Molecular Biology And Genetic Engineering Of Yeasts PDF Download
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Author | : Henri Heslot |
Publisher | : CRC Press |
Total Pages | : 818 |
Release | : 2018-01-18 |
Genre | : Technology & Engineering |
ISBN | : 1351091638 |
Download Molecular Biology and Genetic Engineering of Yeasts Book in PDF, ePub and Kindle
Molecular Biology and Genetic Engineering of Yeasts presents a comprehensive examination of how yeasts are used in genetic engineering. The book discusses baker's yeast, in addition to a number of unconventional yeasts being used in an increasing number of studies. 175 figures help illustrate the information presented. Topics discussed include yeast transformation, yeast plasmids, protein localization and processing in yeast, protein secretion, various aspects of Saccharomyces cerevisiae, and heterologous expression and secretion.
Author | : J.F.T. Spencer |
Publisher | : Springer Science & Business Media |
Total Pages | : 546 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 1461254914 |
Download Yeast Genetics Book in PDF, ePub and Kindle
During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. There great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspectiv(}-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily.
Author | : Valeria Mapelli |
Publisher | : Springer Nature |
Total Pages | : 300 |
Release | : 2022-07-04 |
Genre | : Science |
ISBN | : 1071623990 |
Download Yeast Metabolic Engineering Book in PDF, ePub and Kindle
This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Author | : |
Publisher | : Gulf Professional Publishing |
Total Pages | : 961 |
Release | : 2004-05-14 |
Genre | : Science |
ISBN | : 0080575137 |
Download Guide to Yeast Genetics and Molecular Biology Book in PDF, ePub and Kindle
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein
Author | : Jeffrey S. Smith |
Publisher | : Humana |
Total Pages | : 0 |
Release | : 2016-09-17 |
Genre | : Science |
ISBN | : 9781493951826 |
Download Yeast Genetics Book in PDF, ePub and Kindle
Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
Author | : Alan Wiseman |
Publisher | : Prentice Hall |
Total Pages | : 216 |
Release | : 1991 |
Genre | : Science |
ISBN | : |
Download Genetically-engineered Proteins and Enzymes from Yeasts Book in PDF, ePub and Kindle
Presents the important concepts and advances on how to successfully use genetic engineering techniques to obtain human proteins and enzymes - an area of vital importance for the production of human proteins for medical purposes.
Author | : Gordon E. Russell |
Publisher | : Intercept Limited |
Total Pages | : 184 |
Release | : 1988-01 |
Genre | : Science |
ISBN | : 9780946707157 |
Download Yeast Biotechnology Book in PDF, ePub and Kindle
This collection of review articles covering the molecular biology and biotechnology of yeasts is compiled from the interdisciplinary series of books entitled Biotechnology and Genetic Engineering Reviews.
Author | : John R. Johnston |
Publisher | : Oxford University Press, USA |
Total Pages | : 316 |
Release | : 1994 |
Genre | : Medical |
ISBN | : |
Download Molecular Genetics of Yeast Book in PDF, ePub and Kindle
The book covers comprehensively all current experimental procedures used in the research of the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Featuring detailed protocols and practical tips, it guarantees easy access to a wide range of specialized topics within thisrapidly advancing field. Internationally-recognized experts present all methods currently in use, discussing topics such as DNA isolulation, cloning and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and mutant isolulation. Chapters on Ty insertionalmutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast are also included. Researchers in the fields of molecular biology, genetics, and biochemistry working with this yeast, as well as professionals of thebiotechnology industry will refer to this practical reference frequently.
Author | : Johannes H. de Winde |
Publisher | : Springer Science & Business Media |
Total Pages | : 375 |
Release | : 2008-01-26 |
Genre | : Science |
ISBN | : 354037003X |
Download Functional Genetics of Industrial Yeasts Book in PDF, ePub and Kindle
In recent years, new yeast species have proven their value and novel biotechnological applications have emerged. This book compiles the multi-faceted genetic repertoire of several yeasts relevant to modern biotechnology, and describes their utilization in research and application in the light of their genetic make-up and physiological characteristics. Moreover, the book presents a thorough overview of a wide array of methodologies from classical genetics to modern genomics technologies that have been and are being used in functional analysis of yeasts.
Author | : Horst Feldmann |
Publisher | : John Wiley & Sons |
Total Pages | : 1 |
Release | : 2012-09-06 |
Genre | : Science |
ISBN | : 3527659196 |
Download Yeast Book in PDF, ePub and Kindle
Finally, a stand-alone, all-inclusive textbook on yeast biology. Based on the feedback resulting from his highly successful monograph, Horst Feldmann has totally rewritten he contents to produce a comprehensive, student-friendly textbook on the topic. The scope has been widened, with almost double the content so as to include all aspects of yeast biology, from genetics via cell biology right up to biotechnology applications. The cell and molecular biology sections have been vastly expanded, while information on other yeast species has been added, with contributions from additional authors. Naturally, the illustrations are in full color throughout, and the book is backed by a complimentary website. The resulting textbook caters to the needs of an increasing number of students in biomedical research, cell and molecular biology, microbiology and biotechnology who end up using yeast as an important tool or model organism.