Natural Killer Cell Protocols PDF Download
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Author | : Kerry S. Campbell |
Publisher | : Springer Science & Business Media |
Total Pages | : 394 |
Release | : 2008-02-03 |
Genre | : Medical |
ISBN | : 1592590446 |
Download Natural Killer Cell Protocols Book in PDF, ePub and Kindle
In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.
Author | : Srinivas S. Somanchi |
Publisher | : Methods in Molecular Biology |
Total Pages | : 384 |
Release | : 2018-05-27 |
Genre | : Medical |
ISBN | : 9781493981168 |
Download Natural Killer Cells: Methods and Protocols Book in PDF, ePub and Kindle
Author | : Noriko Shimasaki |
Publisher | : Humana |
Total Pages | : 335 |
Release | : 2022-04-15 |
Genre | : Medical |
ISBN | : 9781071621592 |
Download Natural Killer (NK) Cells Book in PDF, ePub and Kindle
This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.
Author | : John M. Walker |
Publisher | : |
Total Pages | : |
Release | : 1984 |
Genre | : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols |
ISBN | : |
Download Methods in Molecular Biology: Natural killer cell protocols Book in PDF, ePub and Kindle
Author | : Michael T. Lotze |
Publisher | : Academic Press |
Total Pages | : 709 |
Release | : 2009-11-12 |
Genre | : Medical |
ISBN | : 0080919294 |
Download Natural Killer Cells Book in PDF, ePub and Kindle
Natural Killer Cells explains the importance of killer cells and how they are produced. It mentions that the most likely explanation for killer cell production is that they serve as a complementary system for T cells as a primary defense against viruses. However, these cells defend against certain viruses only, such as herpes viruses and influenza viruses. The book also explains the primary functions of killer cells, and it discusses how these cells help recognize damaged tissues, limit further damage to tissues, and regenerate damaged tissues. It discusses how these cells mature and develop, and it covers the different isolation, culture, and propagation methods of these cells. Furthermore, it focuses on the different killer cells that are present in various parts of the human body. The book concludes by explaining that natural killer cells are utilized for clinical therapy of malignancies, and that they have led to positive outcomes in the field of biology and medicine. Provides a broad, detailed coverage of the biology and interactions of NK cells for students, fellows, scientists, and practitioners Includes figures, histologic sections, and illustrations of the ontogeny of NK cells
Author | : Shoba Amarnath |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2020-03-09 |
Genre | : Medical |
ISBN | : 9781071603376 |
Download Innate Lymphoid Cells Book in PDF, ePub and Kindle
This volume explores the latest techniques used to characterize murine and human Innate Lymphoid Cells (ILC) subsets. This book also aims to discuss useful phenotypical and functional assays that may be relevant for studying ILC function in mice and humans. Chapters cover topics such as methods to study microbiome and enteroid cultures; characterizing ILC precursors; and cellular engineering of ILCs for therapeutic use. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Innate Lymphoid Cells: Methods and Protocols is a valuable research tool for anyone interested in ILC biology, and those interested in fundamental immunology, cellular engineering, cell signaling, and clinician scientists.
Author | : Joanna Picot |
Publisher | : Springer Science & Business Media |
Total Pages | : 357 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 1592598617 |
Download Human Cell Culture Protocols Book in PDF, ePub and Kindle
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author | : Paulette Mehta |
Publisher | : Jones & Bartlett Learning |
Total Pages | : 516 |
Release | : 2004 |
Genre | : Bone marrow |
ISBN | : 9780763718558 |
Download Pediatric Stem Cell Transplantation Book in PDF, ePub and Kindle
This textbook examines stem cell transplatation in pediatric patients.
Author | : Benjamin Bonavida |
Publisher | : Academic Press |
Total Pages | : 540 |
Release | : 2021-06-23 |
Genre | : Science |
ISBN | : 0128243961 |
Download Successes and Challenges of NK Immunotherapy Book in PDF, ePub and Kindle
Successes and Challenges of NK Immunotherapy: Increasing Anti-tumor Efficacy describes the unique therapeutic applications of NK cells to fight cancers and eliminate the bulk and subset of cancer stem cells responsible for metastasis, relapse and recurrences. The book provides information on the development, engineering, mechanisms of action, response to various preclinical models, and applications in various clinical trials. Sections cover the development of highly engineered cytotoxic NK cells, their mechanisms of action, preclinical and clinical applications, the development and application of CAR-NK cells, and new NK-drug conjugates, also emphasizing that activated NK cells can target and kill highly resistant cancer stem cells. Written by the leading experts on NK immunotherapy worldwide, this is a valuable resource for researchers, clinicians and members of the biomedical field who are interested in understanding novel and efficient therapies to fight cancers. Discusses the unique developmental applications of NK immunotherapy against cancers, which differs greatly from other types of immunotherapies Provides up-to-date and highly relevant information through chapters written by the leading researchers in the field Presents a significant number of schematic diagrams for easy understanding and reproducibility
Author | : Chaohong Liu |
Publisher | : Humana |
Total Pages | : 195 |
Release | : 2021-10-16 |
Genre | : Medical |
ISBN | : 9781071617748 |
Download Invariant Natural Killer T-Cells Book in PDF, ePub and Kindle
This detailed book focuses on various aspects of Invariant Natural Killer T-Cells (iNKT), which are known to contribute to homeostasis and autoimmunity and can also cause various pathological responses such as allergy, infection, excessive autoimmune response, and cancer. The volume explores techniques for identification and isolation of iNKT cells, iNKT cell activation and transformation, as well as proliferation and differentiation and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Invariant Natural Killer T-Cells: Methods and Protocols is an ideal guide for researchers working with and studying iNKT cells, perhaps to provide a deeper knowledge of the human immune system.