Localized Surface Plasmon Resonance Spectroscopy Of Gold And Silver Nanoparticles And Plasmon Enhanced Flurescence PDF Download
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Author | : Elizabeth Anne Vokac |
Publisher | : |
Total Pages | : 100 |
Release | : 2011 |
Genre | : |
ISBN | : |
Download Localized Surface Plasmon Resonance Spectroscopy of Gold and Silver Nanoparticles and Plasmon Enhanced Flurescence Book in PDF, ePub and Kindle
This thesis presents spectroscopic studies of metallic nanoparticle localized surface plasmons and plasmon enhanced fluorescence. We investigated the dielectric sensitivity of silver nanoprisms to an external electric field and gold nanorods to the formation of a self-assembled surface monolayer. Dark field microscopy was used to image plasmonic scattering from single nanoparticles, and a liquid crystal tunable filter was used to construct corresponding spectra. The plasmon resonances of silver nanoprisms displayed both reversible red shifts and irreversible blue shifts along with drastic intensity changes upon exposure to an applied bias. The plasmon resonances of gold nanorods showed sensitivity to the presence of alkanethiol molecules adhered to the particle surface by a moderate red shift. An increase in the effective external dielectric caused a shift toward longer wavelengths. We imaged plasmon enhanced fluorescence in order to optimize experimental parameters for a developing project that can characterize nanoparticle structure on sub-wavelength dimensions. Preliminary controls were performed to account for the effect of O2 plasma treatment, solvent and alkanethiol monolayer formation on surface plasmon resonances. We found that O2 plasma treatment for different time intervals did not result in a plasmon shift compared to untreated nanoparticles exposed to N2; however when exposed to solvent the surface plasmons of the treated particles shifted five times as far toward the red. Interestingly, the solvent effect only resulted in a plasmon shift when the particles were N2 dried after solvent incubation. Gold nanorods incubated in ethanol showed no wavelength maximum shift in pure solvent over time, but shifted moderately to the red after incubation in a solution of alkanethiol molecules. Conditions for the plasmon enhanced fluorescence study were optimized using a dye conjugate of the same alkanethiol molecule used previously by formation from solution in a monolayer on the gold nanorod surface. The appropriate synthesis for dye functionalization, molecular concentrations, solvents and optical settings were determined.
Author | : Chris D. Geddes |
Publisher | : John Wiley & Sons |
Total Pages | : 655 |
Release | : 2010-06-22 |
Genre | : Science |
ISBN | : 0470642785 |
Download Metal-Enhanced Fluorescence Book in PDF, ePub and Kindle
Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Author | : Chris D. Geddes |
Publisher | : John Wiley & Sons |
Total Pages | : 339 |
Release | : 2017-04-10 |
Genre | : Science |
ISBN | : 1118027930 |
Download Surface Plasmon Enhanced, Coupled and Controlled Fluorescence Book in PDF, ePub and Kindle
Explains the principles and current thinking behind plasmon enhanced Fluorescence Describes the current developments in Surface Plasmon Enhanced, Coupled and Controlled Fluorescence Details methods used to understand solar energy conversion, detect and quantify DNA more quickly and accurately, and enhance the timeliness and accuracy of digital immunoassays Contains contributions by the world’s leading scientists in the area of fluorescence and plasmonics Describes detailed experimental procedures for developing both surfaces and nanoparticles for applications in metal-enhanced fluorescence
Author | : Volodymyr I. Chegel |
Publisher | : CRC Press |
Total Pages | : 420 |
Release | : 2020-11-18 |
Genre | : Technology & Engineering |
ISBN | : 1000020959 |
Download Molecular Plasmonics Book in PDF, ePub and Kindle
This book summarizes the results of studies of molecules and molecular complexes using techniques based on surface plasmon resonance (SPR) in a novel scientific direction called molecular plasmonics. It presents the current state of investigations in the field of molecular plasmonics and discusses its two main physical phenomena: surface plasmon–polariton resonance (SPPR) and localized SPR (LSPR). Among the mathematical methods for the calculation of plasmonic systems response, the book emphasizes models based on the transfer-matrix method, Green function formalism, Mie scattering theory, and numerical methods. It considers the possibilities of the SPPR technique for registering conformational changes, surface plasmon–mediated photopolymerization, electrochemical processes, as well as reversible optoelectronic and physicochemical properties during investigation of molecular systems. It describes applications of the LSPR method, including creation of metamaterials, surface-enhanced fluorescence, and bio- and chemosensing using noble metal nanoparticles in colloidal, array, and composite polymeric film formats. It also highlights the development and applications of plasmonic nanochips.
