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| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 321 |
| Release | : 1993-11-17 |
| Genre | : Science |
| ISBN | : 0080859372 |
Motility Assays for Motor Proteins
| Author | : Christopher P. Toseland |
| Publisher | : Springer |
| Total Pages | : 306 |
| Release | : 2014-08-05 |
| Genre | : Science |
| ISBN | : 3034808569 |
This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
| Author | : Fady Malik |
| Publisher | : |
| Total Pages | : 278 |
| Release | : 1993 |
| Genre | : Cells |
| ISBN | : |
| Author | : Miriam W. Allersma |
| Publisher | : |
| Total Pages | : 290 |
| Release | : 2000 |
| Genre | : |
| ISBN | : |
| Author | : Sushil K. Sarna |
| Publisher | : Biota Publishing |
| Total Pages | : 159 |
| Release | : 2010-11-01 |
| Genre | : Medical |
| ISBN | : 1615041516 |
Three distinct types of contractions perform colonic motility functions. Rhythmic phasic contractions (RPCs) cause slow net distal propulsion with extensive mixing/turning over. Infrequently occurring giant migrating contractions (GMCs) produce mass movements. Tonic contractions aid RPCs in their motor function. The spatiotemporal patterns of these contractions differ markedly. The amplitude and distance of propagation of a GMC are several-fold larger than those of an RPC. The enteric neurons and smooth muscle cells are the core regulators of all three types of contractions. The regulation of contractions by these mechanisms is modifiable by extrinsic factors: CNS, autonomic neurons, hormones, inflammatory mediators, and stress mediators. Only the GMCs produce descending inhibition, which accommodates the large bolus being propelled without increasing muscle tone. The strong compression of the colon wall generates afferent signals that are below nociceptive threshold in healthy subjects. However, these signals become nociceptive; if the amplitudes of GMCs increase, afferent nerves become hypersensitive, or descending inhibition is impaired. The GMCs also provide the force for rapid propulsion of feces and descending inhibition to relax the internal anal sphincter during defecation. The dysregulation of GMCs is a major factor in colonic motility disorders: irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), and diverticular disease (DD). Frequent mass movements by GMCs cause diarrhea in diarrhea predominant IBS, IBD, and DD, while a decrease in the frequency of GMCs causes constipation. The GMCs generate the afferent signals for intermittent short-lived episodes of abdominal cramping in these disorders. Epigenetic dysregulation due to adverse events in early life is one of the major factors in generating the symptoms of IBS in adulthood.
| Author | : Nicolas Giuseppone |
| Publisher | : John Wiley & Sons |
| Total Pages | : 448 |
| Release | : 2021-03-30 |
| Genre | : Science |
| ISBN | : 3527821988 |
A must-have resource that covers everything from out-of-equilibrium chemical systems and materials to dissipative self-assemblies Out-of-Equilibrium Supramolecular Systems and Materials presents a comprehensive overview of the synthetic approaches that use supramolecular bonds in various out-of-thermodynamic equilibrium situations. With contributions from noted experts on the topic, the text contains information on the design of dissipative self-assemblies that maintain their structures when fueled by an external source of energy. The contributors also examine molecules and nanoscale objects and materials that can produce mechanical work based on molecular machines. Additionally, the book explores non-equilibrium supramolecular polymers that can be trapped in kinetically stable states, as well as out-of-equilibrium chemical systems and oscillators that are important to understand the emergence of complex behaviors and, in particular, the origin of life. This important book: Offers comprehensive coverage of fields from design of dissipative self-assemblies to non-equilibrium supramolecular polymers Presents information on a highly emerging and interdisciplinary topic Includes contributions from internationally renowned scientists Written for chemists, physical chemists, biochemists, material scientists, Out-of-Equilibrium Supramolecular Systems and Materials is an indispensable resource written by top scientists in the field.
| Author | : Ken Dill |
| Publisher | : Garland Science |
| Total Pages | : 845 |
| Release | : 2010-10-21 |
| Genre | : Science |
| ISBN | : 1136672982 |
Molecular Driving Forces, Second Edition E-book is an introductory statistical thermodynamics text that describes the principles and forces that drive chemical and biological processes. It demonstrates how the complex behaviors of molecules can result from a few simple physical processes, and how simple models provide surprisingly accurate insights into the workings of the molecular world. Widely adopted in its First Edition, Molecular Driving Forces is regarded by teachers and students as an accessible textbook that illuminates underlying principles and concepts. The Second Edition includes two brand new chapters: (1) "Microscopic Dynamics" introduces single molecule experiments; and (2) "Molecular Machines" considers how nanoscale machines and engines work. "The Logic of Thermodynamics" has been expanded to its own chapter and now covers heat, work, processes, pathways, and cycles. New practical applications, examples, and end-of-chapter questions are integrated throughout the revised and updated text, exploring topics in biology, environmental and energy science, and nanotechnology. Written in a clear and reader-friendly style, the book provides an excellent introduction to the subject for novices while remaining a valuable resource for experts.
| Author | : Stanley A. Cohn |
| Publisher | : John Wiley & Sons |
| Total Pages | : 483 |
| Release | : 2021-09-08 |
| Genre | : Science |
| ISBN | : 1119526353 |
DIATOM GLIDING MOTILITY Moving photosynthetic organisms are still a great mystery for biologists and this book summarizes what is known and reports the current understanding and modeling of those complex processes. The book covers a broad range of work describing our current state of understanding on the topic, including: historic knowledge and misconceptions of motility; evolution of diatom motility; diatom ecology & physiology; cell biology and biochemistry of diatom motility, anatomy of motile diatoms; observations of diatom motile behavior; diatom competitive ability, unique forms of diatom motility as found in the genus Eunotia; and models of motility. This is the first book attempting to gather such information surrounding diatom motility into one volume focusing on this single topic. Readers will be able to gather both the current state of understanding on the potential mechanisms and ecological regulators of motility, as well as possible models and approaches used to help determine how diatoms accomplish such varied behaviors as diurnal movements, accumulation into areas of light, niche partitioning to increase species success. Given the fact that diatoms remain one of the most ecologically crucial cells in aquatic ecosystems, we hope that this volume will act as a springboard towards future research into diatom motility and even better resolution of some of the issues in motility. Audience Diatomists, phycologists, aquatic ecologists, cellular physiologists, environmental biologists, biophysicists, diatom nanotechnologists, algal ecologists, taxonomists.
| Author | : Bruce Alberts |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cytology |
| ISBN | : 9780815332183 |
| Author | : Adam Z. Hartman |
| Publisher | : |
| Total Pages | : 308 |
| Release | : 2008 |
| Genre | : Microtubules |
| ISBN | : 9780549675556 |
Molecular motors and microtubules have for a long time been objects of great interest in biophysics. Both represent elegant examples of nature's ability to convert the energy of a chemical reaction to the production of positive mechanical work. But the ability to utilize this energy in a consistent, macroscopically evident manner is complicated by the difficulty inherent in motor protein patterning and random stepping. In this work, a new architecture utilizing the motor protein kinesin, and nanocapillaries grown in an AAO membrane is demonstrated which takes advantage of cylindrical geometric patterning. Ensembles of kinesins in these nanocapillaries are shown to exert forces several times that of a single motor protein, by means of a microtubule force-velocity motility assay modified to the AAO membrane. Further, the kinesin ensembles are shown to exhibit enhanced behavior in the presence of an appropriately applied AC electric field. Results are discussed in the context of modified theories, and potential applications to the fields of molecular transport and selective separation are also discussed.
