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Lumi-ImagerTM F1

Lumi-ImagerTM F1
Author: Onno Bakker
Publisher: Springer Science & Business Media
Total Pages: 57
Release: 2013-04-17
Genre: Science
ISBN: 3662084317

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The instrument Lumi-Imager was developed for the detection and accurate quantification of chemiluminescent signals on blots and in microtiter plates. It contains a cooled CCD camera, a specially developed lens system and software. In addition to luminescence, Lumi-Imager F1 can also detect and quantify UV-excited fluorescent signals in gels. Here we describe the components and features of this instrument as well as some of its applications.


Lumi-Imager F1

Lumi-Imager F1
Author: Onno Bakker
Publisher:
Total Pages: 60
Release: 2014-01-15
Genre:
ISBN: 9783662084328

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Development of Methods for Directed and Structured Immobilization of Biomolecules

Development of Methods for Directed and Structured Immobilization of Biomolecules
Author: Björn Waterkotte
Publisher: Cuvillier Verlag
Total Pages: 252
Release: 2013-11-07
Genre: Science
ISBN: 3736945329

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Surface immobilized molecules play a crucial role in applied as well as in basic research. They can be found in DNA or protein-array as well as in cell-migration or biochemical interaction experiments. Especially for proteins the immobilization can critically affect molecule accessibility and activity. In this work two essential aspects of protein immobilization were investigated: the identification of suitable surface functionalization by two array approaches and the generation of laterally structured gray scale protein patterns by projection lithography. In proof-of-concept experiments a new strategy to create an array of different, neighboring functionalizations on the same surface was successfully tested. In "mechanical protection" inert plungers were applied to protect distinct surface areas from the surface functionalization reagents and allowed a selective modification of individual regions in serial reactions. In a particle based alternative approach a high spot density surface array was fabricated using functionalized, polyvinyl acetate, melamine and silicon oxide-based particles. The in situ encoded bead-based array (IEBA) allowed the study of immobilization conditions and the effect of protein adhesion. For the reliable and fast analysis of array-images with several thousand individual particles a custom open-source evaluation software was developed. To create protein patterns a custom-built projection lithography device was utilized to generate high definition protein patterns. Patterning conditions were optimized with respect to deposition speed and signal to noise ratio while retaining a biocompatible process flow. The established technology provides protein patterns with great control over the deposited surface density, patterns were transferred within seconds to minutes to functionalized glass slides. To obtain three-dimensional, surface-functionalized surfaces this approach was transferred to thin polymer films. The substrates were demonstrated to be biocompatible with mouse fibroblasts. These surfaces allow a novel level of control addressing both the chemical surrounding and the shape of a cell microenvironment.


Imaging Optics

Imaging Optics
Author: Joseph Braat
Publisher: Cambridge University Press
Total Pages: 987
Release: 2019-05-02
Genre: Medical
ISBN: 1108428088

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This comprehensive and self-contained text for researchers and professionals presents a detailed account of optical imaging from the viewpoint of both ray and wave optics.


Statistical Parametric Mapping: The Analysis of Functional Brain Images

Statistical Parametric Mapping: The Analysis of Functional Brain Images
Author: William D. Penny
Publisher: Elsevier
Total Pages: 689
Release: 2011-04-28
Genre: Psychology
ISBN: 0080466508

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In an age where the amount of data collected from brain imaging is increasing constantly, it is of critical importance to analyse those data within an accepted framework to ensure proper integration and comparison of the information collected. This book describes the ideas and procedures that underlie the analysis of signals produced by the brain. The aim is to understand how the brain works, in terms of its functional architecture and dynamics. This book provides the background and methodology for the analysis of all types of brain imaging data, from functional magnetic resonance imaging to magnetoencephalography. Critically, Statistical Parametric Mapping provides a widely accepted conceptual framework which allows treatment of all these different modalities. This rests on an understanding of the brain's functional anatomy and the way that measured signals are caused experimentally. The book takes the reader from the basic concepts underlying the analysis of neuroimaging data to cutting edge approaches that would be difficult to find in any other source. Critically, the material is presented in an incremental way so that the reader can understand the precedents for each new development. This book will be particularly useful to neuroscientists engaged in any form of brain mapping; who have to contend with the real-world problems of data analysis and understanding the techniques they are using. It is primarily a scientific treatment and a didactic introduction to the analysis of brain imaging data. It can be used as both a textbook for students and scientists starting to use the techniques, as well as a reference for practicing neuroscientists. The book also serves as a companion to the software packages that have been developed for brain imaging data analysis. An essential reference and companion for users of the SPM software Provides a complete description of the concepts and procedures entailed by the analysis of brain images Offers full didactic treatment of the basic mathematics behind the analysis of brain imaging data Stands as a compendium of all the advances in neuroimaging data analysis over the past decade Adopts an easy to understand and incremental approach that takes the reader from basic statistics to state of the art approaches such as Variational Bayes Structured treatment of data analysis issues that links different modalities and models Includes a series of appendices and tutorial-style chapters that makes even the most sophisticated approaches accessible


Occultism, Witchcraft, and Cultural Fashions

Occultism, Witchcraft, and Cultural Fashions
Author: Mircea Eliade
Publisher: University of Chicago Press
Total Pages: 161
Release: 1978-03-15
Genre: Body, Mind & Spirit
ISBN: 0226203921

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Six essays on a variety of interrelated subjects.


Proceedings

Proceedings
Author: Hugo Kasper
Publisher:
Total Pages: 542
Release: 1968
Genre: Photogrammetry
ISBN:

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Scientific Photography and Applied Imaging

Scientific Photography and Applied Imaging
Author: Sidney Ray
Publisher: CRC Press
Total Pages: 622
Release: 1999-08-02
Genre: Photography
ISBN: 1136094377

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WINNER OF THE 2001 KRASZNA-KRAUSZ PHOTOGRAPHY BOOK AWARD (Technical Photography category) The only definitive book to fully encompass the use of photography and imaging as tools in science, technology and medicine. It describes in one single volume the basic theory, techniques, materials, special equipment and applications for a wide variety of uses of photography, including: close up photography and photomacrography to spectral recording, surveillance systems, radiography and micro-imaging. This extensively illustrated photography 'bible' contains all the information you need, whether you are a scientist wishing to use photography for a specialist application, a professional needing to extend technical expertise, or a student wanting to broaden your knowledge of the applications of photography. The contents are arranged in three sections: · General Section, detailing the elements of the image capture process · Major Applications, describing the major applications of imaging · Specialist Applications, presenting an eclectic selection of more specialised but increasingly important applications Each subject is introduced with an outline of its development and contemporary importance, followed by explanations of essential theory and an overview of techniques and equipment. Mathematics is only used where necessary. Numerous applications and case studies are described. Comprehensive bibliographies and references are provided for further study.


Signal Processing and Performance Analysis for Imaging Systems

Signal Processing and Performance Analysis for Imaging Systems
Author: S. Susan Young
Publisher: Artech House
Total Pages: 323
Release: 2008
Genre: Computers
ISBN: 1596932880

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This book presents today's most powerful signal processing techniques together with methods for assessing imaging system performance when each of these techniques is applied. This multi-use book helps you make the most of sensor hardware through software enhancement, and evaluate system and algorithm performance. You also learn how to make the best hardware/software decisions in developing the next-generation of image acquisition and analysis systems.