Microfabricated Multianalyte Sensor Arrays For Metabolite Monitoring PDF Download
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Total Pages | : 36 |
Release | : 2005 |
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Download Microfabricated Multianalyte Sensor Arrays for Metabolite Monitoring Book in PDF, ePub and Kindle
Development of simple and painless techniques of monitoring metabolites like glucose with increased frequency would be beneficial to diabetic patients. Implantable sensors for glucose have been under investigation for nearly three decades, Poor stability and sensitivity of these sensors limit their use in closed-loop delivery. In this work we have taken advantage of silicon micro-fabrication technologies to develop implantable redundant microsensor arrays with glucose oxidase molecules immobilized in photopolymerized and microlithographically patterned films. We have used redox polymers that exchange electrons with glucose oxidase and also form macromolecular networks with these enzymes. The enzymes entrapped in these polymer films and containing biocompatible hydrogels show good stability and sensitivity. Key accomplishments include: a)Successively synthesized an osmium based polycationic redox polymer (POs-Ea) a molecule that is responsible for exchanging electrons with glucose oxidase enzyme; b) Used photolithography to fabricate patterned sensor arrays on flexible plastic substrates (mylar and polyimide); c) Successively crosslinked active glucose oxidase enzyme with redox polymer and biocompatible polyethylene glycol diacrylate hydrogel; d)Used amperometry and cyclic voltammetry to confirm activity of the enzyme and contribution of each sensor array element; e) The enzyme exchanged electrons with redox polymer both entrapped in a hydrogel network.
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Total Pages | : 23 |
Release | : 2006 |
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Download Microfabricated Multianalyte Sensor Arrays for Metabolic Monitoring Book in PDF, ePub and Kindle
Glucose sensor arrays were fabricated on gold electrodes on flexible polyimide sheets by cross-linking glucose oxidase and redox polymer using UV-initiated free radical reaction. Using conventional silicon fabrication methods, five-element array Au microdisks were initially fabricated using mid-UV photolithography. Active glucose oxidase was entrapped in hydrogel by UV-initiated photo polymerization with poly(ethylene glycol) diacrylate or PEG-DA on the array electrodes. The fabricated microarray sensors were individually addressable and with no cross-talk between adjacent array elements as assessed using cyclic voltammetry. We have fabricated an array of glucose sensors on flexible polyimide sheets that exhibit the desired linear response in the biological range. We have also tested the sensors using other electrochemical methods including amperometry and square wave voltammetry (which is known for its background reduction).
Author | : Becky L. Clark |
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Total Pages | : |
Release | : 2007 |
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Download An Implantable Electrochemical Multi-analyte Sensor Array for Metabolite Monitoring Book in PDF, ePub and Kindle
Author | : United States. Congress. Senate. Committee on Appropriations. Subcommittee on Department of Defense |
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Total Pages | : 728 |
Release | : 2005 |
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Download Department of Defense Appropriations Book in PDF, ePub and Kindle
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Release | : 1995 |
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Download Development of Multianalyte Sensor Arrays for Continuous Monitoring of Pollutants Book in PDF, ePub and Kindle
Author | : United States. Congress. Senate. Committee on Appropriations. Subcommittee on Defense |
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Total Pages | : 732 |
Release | : 2005 |
Genre | : History |
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Download Department of Defense Appropriations for Fiscal Year 2006 Book in PDF, ePub and Kindle
Author | : Joachim Wegener |
Publisher | : Springer Nature |
Total Pages | : 286 |
Release | : 2019-11-18 |
Genre | : Science |
ISBN | : 3030324338 |
Download Label-Free Monitoring of Cells in vitro Book in PDF, ePub and Kindle
This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.
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Total Pages | : 17 |
Release | : 1994 |
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Download Production and Preliminary Testing of Multianalyte Imaging Sensor Arrays Book in PDF, ePub and Kindle
This report covers the production and preliminary testing of fiber optic sensors that contain a discrete array of analyte specific sensors on their distal ends. The development of the chemistries associated with this technology is covered elsewhere.
Author | : Jochen Kieninger |
Publisher | : Walter de Gruyter GmbH & Co KG |
Total Pages | : 404 |
Release | : 2022-02-21 |
Genre | : Technology & Engineering |
ISBN | : 3110649756 |
Download Electrochemical Methods for the Micro- and Nanoscale Book in PDF, ePub and Kindle
Are electrochemical methods like asking the crystal ball? Once you read this book about electrochemistry on the micro- and nanoscale, you know it better. This textbook presents the essentials of electrochemical theory, sheds light on the instrumentation, including details on the electronics, and in the second part, discusses a wide variety of classical and advanced methods. The third part of the book covers how to apply the techniques for selected aspects of material science, microfabrication, nanotechnology, MEMS, NEMS, and energy applications. With this book, you will be able to successfully apply the methods in the fields of sensors, neurotechnology, biomedical engineering, and electrochemical energy systems. Undergraduate or Master students can read the book linearly as a comprehensive textbook. For Ph.D. students, postdoctoral researchers as well as for researchers in industry, the book will help by its clear structure to get fast answers from a specific section.
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Total Pages | : 15 |
Release | : 1995 |
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Download Development of the Indicator-photopolymer Chemistries for Multianalyte Sensor Arrays Book in PDF, ePub and Kindle
Remediation of ground and waste water facilities requires the analysis of the pollutants. Multianalyte fiber-optic chemical sensors based on indicators have been developed, with multiple indicators immobilized at the distal end of a single imaging fiber. By coupling the imaging fibers to a charge coupled device detector, one can spatially and spectrally discriminate the multiple sensing sites and hence monitor multiple analyte concentrations simultaneously. This report describes the development of the indicator chemistry and immobilization procedures developed for pH, Al{sup 3+}, and hydrocarbons. A polymer matrix is used to mass transfer the analyte to the indictor.