Author | : Igor Linkov |
Publisher | : Springer Science & Business Media |
Total Pages | : 459 |
Release | : 2008-12-26 |
Genre | : Technology & Engineering |
ISBN | : 1402094914 |
Download Nanomaterials Book in PDF, ePub and Kindle
Many potential questions regarding the risks associated with the development and use of wide-ranging technologies enabled through engineered nanomaterials. For example, with over 600 consumer products available globally, what information exists that describes their risk to human health and the environment? What en- neering or use controls can be deployed to minimize the potential environmental health and safety impacts of nanomaterials throughout the manufacturing and product lifecycles? How can the potential environmental and health benefits of nanotechnology be realized and maximized? The idea for this book was conceived at the NATO Advanced Research Workshop (ARW) on “Nanomaterials: Environmental Risks and Benefits and Emerging Consumer Products. ” This meeting – held in Algarve, Portugal, in April 2008 – started with building a foundation to harmonize risks and benefits associated with nanomaterials to develop risk management approaches and policies. More than 70 experts, from 19 countries, in the fields of risk assessment, decision-analysis, and security discussed the current state-of-knowledge with regard to nanomaterial risk and benefits. The discussion focused on the adequacy of available risk assessment tools to guide nanomaterial applications in industry and risk governance. The workshop had five primary purposes: Describe the potential benefits of nanotechnology enabled commercial products. Identify and describe what is known about environmental and human health risks of nanomaterials and approaches to assess their safety. Assess the suitability of multicriteria decision analysis for reconciling the benefits and risks of nanotechnology.
Author | : Ariel Rodrigo Guerrero Hernandez |
Publisher | : |
Total Pages | : |
Release | : |
Genre | : |
ISBN | : |
Download Plasmon-Enhanced Raman and Fluorescence Spectroscopy with Gold and Silver Nanoparticles Book in PDF, ePub and Kindle
Author | : Alexandre Dmitriev |
Publisher | : Springer Science & Business Media |
Total Pages | : 394 |
Release | : 2012-07-31 |
Genre | : Science |
ISBN | : 1461439337 |
Download Nanoplasmonic Sensors Book in PDF, ePub and Kindle
This book is a compendium of the finest research in nanoplasmonic sensing done around the world in the last decade. It describes basic theoretical considerations of nanoplasmons in the dielectric environment, gives examples of the multitude of applications of nanoplasmonics in biomedical and chemical sensing, and provides an overview of future trends in optical and non-optical nanoplasmonic sensing. Specifically, readers are guided through both the fundamentals and the latest research in the two major fields nanoplasmonic sensing is applied to – bio- and chemo-sensing – then given the state-of-the-art recipes used in nanoplasmonic sensing research.
