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Diagnostic Bacteriology Protocols

Diagnostic Bacteriology Protocols
Author: Jenny Howard
Publisher: Springer Science & Business Media
Total Pages: 285
Release: 2008-02-03
Genre: Science
ISBN: 1592595332

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Enhanced public awareness of microbiological issues and increased governmental regulation have ensured that microbiology and bacteriology remain important, from the academic laboratory to a broad range of indus trial environments. Since the techniques used in bacteriology vary widely and have advanced rapidly of late, it has become increasingly desirable to gather a collection of up-to-date protocols from a comprehensive range of disciplines into a single volume. Diagnostic Bacteriology Protocols thus aims to provide a wide variety of up-to-date protocols for the identification and differentiation of bacteria from pure or mixed cultures, and for the study of bacteria at the genus through to subspecies levels. In addition, we aim to provide methods covering a range of complexities, some requiring little or no special equipment, others exploiting recent advances in sophisticated instrumentation or novel nucleic acid-based technology. We also present techniques to study a variety of aspects of bacteria—from the features of whole cells and their growth, through their enzyme activities and structural components, to nucleic ac- based analysis.


Methods in Molecular Biology: Diagnostic bacteriology protocols

Methods in Molecular Biology: Diagnostic bacteriology protocols
Author: John M. Walker
Publisher:
Total Pages:
Release: 1984
Genre: Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
ISBN:

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Diagnostic Bacteriology

Diagnostic Bacteriology
Author: Kimberly A. Bishop-Lilly
Publisher: Humana
Total Pages: 264
Release: 2018-08-12
Genre: Science
ISBN: 9781493983759

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This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a wide range of assay types all on the cutting edge of diagnostic bacteriology.


Molecular Bacteriology

Molecular Bacteriology
Author: Springer
Publisher:
Total Pages: 696
Release: 2012-11-27
Genre:
ISBN: 9781468495027

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Diagnostic Bacteriology Protocols

Diagnostic Bacteriology Protocols
Author: Louise O’Connor
Publisher: Humana Press
Total Pages: 226
Release: 2006-04-21
Genre: Science
ISBN: 9781588295941

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The field of bacterial diagnostics has seen unprecedented advances in recent years. The increased need for accurate detection and identification of bacteria in human, animal, food, and environmental samples has fueled the development of new techniques. The field has seen extensive research aided by the information from bacterial genome sequencing projects. Although traditional methods of bacterial detection and identification remain in use in laboratories around the world, there is now a growing trend toward the use of nucleic ac- based diagnostics and alternative biochemically and immunologically based formats. The ultimate goal of all diagnostic tests is the accurate detection, identification, or typing of microorganisms in samples of interest. Although the resulting information is of obvious use in the areas of patient management, animal health, and quality control, it is also of use in monitoring routes of infection and outlining strategies for infection control. There is, therefore, a need to ensure that the information being provided is of the highest standard and that any new technique is capable of delivering this.


Techniques In Molecular Diagnostics

Techniques In Molecular Diagnostics
Author: Kandarpa Kr. Saikai
Publisher: New India Publishing Agency
Total Pages: 8
Release: 2016-09-23
Genre: Science
ISBN: 9385516523

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The book covers from the very basic introduction on molecular biology and diagnostics to the protocols of advanced assays that can be offered by a diagnostic laboratory. There is detailed description on the requirements for setting up a laboratory. The chapter on laboratory mathematics will help the reader in preparation of common laboratory solutions. The book contains basic protocols to perform nucleic acid extraction from different sources. These protocols can be effortlessly applied to isolate high quality DNA and RNA from human blood and tissues. The book is profusely explained with various molecular diagnostic tools which are easy to understand and can be applied in diagnosis of different cancers. This book also presents with examples of biomarker detection protocols for specific cancer type using molecular diagnostic tools which can easily be reproduced. These protocols are very sensitive, cost effective and can be performed to provide faster and accurate diagnosis. The last part of the book deliberates on the quality assurance and quality control strategies of a molecular diagnostic laboratory. Special emphasis is made on specific guidelines to design quality manuals for a molecular diagnostic laboratory


Molecular Bacteriology

Molecular Bacteriology
Author: Neil Woodford
Publisher: Springer Science & Business Media
Total Pages: 704
Release: 1998-04-30
Genre: Medical
ISBN:

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The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .


Molecular Microbiology

Molecular Microbiology
Author: David H. Persing
Publisher: John Wiley & Sons
Total Pages: 835
Release: 2020-07-24
Genre: Science
ISBN: 1555819079

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Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.


Antibiotic Resistance Protocols

Antibiotic Resistance Protocols
Author: Stephen H. Gillespie
Publisher: Springer Nature
Total Pages: 229
Release:
Genre:
ISBN: 1071639811

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