | : |
ISBN | : |
Download Phenotypic and Molecular Analysis of Zyg-1 Book in PDF, ePub and Kindle
Author | : Paula Ruth Martin |
Publisher | : |
Total Pages | : 182 |
Release | : 1991 |
Genre | : |
ISBN | : |
Download Genetic and Phenotypic Analysis of Grandchildless Loci in Caenorhabditis Elegans Book in PDF, ePub and Kindle
Author | : Carol Marie Garvin |
Publisher | : |
Total Pages | : 222 |
Release | : 1996 |
Genre | : |
ISBN | : |
Download Genetic and Phenotypic Characterization of Maternal-effect Sterile Genes in Caenorhabditis Elegans Book in PDF, ePub and Kindle
Author | : Yan Meng |
Publisher | : |
Total Pages | : 194 |
Release | : 2000 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Genes that Affect Development and Biological Timing in Caenorhabditis Elegans Book in PDF, ePub and Kindle
"In an effort to find new genes involved in development and the biological timing, I carried out a new screen for viable maternal-effect mutations similar in protocol to the previous screen in which 24 such genes have been identified. I screened 10,600 genomes and isolated 6 slow-growing mutations and 5 behavioral and morphological mutations. Another maternal-effect slow-growing mutation is isolated from a screen for both maternal-effect and non maternal-effect slow development mutations. Genetic mapping suggests that none of the seven slow growing mutations corresponds to previously identified genes, so seven new clk genes (clk-4, clk-5, clk-6, clk-7 clk-8, clk-9, clk-10) have been identified. Because most identified clk genes (clk-2, clk-4 to clk-10 and gro-1) are defined by single allele, we believe that these genes have not yet been saturated. Mutants of seven new clk genes have typical Clk phenotypes: a mean lengthening of embryonic development, postembryonic development, defecation cycle and life span. As the screening procedure did not involve any measure of life span, it is suggested that slow life is sufficient for long life. As expected, all seven mutations can be maternally rescued." --
Author | : Paula Boutis |
Publisher | : |
Total Pages | : 114 |
Release | : 1995 |
Genre | : Animal mutation |
ISBN | : |
Download Viable Maternal-effect Mutations in the Nematode Caenorhabditis Elegans Book in PDF, ePub and Kindle
Author | : Niansheng N Cheng |
Publisher | : |
Total Pages | : 334 |
Release | : 1991 |
Genre | : |
ISBN | : |
Download Genetic and Developmental Analysis of Par-2, a Gene Required for Cytoplasmic Localization and Cleavage Patterning in Caenorhabditis Elegans Book in PDF, ePub and Kindle
Author | : Thi Phuong Anh Nguyen |
Publisher | : |
Total Pages | : 204 |
Release | : 2005 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Download Genetic and Phenotypic Analysis of Clk-1 Growth Suppressors in Caenorhabditis Elegans Book in PDF, ePub and Kindle
"Ubiquinone (UQ) is a lipid found in all cellular membranes. It is involved in multiple cellular processes, either directly or through its effect on the redox status of the cell. clk-1 encodes a highly conserved hydroxylase required for UQ biosynthesis. In C. elegans, mutations in clk-1 result in the accumulation of an UQ precursor, DMQ, and a pleiotropic phenotype in the mutants characterized by the slowing down of development, behaviors and aging. Additionally, in the absence of dietary UQ, clk-1 mutants also show a transient growth arrest and are sterile. Mutants that can suppress both sets of phenotypes in the point mutant clk-1(e2519) have been isolated. Their suppression patterns indicate that various aspects of the clk-1 phenotype can be uncoupled from each other. Furthermore, the analysis of their quinone content suggests that the phenotypes on UQ-producing bacteria are caused by the inability of dietary UQ to completely substitute for endogenous UQ. These suppressors carry mutations in tRNA genes, and thus to our knowledge, they are the first tRNA missense suppressors found in any metazoan." --
Author | : Cheryl Lynn Johnson |
Publisher | : |
Total Pages | : |
Release | : 2012 |
Genre | : |
ISBN | : 9780494971086 |
Download Regulation of Germ-line Expression of the Caenorhabditis Elegans Gene Fem-1 by Maternal Transcripts Book in PDF, ePub and Kindle
Author | : Ryuji Minasaki |
Publisher | : |
Total Pages | : 59 |
Release | : 2007 |
Genre | : |
ISBN | : |
Download Genetic and Molecular Characterization of Two Maternal Effect Genes Required for Caenorhabditis Elegans Development Book in PDF, ePub and Kindle