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| Author | : René F. Masseyeff |
| Publisher | : John Wiley & Sons |
| Total Pages | : 603 |
| Release | : 1993-01-01 |
| Genre | : Immunoassay |
| ISBN | : 9780895739049 |
| Author | : Rene Masseyeff |
| Publisher | : Wiley-Blackwell |
| Total Pages | : 0 |
| Release | : 1993-09-16 |
| Genre | : Medical |
| ISBN | : 9783527279081 |
Volume 3: The latest in the MIA series, which is a remarkable practice-oriented account of immunological analysis. Volumes 1, 2 and 3 provide an extraordinarily comprehensive and up-to-date depiction of methods of immunological analysis. Volume 1 deals predominantly with methodological aspects of immunoassay formats and systems. Volume 2 covers the pre-analytical phase and the production and characterization of reagents. Volume 3 is concerned with the approach to using immunological methods for the analysis of cells and tissues. In the first sections, the methods necessary for the isolation of cells from many different tissues are described, with the emphasis upon human cells. Moreover, the various approaches to the use of antibody-based methods in immunocytochemistry are delineated. Their comparative uses and performance are discussed in critical detail. The last part of the volume considers the ways in which immune cells can be analysed by methods conventionally understood by immunologists, but not necessarily using antibodies. A brief summary of major topics in Volume 3: *preparation of Cells and Tissues * Cell Culture * Cell Fractionation and Purification * Methods of Phenotype Analysis * Immunoanalysis of Cell Function * Analysis of Immune Cell Function.
| Author | : Curtis A. Williams |
| Publisher | : Academic Press |
| Total Pages | : 480 |
| Release | : 2014-05-12 |
| Genre | : Medical |
| ISBN | : 1483222993 |
Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.
| Author | : Rene Masseyeff |
| Publisher | : Wiley-Blackwell |
| Total Pages | : 752 |
| Release | : 1992-12-11 |
| Genre | : Medical |
| ISBN | : 9783527279067 |
Everyone conducting analyses using immunological methods should read METHODS OF IMMUNOLOGICAL ANALYSIS. Now available! Three detailed volumes supplying a complete practice-oriented introduction to immunoanalysis. A set in themselves, they provide an extraordinarily comprehensive and up-to-date depiction of these methods. Both specialists and non-specialists will appreciate the broad scope of this set: It covers a wide area of the life sciences, chemistry and medicine, adjusted to the extensive applicability of immunoanalytical techniques. We at VCH feel that there is a definite need for this indispensable new series. Immunoanalysis is widely practised by analysts whose interests or training are not primarily in immunology. They clearly need the guidance that a good reference work provides. Furthermore, the range of immunochemical methods itself is so broad and its techniques so varied that even specialist immunologists and immunochemists find it difficult to assess different possible analytical approaches. They, too, need the support of an extensive reference work. Save 20% by subscribing to the series by 30 June 1993!
| Author | : Bruce Alberts |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cytology |
| ISBN | : 9780815332183 |
| Author | : Rafael Fernandez-Botran |
| Publisher | : CRC Press |
| Total Pages | : 269 |
| Release | : 2001-06-26 |
| Genre | : Medical |
| ISBN | : 1420041355 |
A step-by-step guide to commonly used procedures, Methods in Cellular Immunology addresses both human and murine models, in addition to such topics as PCR and apoptosis. The basic format of the original version has been maintained, and the goal remains the same: to make it a useful and easy-to-use tool for investigators employing cellular immunolog
| Author | : Heddy Zola |
| Publisher | : CRC Press |
| Total Pages | : 288 |
| Release | : 1990-05-23 |
| Genre | : Medical |
| ISBN | : |
This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.
| Author | : Werner Luttmann |
| Publisher | : Academic Press |
| Total Pages | : 259 |
| Release | : 2006-06-05 |
| Genre | : Medical |
| ISBN | : 0120885441 |
Immunology was written for technical assistants, laboratory workers, students, doctoral candidates and for everyone interested in modern immunological methods.
| Author | : Ivan Lefkovits |
| Publisher | : Academic Press |
| Total Pages | : 492 |
| Release | : 2014-06-28 |
| Genre | : Science |
| ISBN | : 148326999X |
Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.
| Author | : Ivan Lefkovits |
| Publisher | : Elsevier |
| Total Pages | : 506 |
| Release | : 2014-05-12 |
| Genre | : Nature |
| ISBN | : 1483269639 |
Immunological Methods, Volume III provides information pertinent to the fundamental aspects of immunological methods. This book presents recombinant DNA technology as applied to immunology. Organized into 25 chapters, this volume begins with an overview of the major histocompatibility complex. This text then examines the use of monoclonal antibodies in the identification of lymphocyte membrane antigens and explains the use of monoclonal antibodies in histocompatibility typing. Other chapters consider the methods in two-dimensional electrophoretic analysis of proteins and another to lymphokines that support B cells. This book discusses as well the production and expansion of cloned B and T cell lines and hybridomas. The final chapter deals with the sophisticated methodology used with particular animal species, namely, birds, sheep, and amphibians to probe specific immunological questions. This book is a valuable resource for immunologists and investigators with extensive experience in using immunological methods. Protein chemists and biologists will also find this book useful.
