Oxidative Folding Of Proteins PDF Download
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Author | : Luis Moroder |
Publisher | : Royal Society of Chemistry |
Total Pages | : 453 |
Release | : 2009 |
Genre | : Science |
ISBN | : 0854041486 |
Download Oxidative Folding of Peptides and Proteins Book in PDF, ePub and Kindle
With contributions from experts in the field, this book provides a comprehensive overview of the oxidative folding of cysteine-rich peptides.
Author | : Matthias J Feige |
Publisher | : Royal Society of Chemistry |
Total Pages | : 450 |
Release | : 2018-07-30 |
Genre | : Science |
ISBN | : 1782629904 |
Download Oxidative Folding of Proteins Book in PDF, ePub and Kindle
The formation of disulphide bonds is probably the most influential modification of proteins. These bonds are unique among post-translational modifications of proteins as they can covalently link cysteine residues far apart in the primary sequence of a protein. This has the potential to convey stability to otherwise marginally stable structures of proteins. However, the reactivity of cysteines comes at a price: the potential to form incorrect disulphide bonds, interfere with folding, or even cause aggregation. An elaborate set of cellular machinery exists to catalyze and guide this process: facilitating bond formation, inhibiting unwanted pairings and scrutinizing the outcomes. Only in recent years has it become clear how intimately connected this cellular machinery is with protein folding helpers, organellar redox balance and cellular homeostasis as a whole. This book comprehensively covers the basic principles of disulphide bond formation in proteins and describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins. The biotechnological and pharmaceutical relevance of proteins, their variants and synthetic replicates is continuously increasing. Consequently this book is an invaluable resource for protein chemists involved in realted research and production.
Author | : Rowen J. Y. Chang |
Publisher | : Springer Science & Business Media |
Total Pages | : 290 |
Release | : 2011-08-12 |
Genre | : Science |
ISBN | : 1441972730 |
Download Folding of Disulfide Proteins Book in PDF, ePub and Kindle
This book aims to cover the knowledge of protein folding accumulated from studies of disulfide-containing proteins, including methodologies, folding pathways, and folding mechanism of numerous extensively characterized disulfide proteins. Folding of Disulfide Proteins will be valuable supplementary reading for general biochemistry, biophysics, molecular biology, and cellular biology courses for graduate and undergraduate students. This book can also be used for specialized graduate-level biochemistry, biophysics, and molecular biology courses dedicated to protein folding as well as related biological problems and diseases. Will also be of interest to everybody interested in problems related to protein folding, and anyone who is interested in understanding the mechanism of protein misfolding and protein misfolding-related diseases.
Author | : Pumtiwitt C. Rancy |
Publisher | : |
Total Pages | : |
Release | : 2010 |
Genre | : Oxidases |
ISBN | : 9781124087627 |
Download Oxidative Protein Folding in Vitro Book in PDF, ePub and Kindle
Oxidative protein folding describes the process by which disulfide bonds are inserted into proteins as they fold into their native structure. This involves two distinct phases, an oxidation phase where these covalent linkages are first introduced, and an isomerization phase in which incorrectly placed disulfides are shuffled leading to the native pairings. In eukaryotes, disulfide bond formation can be catalyzed by a number of flavin-dependent sulfhydryl oxidases. This dissertation work investigates how a particular flavin-dependent sulfhydryl oxidase, Quiescin-sulfhydryl oxidase (QSOX), cooperates with protein disulfide isomerase (PDI) to generate native pairings in two unfolded reduced proteins: ribonuclease A (RNase A, four disulfide bonds and 105 disulfide isomers of the fully oxidized protein) and avian riboflavin binding protein (RfBP, nine disulfide bonds and more than 34 million corresponding disulfide pairings). This QSOX/PDI in vitro folding system involves no functional interaction between the two enzymatic components; QSOX inserts disulfide bonds into protein substrates while PDI isomerizes the misplaced pairs to the native ones. Rapid refolding does not require glutathione or glutathione-based redox buffers. Refolding of RfBP is followed continuously by monitoring spectral changes experienced by the ligand, riboflavin, upon binding to the apoprotein. Efficient refolding of this protein only occurs with a large molar excess of reduced PDI over the folding client protein. These conditions likely mirror the environment of the endoplasmic reticulum lumen where small concentrations of nascent proteins are exposed to nearly mM levels of PDI. Subsequent studies performed in the absence of QSOX or redox buffers, explore the effectiveness of mixtures of oxidized and reduced PDI in refolding RfBP. Here, the fastest refolding of RfBP occurs with excess reduced PDI and just enough oxidized PDI to generate nine disulfides in the protein. The implications of these in vitro experiments for understanding oxidative folding processes in vivo are discussed. Although unfolded proteins have been proven to be excellent substrates of QSOX, a recent proposal suggests that it can also function in the generation of inter-domain and inter-protein disulfide bridges, where the substrates are already substantially or completely folded. This suggestion has been tested using wild type and mutant Escherichia coli thioredoxin as a model substrate. These folded substrates are, by comparison, poorly oxidized by QSOX which is consistent with the expected stringent steric requirements for efficient thiol/disulfide exchange reactions.
Author | : Benjamin Peng-Chu Tu |
Publisher | : |
Total Pages | : 246 |
Release | : 2003 |
Genre | : |
ISBN | : |
Download Biochemical Basis of Oxidative Protein Folding in the Endoplasmic Reticulum Book in PDF, ePub and Kindle
Author | : Howard J. Leung |
Publisher | : |
Total Pages | : 56 |
Release | : 2005 |
Genre | : |
ISBN | : |
Download Impact of an Easily Reducible Disulfide Bond on the Oxidative Folding Rate of Multi-disulfide-containing Proteins Book in PDF, ePub and Kindle
Author | : Roger H. Pain |
Publisher | : |
Total Pages | : 296 |
Release | : 1994 |
Genre | : Science |
ISBN | : |
Download Mechanisms of Protein Folding Book in PDF, ePub and Kindle
The past five years have seen a major leap forward in our understanding of the way proteins fold into their three-dimensional, functional conformations. The rapidly expanding literature covers in vivo as well as in vitro studies and forms the basis for an important biotechnology industry. In this volume, a group of leading scientists review and assess the experimental evidence that underpins these advances and look for signs of a general picture of how proteins fold. Contributors show how such conformational changes are leading to new insights into membrane translocation, pore formation, and the clinically important aggregation phenomena. Students and researchers of biochemistry and molecular biology will find this book to be the ideal introduction to an exciting field.
Author | : Veronica Gonzalez |
Publisher | : |
Total Pages | : |
Release | : 2008 |
Genre | : Endoplasmic reticulum |
ISBN | : |
Download The Role of Protein Disulfide Isomerase (PDI) in Oxidative Folding Book in PDF, ePub and Kindle
Author | : Cláudio M. Gomes |
Publisher | : CRC Press |
Total Pages | : 302 |
Release | : 2016-04-19 |
Genre | : Medical |
ISBN | : 1439809658 |
Download Protein Folding and Metal Ions Book in PDF, ePub and Kindle
The role of metal ions in protein folding and structure is a critical topic to a range of scientists in numerous fields, particularly those working in structural biology and bioinorganic chemistry, those studying protein folding and disease, and those involved in the molecular and cellular aspects of metals in biological systems. Protein Folding an
Author | : Ruoyu Xiao |
Publisher | : |
Total Pages | : 44 |
Release | : 2005 |
Genre | : |
ISBN | : 9789171402387 |
Download Protein Disulfide Isomerase Book in PDF, ePub and Kindle