Next Generation Sequencing Of Human Antibody Repertoires For Exploring B Cell Landscape Antibody Discovery And Vaccine Development PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Next Generation Sequencing Of Human Antibody Repertoires For Exploring B Cell Landscape Antibody Discovery And Vaccine Development PDF full book. Access full book title Next Generation Sequencing Of Human Antibody Repertoires For Exploring B Cell Landscape Antibody Discovery And Vaccine Development.
Author | : Jacob Glanville |
Publisher | : Frontiers Media SA |
Total Pages | : 231 |
Release | : 2020-08-21 |
Genre | : |
ISBN | : 2889639517 |
Download Next-Generation Sequencing of Human Antibody Repertoires for Exploring B-cell Landscape, Antibody Discovery and Vaccine Development Book in PDF, ePub and Kindle
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Author | : Brandon DeKosky |
Publisher | : Springer |
Total Pages | : 105 |
Release | : 2017-05-29 |
Genre | : Medical |
ISBN | : 3319585185 |
Download Decoding the Antibody Repertoire Book in PDF, ePub and Kindle
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases. Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells. The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Author | : Brandon James DeKosky |
Publisher | : |
Total Pages | : 290 |
Release | : 2015 |
Genre | : |
ISBN | : |
Download Decoding the Antibody Repertoire Book in PDF, ePub and Kindle
Next-generation (high throughput) DNA sequencing of immunoglobulin variable region and T-cell receptor gene repertoires is providing critical information for understanding adaptive immune responses and for diagnostic and therapeutic applications1−4. However, existing immune repertoire sequencing technologies yield data on only one of the two chains of immune receptors and thus cannot provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes5−7. This work directly addressed these limitations by developing two new technologies for sequencing the complementary DNA (cDNA) of multiple mRNA transcripts from isolated single cells with very high throughput. In these methods, cells are sequestered into individual compartments and lysed in situ to capture single-cell mRNA onto magnetic beads, then the magnetic beads are used as template for RT-PCR reactions inside emulsion droplets that physically link cDNA of multiple transcripts for subsequent analysis by high-throughput DNA sequencing. Experimental throughput of over 2x106 cells in a single day with antibody heavy and light chain pairing accuracy greater than 97% was demonstrated with in vitro expanded human B cells. These new single-cell sequencing technologies were also applied for rapid discovery of new human antibodies and for analysis of the human immune response to vaccination. Finally we applied the techniques developed here to gain new insights regarding development of the antibody repertoire through high-throughput and high-resolution examination of naïve and memory B-cell compartments in healthy human donors.
Author | : |
Publisher | : Elsevier |
Total Pages | : 444 |
Release | : 2023-08-30 |
Genre | : Computers |
ISBN | : 0443192014 |
Download Resistance to Anti-CD20 Antibodies and Approaches for Their Reversal Book in PDF, ePub and Kindle
Resistance to Anti-CD20 Antibodies and Approaches for Their Reversal presents in-depth content written by international experts in the study of resistance to anti-CD20 antibodies and approaches for their reversal. Anti-CD20 antibodies are used to achieve B cell depletion and are developed to treat B cell proliferative disorders, including non-Hodgkin’s lymphoma and chronic lymphocytic leukemia. In the past two decades, anti-CD20 antibodies have revolutionized the treatment of all B cell malignancies, however, there are patients that fail to respond to initial therapy or relapse sooner. This book explores new and existing avenues surrounding Anti-CD20 antibodies. In recent years, several next-generation anti-CD20 therapies have been developed but predicting and reversing resistance is still a challenging task. These areas are being actively studied as they represent a potential to improve anti-CD20 therapies and are discussed thoroughly in the book. It is a valuable resource for researchers, students and member of the biomedical and medical fields who want to learn more about resistance to anti-CD20 antibodies and their reversal. • Presentation of current research, critical analyses, in-depth literature reviews, and the latest clinical reports on anti-CD20 antibody treatment • Discussion of recent developments of anti-CD20 antibodies in cancer and noncancer diseases treatment, possible resistance mechanisms and their reversals, as well as the exciting therapeutic opportunities offered by anti-CD20 antibodies in combination with chemotherapy or other treatment modalities • Utilization of a number of diagrams to visually illustrate complex content and plenty of tables to summarize important information