Author | : Mark Stauffer |
Publisher | : BoD – Books on Demand |
Total Pages | : 442 |
Release | : 2016-10-05 |
Genre | : Science |
ISBN | : 9535126806 |
Download Applications of Molecular Spectroscopy to Current Research in the Chemical and Biological Sciences Book in PDF, ePub and Kindle
The goal of this book is to present an overview of applications of molecular spectroscopy to investigations in organic and inorganic materials, foodstuffs, biosamples and biomedicine, and novel characterization and quantitation methods. This text is a compilation of selected research articles and reviews covering current efforts in various applications of molecular spectroscopy. Sections 1 and 2 deal, respectively, with spectroscopic studies of inorganic and organic materials. Section 3 provides applications of molecular spectroscopy to biosamples and biomedicine. Section 4 explores spectroscopic characterization and quantitation of foods and beverages. Lastly, Section 5 presents research on novel spectroscopic methodologies. Overall, this book should be a great source of scientific information for anyone involved in characterization, quantitation, and method development.
Author | : Lev Dykman |
Publisher | : CRC Press |
Total Pages | : 365 |
Release | : 2017-12-12 |
Genre | : Medical |
ISBN | : 1351360477 |
Download Gold Nanoparticles in Biomedical Applications Book in PDF, ePub and Kindle
This book discusses fabrication of functionalized gold nanoparticles (GNPs) and multifunctional nanocomposites, their optical properties, and applications in biological studies. This is the very first book of its kind to comprehensively discuss published data on in vitro and in vivo biodistribution, toxicity, and uptake of GNP by mammalian cells providing a systematization of data over the GNP types and parameters, their surface functionalization, animal and cell models. As distinct from other related books, Gold Nanoparticles in Biomedical Applications discusses the immunological properties of GNPs and summarizes their applications as an antigen carrier and adjuvant in immunization for the preparation of antibodies in vivo. Although the potential of GNPs in nanobiotechnology has been recognized for the past decade, new insights into the unique properties of multifunctional nanostructures have recently emerged. With these developments in mind, this book unites ground breaking experimental data with a discussion of hybrid nanoparticle systems that combine different nanomaterials to create multifunctional structures. These novel hybrids constitute the material basis of theranostics, bringing together the advanced properties of functionalized GNPs and composites into a single multifunctional nanostructure with simultaneous diagnostic and therapeutic functions. Such nanohybrids can be physically and chemically tailored for a particular organ, disease, and patient thus making personalized medicine available.
Author | : Amanda Jo Haes |
Publisher | : |
Total Pages | : |
Release | : 2004 |
Genre | : |
ISBN | : |
Download Localized Surface Plasmon Resonance Spectroscopy for Fundamental Studies of Nanoparticle Optics and Applications to Biosensors Book in PDF, ePub and Kindle
This work reveals that triangular silver nanoparticles have remarkable optical properties and that the enhanced sensitivity of their nanoenvironment can be used as a new class of optical sensors using localized surface plasmon resonance (LSPR) spectroscopy. The contents of thesis can be divided into two main subject areas: (1) the characterization and optimization of nanoparticles for the basic understanding of their optical properties and (2) the development, testing, and theoretical understanding of model biological assays and nonmodel assays for disease diagnosis. In the first half of this work, an improved understanding of the physical characteristics of noble metal nanoparticles is explored. Specifically, the realization that the sensitivity of these nanoparticles can be optimized for a given adsorbate is revealed. First, it is demonstrated that the short range (viz., 0--3 nm) distance dependence of the electromagnetic fields that surround these nanoparticles can be systematically tuned by changing their composition, size, and structure. Second, multilayer shells are assembled onto surface-confined noble metal nanoparticles to study the long range distance dependence. It is shown that this dependence (viz., 3--35 nm) is non-linear and has a tunable sensing range. Finally, methods to enhance the nanosensor's response based on interactions between the nanoparticle's plasmon resonance and the adsorbate's molecular resonance is revealed. In the remainder sections, we explore the use of these nanoparticles as biological sensors. First, the model system of biotin/streptavidin is confirmed to be highly specific with low nonspecific influences. Next, the demonstration of an immunoassay (biotin/anti-biotin) is performed. Finally, the LSPR nanosensor is shown to be a promising platform for the possible mechanistic understanding of Alzheimer's disease.