Label Free Dna Imaging In Vivo With Stimulated Raman Scattering Microscopy PDF Download
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Total Pages | : 6 |
Release | : 2015 |
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Download Label-free DNA Imaging in Vivo with Stimulated Raman Scattering Microscopy Book in PDF, ePub and Kindle
Label-free DNA imaging is highly desirable in biology and medicine to perform live imaging without affecting cell function and to obtain instant histological tissue examination during surgical procedures. Here we show a label-free DNA imaging method with stimulated Raman scattering (SRS) microscopy for visualization of the cell nuclei in live animals and intact fresh human tissues with subcellular resolution. Relying on the distinct Raman spectral features of the carbon-hydrogen bonds in DNA, the distribution of DNA is retrieved from the strong background of proteins and lipids by linear decomposition of SRS images at three optimally selected Raman shifts. Based on changes on DNA condensation in the nucleus, we were able to capture chromosome dynamics during cell division both in vitro and in vivo. We tracked mouse skin cell proliferation, induced by drug treatment, through in vivo counting of the mitotic rate. Moreover, we demonstrated a label-free histology method for human skin cancer diagnosis that provides comparable results to other conventional tissue staining methods such as H & E. In conclusion, our approach exhibits higher sensitivity than SRS imaging of DNA in the fingerprint spectral region. Compared with spontaneous Raman imaging of DNA, our approach is three orders of magnitude faster, allowing both chromatin dynamic studies and label-free optical histology in real time.
Author | : Ji-Xin Cheng |
Publisher | : Elsevier |
Total Pages | : 612 |
Release | : 2021-12-04 |
Genre | : Science |
ISBN | : 0323903371 |
Download Stimulated Raman Scattering Microscopy Book in PDF, ePub and Kindle
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Author | : Ji-Xin Cheng |
Publisher | : CRC Press |
Total Pages | : 603 |
Release | : 2016-04-19 |
Genre | : Science |
ISBN | : 1439867666 |
Download Coherent Raman Scattering Microscopy Book in PDF, ePub and Kindle
The First Book on CRS MicroscopyCompared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Sc
Author | : Mei Tian |
Publisher | : Elsevier |
Total Pages | : 408 |
Release | : 2024-06-25 |
Genre | : Science |
ISBN | : 0323952240 |
Download Transpathology Book in PDF, ePub and Kindle
Transpathology: Molecular Imaging-Based Pathology is a multidisciplinary reference on molecular imaging and pathology. The book is intended for professionals in the fields of molecular imaging, nuclear medicine, radiology, and pathology as well as students and clinical residents. The book describes the importance of non-invasive diagnosis-based precision medicine and presents a detailed description of current transpathological approaches in different aspects essential for the future development of precision medicine. It’s molecular imaging approach to experimental research and clinical practice will drive the field forward and improve research outcomes. Introduces a new concept of molecular imaging-guided precise biopsy Links in vivo and ex vivo information at various scales by using multi-modality imaging technologies Integrates future technologies for the non-invasive cross-validation of underlying mechanisms
Author | : Mengtao Sun |
Publisher | : Springer Nature |
Total Pages | : 125 |
Release | : |
Genre | : |
ISBN | : 9819936373 |
Download Linear and Nonlinear Optical Spectroscopy and Microscopy Book in PDF, ePub and Kindle
Author | : Shoogo Ueno |
Publisher | : CRC Press |
Total Pages | : 273 |
Release | : 2020-05-26 |
Genre | : Medical |
ISBN | : 0429536569 |
Download Bioimaging Book in PDF, ePub and Kindle
Bioimaging: Imaging by Light and Electromagnetics in Medicine and Biology explores new horizons in biomedical imaging and sensing technologies, from the molecular level to the human brain. It explores the most up-to-date information on new medical imaging techniques, such as the detection and imaging of cancer and brain diseases. This book also provides new tools for brain research and cognitive neurosciences based on new imaging techniques. Edited by Professor Shoogo Ueno, who has been leading the field of biomedical imaging for 40 years, it is an ideal reference book for graduate and undergraduate students and researchers in medicine and medical physics who are looking for an authoritative treatise on this expanding discipline of imaging and sensing in medicine and biology. Features: Provides step-by-step explanations of biochemical and physical principles in biomedical imaging Covers state-of-the art equipment and cutting-edge methodologies used in biomedical imaging Serves a broad spectrum of readers due to the interdisciplinary topic and approach Shoogo Ueno, Ph.D, is a professor emeritus of the University of Tokyo, Tokyo, Japan. His research interests include biomedical imaging and bioelectromagnetics, particularly in brain mapping and neuroimaging, transcranial magnetic stimulation (TMS), and magnetic resonance imaging (MRI). He was the President of the Bioelectromagnetics Society, BEMS (2003-2004) and the Chairman of the Commission K on Electromagnetics in Biology and Medicine of the International Union of Radio Science, URSI (2000-2003). He was named the IEEE Magnetics Society Distinguished Lecturer during 2010 and received the d’Arsonval Medal from the Bioelectromagnetics Society in 2010.
