Nanosecond Fluorescence Spectroscopy of Membrane Probes
Author | : Edmund Dennis Matayoshi |
Publisher | : |
Total Pages | : |
Release | : 1979 |
Genre | : |
ISBN | : |
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Author | : Edmund Dennis Matayoshi |
Publisher | : |
Total Pages | : |
Release | : 1979 |
Genre | : |
ISBN | : |
Author | : Ruud Kraayenhof |
Publisher | : Springer Science & Business Media |
Total Pages | : 401 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 3642560679 |
The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
Author | : Leslie M. Loew |
Publisher | : CRC Press |
Total Pages | : 227 |
Release | : 2018-04-19 |
Genre | : Science |
ISBN | : 1351085255 |
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.
Author | : Christopher Randolph Rhodes |
Publisher | : |
Total Pages | : 106 |
Release | : 2012 |
Genre | : |
ISBN | : |
Cell membranes organize and regulate a vital share of biological signaling. Fluorescence has become the main analytical tool with which to study such systems, and novel fluorescence techniques drive biological discovery at ever-smaller size and time scales. By exploiting the unique photophysics of fluorescent probes, I have advanced two parallel approaches to studying membrane systems: nanoengineered biointerfaces and time-resolved spectroscopy. Metallic nanostructures were engineered to precisely localize fluorescence emission, enhance label-free detection of lipid-protein interactions, and manipulate the diffusion landscapes of supported lipid bilayers. A platform for fluorescence spectroscopy at sub-nanosecond resolution combines fluorescence cross-correlation spectroscopy (FCCS) and time-resolved fluorescence anisotropy (TRFA) to characterize protein diffusion. These techniques have provided insight into the organization and structural flexibility of intercellular adhesion molecule-1 (ICAM-1), oligomerization of the small GTPase H-Ras, and the association of diverse lipid anchor domains in vitro and in vivo. Finally, I demonstrate progress toward single-molecule characterization of lipid-protein systems, and elaborate on its relevance to environmental biosensors for public health and biosecurity applications.
Author | : Godfrey S. Beddard |
Publisher | : |
Total Pages | : 256 |
Release | : 1981 |
Genre | : Science |
ISBN | : |
The photophysical and photochemical properties of probes in biological and in model systems are topics of intense current research. The wavelength, intensity, time and polarisation dependence of fluorescence probes can provide information on properties as diverse as electric fields and changes in conformation and may be used in the formidable task of establishing structure-function relationships in proteins and cell membranes. The wide scope of interactions which can be studied by these fluorescent molecules is illustrated in the articles in this volume which are based on talks, although much extended and updated, given at a meeting on 'Fluorescence Probes in Proteins and Membranes' held in the Royal Institution in November 1979.
Author | : Leslie M. Loew |
Publisher | : Springer |
Total Pages | : 248 |
Release | : 1988-05-31 |
Genre | : Medical |
ISBN | : |
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.
Author | : Herwig J. Hilderson |
Publisher | : Springer Science & Business Media |
Total Pages | : 480 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 1461393590 |
As stated by its first editor, Dr. D. B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of different techniques and disciplines. This volume deals with the applications of fluorescence spectroscopy to membrane research. It seeks to present complementary biochemical and bio physical data on both the structure and the dynamics of biological membranes. Biophysics and biochemistry are improving more and more in their ability to study biomembranes, overlapping somewhat in this area and explaining the functioning of the whole cell in terms of the properties of its individual com ponents. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects. The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. Subsequent chapters dis cuss more specific applications in cells, biological membranes, and their con stituents (lipids, proteins).
Author | : Martin Hof |
Publisher | : Springer Science & Business Media |
Total Pages | : 315 |
Release | : 2006-01-27 |
Genre | : Science |
ISBN | : 3540270043 |
Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.
Author | : Leslie M. Loew |
Publisher | : CRC Press |
Total Pages | : 235 |
Release | : 2018-04-17 |
Genre | : Medical |
ISBN | : 135109369X |
The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls.
Author | : R. Cundall |
Publisher | : Springer Science & Business Media |
Total Pages | : 767 |
Release | : 2013-11-11 |
Genre | : Science |
ISBN | : 1475716346 |
At the time that the editors conceived the idea of trying to organize the meeting on which the contents of this volume are based and which became, in March 1980, a NATO Advanced Study Institute, the techniques of time-resolved fluorescence spectroscopy, in both the nanosecond and sub-nanosecond time-domains, might reasonably have been said to be coming of age, both in their execution and in the analysis and interpretation of the results obtained. These techniques, then as now, comprised mainly a number of pulse methods using laser, flash-lamp or, most recently, synchrotron radiation. In addition, significant developments in the more classical phase approach had also rendered that method popular, utilizing either modulation of an otherwise continuous source or, again recently, the ultra-rapid pulse rate attainable with a synchrotron source. In general terms, time-resolved fluorescence studies are capable, under appropriate conditions, of supplying direct kinetic information on both photophysics and various aspects of molecular, macromolecular and supramolecular structure and dynamics. The nanosecond and sub-nanosecond time-scales directly probed render these techniques particularly appropriate in studying relaxation and fluctuation processes in macromolecules, particularly biopolymers (e. g. proteins, nucleic acids), in supramolecular assemblies such as cell membranes, and in a variety of relatively simpler model systems.