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Microscopy, Immunohistochemistry, and Antigen Retrieval Methods

Microscopy, Immunohistochemistry, and Antigen Retrieval Methods
Author: M.A. Hayat
Publisher: Springer Science & Business Media
Total Pages: 368
Release: 2007-05-08
Genre: Medical
ISBN: 0306475995

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Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.


Antigen Retrieval Techniques

Antigen Retrieval Techniques
Author: Shan-Rong Shi
Publisher: Eaton Publishing Company/Biotechniques Books
Total Pages: 388
Release: 2000
Genre: Medical
ISBN:

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Immunohistochemistry: Basics and Methods

Immunohistochemistry: Basics and Methods
Author: Igor B. Buchwalow
Publisher: Springer Science & Business Media
Total Pages: 153
Release: 2010-01-26
Genre: Medical
ISBN: 3642046096

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This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.


Biobanking

Biobanking
Author: William H. Yong
Publisher: Humana Press
Total Pages: 470
Release: 2018-12-14
Genre: Medical
ISBN: 9781493989331

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This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.


Antigen Retrieval Immunohistochemistry Based Research and Diagnostics

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics
Author: Shan-Rong Shi
Publisher: John Wiley & Sons
Total Pages: 367
Release: 2011-01-14
Genre: Science
ISBN: 111806030X

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The most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects. Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work: Summarizes the key problems in the four fields of antigen retrieval Discusses the advances of AR techniques and their applications Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC Includes an appendix of related laboratory protocols Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products. Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor


Immunohistochemistry in Diagnostic Dermatopathology

Immunohistochemistry in Diagnostic Dermatopathology
Author: Mai P. Hoang
Publisher: Cambridge University Press
Total Pages: 315
Release: 2017-09-28
Genre: Medical
ISBN: 1107150167

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One of only a few textbooks on the market dedicated to the important role of immunohistochemistry in diagnostic dermatopathology.


Immunohistochemistry and Immunocytochemistry

Immunohistochemistry and Immunocytochemistry
Author: Simon Renshaw
Publisher: John Wiley & Sons
Total Pages: 258
Release: 2017-02-06
Genre: Science
ISBN: 1118717775

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Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.


Resin Microscopy and On-Section Immunocytochemistry

Resin Microscopy and On-Section Immunocytochemistry
Author: Geoffrey R. Newman
Publisher: Springer Science & Business Media
Total Pages: 291
Release: 2012-12-06
Genre: Science
ISBN: 3642569307

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Since antibodies tagged with markers have been developed, immunocytochemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.


Morphology Methods

Morphology Methods
Author: Ricardo V. Lloyd
Publisher: Springer Science & Business Media
Total Pages: 435
Release: 2001-06-15
Genre: Medical
ISBN: 1592591906

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The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.