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Molecular Methods for Virus Detection

Molecular Methods for Virus Detection
Author: Danny L. Wiedbrauk
Publisher: Elsevier
Total Pages: 405
Release: 1995-02-08
Genre: Science
ISBN: 0080536891

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Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables


Molecular Virology of Human Pathogenic Viruses

Molecular Virology of Human Pathogenic Viruses
Author: Wang-Shick Ryu
Publisher: Academic Press
Total Pages: 441
Release: 2016-03-30
Genre: Science
ISBN: 0128009993

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Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author’s more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. Presents viruses within their family structure Contains recommended journal articles with perspectives to put primary literature in context Includes integrated recommended reading references within each chapter Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor’s manual, study guide, and test bank


Molecular Virology

Molecular Virology
Author: Susanne Modrow
Publisher: Springer
Total Pages: 0
Release: 2013-09-18
Genre: Science
ISBN: 9783642207174

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The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.


Global Infectious Disease Surveillance and Detection

Global Infectious Disease Surveillance and Detection
Author: Institute of Medicine
Publisher: National Academies Press
Total Pages: 284
Release: 2007-11-11
Genre: Medical
ISBN: 0309111145

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Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.


Molecular Methods in Plant Disease Diagnostics

Molecular Methods in Plant Disease Diagnostics
Author: Neil Boonham
Publisher: CABI
Total Pages: 214
Release: 2016-04-19
Genre: Science
ISBN: 1780641478

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Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.


Developing Norms for the Provision of Biological Laboratories in Low-Resource Contexts

Developing Norms for the Provision of Biological Laboratories in Low-Resource Contexts
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
Total Pages: 121
Release: 2019-05-01
Genre: Science
ISBN: 0309486564

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On June 27-28, 2018, the U.S. National Academies of Sciences, Engineering, and Medicine (the National Academies) convened an international workshop in Amsterdam, the Netherlands, on developing norms for the provision of laboratories in low-resource contexts. The U.S. Department of State's Biosecurity Engagement Program requested that the National Academies organize this workshop to engage an international group of organizations that provide funding for construction, upgrades, and maintenance of biological laboratories in countries without the means to build such labs themselves. Twenty-one people from 19 organizations participated. The intent was to advance the conversation about the identification and application of guiding principles and common norms for use by these organizations in their grants, partnerships, and aid. This publication summarizes the presentations and discussions from the workshop.


Basic and Applied Aspects of Biotechnology

Basic and Applied Aspects of Biotechnology
Author: Varsha Gupta
Publisher: Springer
Total Pages: 527
Release: 2016-10-22
Genre: Technology & Engineering
ISBN: 9811008752

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This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.


Manual of Commercial Methods in Clinical Microbiology

Manual of Commercial Methods in Clinical Microbiology
Author: Allan L. Truant
Publisher: American Society for Microbiology Press
Total Pages: 1557
Release: 2001-12-18
Genre: Medical
ISBN: 1555811892

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A general resource for all subdisciplines of clinical microbiology to use when evaluating commercial methods, tests, or procedures. • Reviews all the commercially available tests (both manual and automated) in the discipline of clinical microbiology. • Includes a description of the sensitivities, specificities, and predictive values from peer-reviewed sources. • Features separate chapters devoted to molecular microbiology, information management, emerging infectious diseases, and veterinary clinical microbiology.


Diagnostic Virology Protocols

Diagnostic Virology Protocols
Author: John R. Stephenson
Publisher: Springer Science & Business Media
Total Pages: 388
Release: 1998-08-28
Genre: Medical
ISBN: 9780896034792

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A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.


Molecular Detection of Human Viral Pathogens

Molecular Detection of Human Viral Pathogens
Author: Dongyou Liu
Publisher: CRC Press
Total Pages: 1152
Release: 2016-04-19
Genre: Medical
ISBN: 1439812373

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Despite being recognized and fought against over countless centuries, human viral pathogens continue to cause major public health problems worldwide-killing millions of people and costing billions of dollars in medical care and lost productivity each year. With contributions from specialists in their respective areas of viral pathogen research, Mol