Peptide Characterization And Application Protocols PDF Download
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Author | : Gregg B. Fields |
Publisher | : Humana Press |
Total Pages | : 342 |
Release | : 2010-11-19 |
Genre | : Science |
ISBN | : 9781617376375 |
Download Peptide Characterization and Application Protocols Book in PDF, ePub and Kindle
This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.
Author | : Marie-Isabel Aguilar |
Publisher | : Springer Science & Business Media |
Total Pages | : 411 |
Release | : 2008-02-03 |
Genre | : Science |
ISBN | : 1592597424 |
Download HPLC of Peptides and Proteins Book in PDF, ePub and Kindle
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Author | : Vibin Ramakrishnan |
Publisher | : Springer Nature |
Total Pages | : 142 |
Release | : 2023-08-02 |
Genre | : Science |
ISBN | : 1071634054 |
Download Biophysical Characterization of Functional Peptides Book in PDF, ePub and Kindle
This protocol book presents reproducible and step-by-step procedures for the peptide synthesis, their characterization and applications. The volume includes an introductory section on in silico modelling of new peptide molecules, Molecular Dynamics Simulations, Docking, Electrostatic fingerprinting of peptides, and other modelling tools for peptide designing and optimization. Further, it covers protocols for the solid phase peptide synthesis, chromatographic and mass spectrometric characterization of peptides. Importantly, it covers methods for biophysical characterizations of peptides for their potential applications as drug delivery vehicles, peptide nano-assembly, bionanocatalysis, protein aggregation diseases, and peptide-based anti-bacterial.
Author | : Paul T. Matsudaira |
Publisher | : Elsevier |
Total Pages | : 205 |
Release | : 2012-12-02 |
Genre | : Science |
ISBN | : 0080924611 |
Download A Practical Guide to Protein and Peptide Purification for Microsequencing Book in PDF, ePub and Kindle
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing
Author | : William S. Hancock |
Publisher | : CRC Press |
Total Pages | : 280 |
Release | : 1995-10-23 |
Genre | : Medical |
ISBN | : 9780849378225 |
Download New Methods in Peptide Mapping for the Characterization of Proteins Book in PDF, ePub and Kindle
This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Author | : Ben M. Dunn |
Publisher | : Humana |
Total Pages | : 335 |
Release | : 2013-12-06 |
Genre | : Science |
ISBN | : 9781489940049 |
Download Peptide Analysis Protocols Book in PDF, ePub and Kindle
As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.
Author | : John M. Walker |
Publisher | : Humana |
Total Pages | : 512 |
Release | : 1994-04-26 |
Genre | : Medical |
ISBN | : |
Download Basic Protein and Peptide Protocols Book in PDF, ePub and Kindle
Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Author | : Gunnar Houen |
Publisher | : Springer Nature |
Total Pages | : 270 |
Release | : |
Genre | : |
ISBN | : 1071639145 |
Download Peptide Antibodies Book in PDF, ePub and Kindle
Author | : Catherine Cooper |
Publisher | : Springer Science & Business Media |
Total Pages | : 268 |
Release | : 2008-02-05 |
Genre | : Science |
ISBN | : 1592590470 |
Download Amino Acid Analysis Protocols Book in PDF, ePub and Kindle
A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.
Author | : Paul Cutler |
Publisher | : Springer Science & Business Media |
Total Pages | : 474 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 159259655X |
Download Protein Purification Protocols Book in PDF, ePub and Kindle
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.