Author | : Tim F. Greten |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2018-08-22 |
Genre | : Medical |
ISBN | : 9783319879116 |
Download Immunotherapy of Hepatocellular Carcinoma Book in PDF, ePub and Kindle
In this book we provide insights into liver – cancer and immunology. Experts in the field provide an overview over fundamental immunological questions in liver cancer and tumorimmunology, which form the base for immune based approaches in HCC, which gain increasing interest in the community due to first promising results obtained in early clinical trials. Hepatocellular carcinoma (HCC) is the third most common cause of cancer related death in the United States. Treatment options are limited. Viral hepatitis is one of the major risk factors for HCC, which represents a typical “inflammation-induced” cancer. Immune-based treatment approaches have revolutionized oncology in recent years. Various treatment strategies have received FDA approval including dendritic cell vaccination, for prostate cancer as well as immune checkpoint inhibition targeting the CTLA4 or the PD1/PDL1 axis in melanoma, lung, and kidney cancer. Additionally, cell based therapies (adoptive T cell therapy, CAR T cells and TCR transduced T cells) have demonstrated significant efficacy in patients with B cell malignancies and melanoma. Immune checkpoint inhibitors in particular have generated enormous excitement across the entire field of oncology, providing a significant benefit to a minority of patients.
Author | : National Research Council |
Publisher | : National Academies Press |
Total Pages | : 74 |
Release | : 1999-05-06 |
Genre | : Medical |
ISBN | : 0309173051 |
Download Monoclonal Antibody Production Book in PDF, ePub and Kindle
The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Author | : Anthony R. Rees |
Publisher | : Oxford Medical Histories |
Total Pages | : 385 |
Release | : 2015 |
Genre | : Medical |
ISBN | : 0199646570 |
Download The Antibody Molecule Book in PDF, ePub and Kindle
'The Antibody Molecule' is a beautifully illustrated review of the remarkable developments within immunology from the discovery of the antibody molecule to its exploitation in medicine and the scientists and pioneers who were involved. This engaging and authoritative history will appeal to a wide audience
Author | : Marie-Paule Lefranc |
Publisher | : Academic Press |
Total Pages | : 472 |
Release | : 2001-05-23 |
Genre | : Medical |
ISBN | : 0080574475 |
Download The Immunoglobulin FactsBook Book in PDF, ePub and Kindle
The FactsBook series has established itself as the best source of easily accessible and accurate facts about protein groups. Books in the series use an easy-to-follow format and are meticulously researched and compiled by experts in the field.The Immunoglobulin FactsBook is the first published reference for all 203 human functional and ORF immunoglobulin genes. It is complete and standardized and employs nomenclature approved by the HUGO Nomenclature Committee.
Author | : Gabriele Neumann (Professor at the Influenza Research Institute) |
Publisher | : |
Total Pages | : |
Release | : 2020 |
Genre | : Influenza |
ISBN | : 9781621823087 |
Download Influenza Book in PDF, ePub and Kindle
"Influenza is a serious disease that affects millions worldwide every year. This book discusses cutting edge research on the viruses that cause the disease, its effects on the host, and current vaccine design strategies"--
Author | : Shailza Singh |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2021-04-25 |
Genre | : Medical |
ISBN | : 9789811533525 |
Download Systems and Synthetic Immunology Book in PDF, ePub and Kindle
Systems and Synthetic Immunology focuses on the similarities between biology and engineering at the systems level, which are important for applying engineering theories to biology problems. With the advent of new genomic techniques, there are numerous systematic investigations underway in the scientific world. This volume highlights techniques that can be used to effectively combine two of the most essential biological fields - Systems Biology and Synthetic Immunology. The respective chapters discuss the role of synthetic immunology in biotechnology, production of biomaterials, and their use in vaccine delivery. Further topics include the importance of cytokines; the use of genomic engineering tools in immunotherapy; immunosensors; nanotherapeutics; and bioinformatics tools in biomedical applications. Given its scope, the book offers readers an up-to-date and comprehensive review of this unique and dynamic field of research.