Author | : Zhenxi Zhang |
Publisher | : John Wiley & Sons |
Total Pages | : 309 |
Release | : 2023-02-06 |
Genre | : Science |
ISBN | : 3527823549 |
Download Biomedical Photonic Technologies Book in PDF, ePub and Kindle
Biomedical Photonic Technologies A state-of-the-art examination of biomedical photonic research, technologies, and applications In Biomedical Photonic Technologies, a team of distinguished researchers delivers a methodical inquiry and evaluation of the latest developments in the field of biomedical photonics, with a focus on novel technologies, including optical microscopy, optical coherence tomography, fluorescence imaging-guided surgery, photodynamic therapy dosimetry, and optical theranostic technologies. Each discussion of individual technologies includes examples of their contemporary application in areas like cancer therapy and drug delivery. Readers will discover the major research advancements in biomedical photonics from the last 20 years, ascertaining the basic principles of formation, development, and derivation of biomedical photonics phenomena at a variety of scales. Readers will also find: A thorough introduction to advanced wide-field fluorescent microscopy for biomedicine Comprehensive explorations of fluorescence resonance energy transfer and optical coherence tomography for structural and functional imaging Practical exploration of coherent Raman scattering microscopy and biomedical applications, as well as fluorescence image-guided surgery Complete analyses of enhanced photodynamic therapy, optogenetics, and optical theranostics employing gold nanoparticles Perfect for biophysicists and applied physicists, Biomedical Photonic Technologies will also benefit bioengineers and biotechnologists in academia and in industry.
Author | : Arnaud Zoubir |
Publisher | : Springer |
Total Pages | : 388 |
Release | : 2012-07-27 |
Genre | : Science |
ISBN | : 3642282520 |
Download Raman Imaging Book in PDF, ePub and Kindle
Raman imaging has long been used to probe the chemical nature of a sample, providing information on molecular orientation, symmetry and structure with sub-micron spatial resolution. Recent technical developments have pushed the limits of micro-Raman microscopy, enabling the acquisition of Raman spectra with unprecedented speed, and opening a pathway to fast chemical imaging for many applications from material science and semiconductors to pharmaceutical drug development and cell biology, and even art and forensic science. The promise of tip-enhanced raman spectroscopy (TERS) and near-field techniques is pushing the envelope even further by breaking the limit of diffraction and enabling nano-Raman microscopy.
Author | : Jan Toporski |
Publisher | : Springer |
Total Pages | : 603 |
Release | : 2018-03-01 |
Genre | : Science |
ISBN | : 3319753800 |
Download Confocal Raman Microscopy Book in PDF, ePub and Kindle
This second edition provides a cutting-edge overview of physical, technical and scientific aspects related to the widely used analytical method of confocal Raman microscopy. The book includes expanded background information and adds insights into how confocal Raman microscopy, especially 3D Raman imaging, can be integrated with other methods to produce a variety of correlative microscopy combinations. The benefits are then demonstrated and supported by numerous examples from the fields of materials science, 2D materials, the life sciences, pharmaceutical research and development, as well as the geosciences.
Author | : Wenfeng Xia |
Publisher | : Springer Nature |
Total Pages | : 608 |
Release | : |
Genre | : |
ISBN | : 3031614119 |
Download Biomedical Photoacoustics Book in PDF, ePub and Kindle