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Protocols for Micropropagation of Woody Trees and Fruits

Protocols for Micropropagation of Woody Trees and Fruits
Author: S.Mohan Jain
Publisher: Springer Science & Business Media
Total Pages: 548
Release: 2007-09-18
Genre: Technology & Engineering
ISBN: 1402063520

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Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.


Micropropagation of Woody Trees and Fruits

Micropropagation of Woody Trees and Fruits
Author: S.M. Jain
Publisher: Springer Science & Business Media
Total Pages: 834
Release: 2012-12-06
Genre: Science
ISBN: 9401001251

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This book provides comprehensive information on micropropagation of economically important forest and fruit trees, which is usually available in scattered literature. Topics cover a wide range, from tropical forest and fruit trees for paper or food supply, to Prunus species for local craft bark production.


Micropropagation of Woody Plants

Micropropagation of Woody Plants
Author: M.R. Ahuja
Publisher: Springer Science & Business Media
Total Pages: 510
Release: 2013-06-29
Genre: Science
ISBN: 9401581169

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This volume covers recent advances in the vegetative propagation of woody plants by tissue culture. A wide range of topics relevant to micropropagation of woody plants are discussed by renowned international scientists. These include cellular contro of morphogenesis, light regimes in tissue culture, maturation and rejuvenation, synthetic seed, genetics of micropropagated plants, haploid embryogenesis, protoplast culture, and acclimatization of ex vitro woody plants. In addition to micropropagation of selected woody plants, both gymnosperms and angiosperms, this volume also includes in vitro genetic selection, strategic planning for application of biotechnology for genetics and breeding, and clonal options for woody plant improvement. A balanced view of both perspectives and limitations of woody plant micropropagation is presented.


Tissue Culture of Trees

Tissue Culture of Trees
Author: John H. Dodds
Publisher: Springer Science & Business Media
Total Pages: 157
Release: 2012-12-06
Genre: Science
ISBN: 1468466917

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1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually described as an un organised proliferating mass of tissue. Although callus cultures have a great deal of potential in the biotechnological aspects of tissue culture, i.e. secondary product formation, they are not very suitable for plant propagation. The key reason for their unsuitability is that genetic aber rations occur during mitotic divisions in callus growth (D'amato.l965). The aberrations can be of a major type, such as aneuploidy or endo reduplication. It follows therefore that the genetic status of the re generated plants is different from that of the parent type. In general terms this genetic instability is undesirable, but there are occasions when a callus stage can be purposely included to diversify the genetic base of the crop.


Micropropagation of Woody Plants

Micropropagation of Woody Plants
Author: M. R. Ahuja
Publisher:
Total Pages: 536
Release: 2014-01-15
Genre:
ISBN: 9789401581172

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Tissue Culture as a Plant Production System for Horticultural Crops

Tissue Culture as a Plant Production System for Horticultural Crops
Author: Richard H. Zimmerman
Publisher: Taylor & Francis
Total Pages: 388
Release: 1986-07-31
Genre: Gardening
ISBN: 9789024733781

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Conference on Tissue Culture as a Plant Production System for Horticultural Crops, Beltsville, MD, October 20-23, 1985


Plants from Test Tubes

Plants from Test Tubes
Author: Lydiane Kyte
Publisher: Timber Press (OR)
Total Pages: 176
Release: 1987
Genre: Science
ISBN:

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Acclaimed as the most practical guide to plant tissue culture, the book is now even better and introduces new developments in biotechnology, such as genetic engineering and cell culture.


Plant Tissue Culture

Plant Tissue Culture
Author: Roberta H. Smith
Publisher: Academic Press
Total Pages: 206
Release: 2013
Genre: Science
ISBN: 0124159206

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Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature


Pathogen and Microbial Contamination Management in Micropropagation

Pathogen and Microbial Contamination Management in Micropropagation
Author: Alan C. Cassells
Publisher: Springer Science & Business Media
Total Pages: 364
Release: 2013-06-29
Genre: Science
ISBN: 9401589518

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This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo.


Somatic Embryogenesis in Woody Plants

Somatic Embryogenesis in Woody Plants
Author: S.M. Jain
Publisher: Springer Science & Business Media
Total Pages: 743
Release: 2013-11-11
Genre: Science
ISBN: 940173030X

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The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.