Mass Spectrometry Based Glycoproteomics And Its Clinic Application PDF Download
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Author | : Haojie Lu |
Publisher | : CRC Press |
Total Pages | : 258 |
Release | : 2021-09-21 |
Genre | : Science |
ISBN | : 1000406601 |
Download Mass Spectrometry–Based Glycoproteomics and Its Clinic Application Book in PDF, ePub and Kindle
As one of the most extensive and important protein post-translational modifications, glycosylation plays a vital role in regulating organisms and is associated with various physiological and pathological processes. Recently, researchers have focused on the need to characterize protein glycosylation sites, structures, and their degree of modification, to better understand their biological functions while also looking for potential biomarkers for diagnosis and treatment of disease. Mass spectrometry (MS) is one of the most powerful tools used to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more techniques have evolved and contribute to understanding the structure and function of glycoproteins and glycans. This book reviews advancements achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment; as well as qualitative, quantitative, and data analysis, together with their clinical applications. Significant examples are discussed to illustrate the principles, laboratory protocols, and advice for key implementation to ensure successful results. Mass Spectrometry–Based Glycoproteomics and Its Clinic Application will serve as a valuable resource to elucidate new techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine. Editor: Haojie Lu is a professor at Fudan University, specializing in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.
Author | : Haojie Lu |
Publisher | : CRC Press |
Total Pages | : 280 |
Release | : 2021 |
Genre | : Science |
ISBN | : 9781003185833 |
Download Mass Spectrometry-Based Glycoproteomics and Its Clinic Application Book in PDF, ePub and Kindle
As one of the most extensive and important protein post-translational modifications, glycosylation plays very important roles in the organism and is associated with different physiological and pathological processes. Therefore, there has been increasing attention and need to characterize protein glycosylation sites, structures and their modification degree, in order to understand well their biological functions or look for potential biomarkers for disease diagnosis and treatment.Mass spectrometry (MS) is one of the most powerful tools to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more and more techniques have appeared and contribute to understanding the structure and function of glycoproteins and glycans. Therefore, this book demonstrates the progress that has been achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment, qualitative analysis, quantitative analysis and data analysis, together with their clinical applications. And some significant examples will be discussed to illustrate the principles, laboratory protocols and key implementation advice to ensure successful results.This book will serve as a valuable resource to appropriate techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine, and so on.
Author | : Rudolf Bock |
Publisher | : Walter de Gruyter GmbH & Co KG |
Total Pages | : 642 |
Release | : 2023-07-24 |
Genre | : Science |
ISBN | : 3111182061 |
Download Separation Techniques in Analytical Chemistry Book in PDF, ePub and Kindle
The separation of a mixture into its individual components is one of the most fundamental procedures in analytical and industrial chemistry. This classic book in analytical chemistry provides a comprehensive yet systematic outline of all known separation methods. Through its detailed treatment of the basic principles of separation possibilities, it not only covers what is currently known, but also represents a treasure trove of methods that are still awaiting further development. It is clearly structured and contains interesting examples, further reading and a detailed index. An indispensable book for advanced students of natural sciences (chemistry, biochemistry, food chemistry, pharmacy, clinical chemistry, environmental sciences) and technology (chemical engineering, chemical-physical measurement & biotechnology), as well as teachers of these disciplines.
Author | : Sumit Kumar Singh |
Publisher | : Springer |
Total Pages | : 0 |
Release | : 2024-10-02 |
Genre | : Medical |
ISBN | : 9789819750443 |
Download Protein Biomarkers: Discovery and Applications in Clinical Diagnostics Book in PDF, ePub and Kindle
This book comprehensively reviews the fundamentals of biomarker discovery using mass-spectrometry-based proteomic and glycoproteomic methods. It also provides case studies of proteomic and glycoproteomic molecular signatures to illustrate the design and use of various mass spectrometry modes such as selected reaction monitoring (SRM), parallel reaction monitoring (PRM), and sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). In turn, the book presents the challenges of the proteomic methods in biomarker discovery and approaches for overcoming these challenges by extending the performance by improving the sensitivity, throughput, and selectivity of the method. It also addresses the bottleneck of deriving useful biological interpretations from large multi-dimensional datasets emanating from these experiments by discussing data analysis pipelines and appropriate statistical analysis. Finally, a detailed discussion on the design, characterization, and application of protein/glycoprotein-based biosensors for clinical diagnostics is discussed. The book is intended to serve as a reliable resource for the students and researchers working in the area of biomarker discovery and validation using mass spectrometry-based methods and their subsequent applications for biosensor design.
Author | : Mary S. Lipton |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2014-11-27 |
Genre | : Science |
ISBN | : 9781627037969 |
Download Mass Spectrometry of Proteins and Peptides Book in PDF, ePub and Kindle
When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.
Author | : Jia Zhao |
Publisher | : |
Total Pages | : 374 |
Release | : 2007 |
Genre | : |
ISBN | : |
Download Development and Application of Comparative Proteomics and Glycoproteomics Approaches Based on Liquid Phase Separation and Mass Spectrometry Book in PDF, ePub and Kindle
Author | : Jennifer J. Kohler |
Publisher | : Humana Press |
Total Pages | : 352 |
Release | : 2016-05-01 |
Genre | : Science |
ISBN | : 9781493962037 |
Download Mass Spectrometry of Glycoproteins Book in PDF, ePub and Kindle
This comprehensive new resource in Springer s Methods in Molecular Biology series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry."
Author | : Antonia Vlahou |
Publisher | : Springer Science & Business Media |
Total Pages | : 401 |
Release | : 2008-01-18 |
Genre | : Science |
ISBN | : 158829837X |
Download Clinical Proteomics Book in PDF, ePub and Kindle
In this book, a select group of researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. The book integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. Experimental approaches are thoroughly detailed and explained through a step-by-step instructional format that ensures successful results.
Author | : |
Publisher | : |
Total Pages | : |
Release | : 2009 |
Genre | : |
ISBN | : |
Download Clinical Applications of Mass Spectrometry Book in PDF, ePub and Kindle
Author | : Haleem J. Issaq |
Publisher | : Academic Press |
Total Pages | : 504 |
Release | : 2019-10-24 |
Genre | : Science |
ISBN | : 0128197889 |
Download Proteomic and Metabolomic Approaches to Biomarker Discovery Book in PDF, ePub and Kindle
Proteomic and Metabolomic Approaches to Biomarker Discovery, Second Edition covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods and presents a standard operating procedure for sample selection, preparation and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. This second edition has been fully updated and revised to address recent advances in MS and NMR instrumentation, high-field NMR, proteomics and metabolomics for biomarker validation, clinical assays of biomarkers and clinical MS and NMR, identifying microRNAs and autoantibodies as biomarkers, MRM-MS assay development, top-down MS, glycosylation-based serum biomarkers, cell surface proteins in biomarker discovery, lipodomics for cancer biomarker discovery, and strategies to design studies to identify predictive biomarkers in cancer research. Addresses the full range of proteomic and metabolomic methods and technologies used for biomarker discovery and validation Covers all steps involved in biomarker discovery, from study design to study execution Serves as a vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals and graduate students