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| Author | : Nicolas L. Taylor |
| Publisher | : Methods in Molecular Biology |
| Total Pages | : 352 |
| Release | : 2018-10-26 |
| Genre | : Science |
| ISBN | : 9781493982288 |
| Author | : Paul R. Pryor |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2015 |
| Genre | : Cell fractionation |
| ISBN | : 9781621820420 |
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 495 |
| Release | : 1997-05-06 |
| Genre | : Technology & Engineering |
| ISBN | : 0080561799 |
Advances in Botanical Research is a multi-volume publication that brings together reviews by recognized experts on subjects of importance to those involved in botanical research. For more than thirty years, Advances in Botanical Research has earned a reputation for excellence in the field. For those working on plant pathology, Advances in Plant Pathology has also carved a niche in the plant sciences during its decade of publication.Academic Press has merged Advances in Plant Pathology into Advances in Botanical Research. The plant science community will find that the merger of these two serials will provide one comprehensive resource for the field. To ensure complete coverage, John Andrews and Inez Tommerup, the editors of Advances in Plant Pathology, have joined the editorial board of the new series, which will include equal coverage of plant pathology and botany in both thematic and mixed volumes. The first few volumes of the new series will be slanted toward botany or plant pathology; however, future eclectic volumes will be fully integrated.The resulting synergy of these two serials greatly benefits the plant science community by providing a more comprehensive resource under one roof. The joint aim is to continue to include the very best articles, thereby maintaining the status of a high impact factor review series.
| Author | : Eric Reid |
| Publisher | : |
| Total Pages | : 256 |
| Release | : 1979 |
| Genre | : Science |
| ISBN | : |
| Author | : Zoë A. Popper |
| Publisher | : Lulu.com |
| Total Pages | : 324 |
| Release | : |
| Genre | : |
| ISBN | : 0359580998 |
| Author | : Stanton Gelvin |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 138 |
| Release | : 2013-11-11 |
| Genre | : Science |
| ISBN | : 940115242X |
| Author | : C Hawes |
| Publisher | : Academic Press |
| Total Pages | : 477 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323148980 |
Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
| Author | : Robert E. Farrell Jr. |
| Publisher | : Elsevier |
| Total Pages | : 794 |
| Release | : 2010-07-22 |
| Genre | : Science |
| ISBN | : 0080454763 |
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
| Author | : Nicholas C. Carpita |
| Publisher | : Springer |
| Total Pages | : 333 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 9401006687 |
This work is a comprehensive collection of articles that cover aspects of cell wall research in the genomic era. Some 2500 genes are involved in some way in wall biogenesis and turnover, from generation of substrates, to polysaccharide and lignin synthesis, assembly, and rearrangement in the wall. Although a great number of genes and gene families remain to be characterized, this issue provides a census of the genes that have been discovered so far. The articles comprising this issue not only illustrate the enormous progress made in identifying the wealth of wall-related genes but they also show the future directions and how far we have to go. As cell walls are an enormously important source of raw material, we anticipate that cell-wall-related genes are of significant economic importance. Examples include the modification of pectin-cross-linking or cell-cell adhesion to increase shelf life of fruits and vegetables, the enhancement of dietary fiber contents of cereals, the improvement of yield and quality of fibers, and the relative allocation of carbon to wall biomass for use as biofuels. The book is intended for academic and professional scientists working in the area of plant biology as well as material chemists and engineers, and food scientists who define new ways to use cell walls.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 238 |
| Release | : 2003-03-19 |
| Genre | : Science |
| ISBN | : 0309168392 |
Chemistry and chemical engineering have changed significantly in the last decade. They have broadened their scopeâ€"into biology, nanotechnology, materials science, computation, and advanced methods of process systems engineering and controlâ€"so much that the programs in most chemistry and chemical engineering departments now barely resemble the classical notion of chemistry. Beyond the Molecular Frontier brings together research, discovery, and invention across the entire spectrum of the chemical sciencesâ€"from fundamental, molecular-level chemistry to large-scale chemical processing technology. This reflects the way the field has evolved, the synergy at universities between research and education in chemistry and chemical engineering, and the way chemists and chemical engineers work together in industry. The astonishing developments in science and engineering during the 20th century have made it possible to dream of new goals that might previously have been considered unthinkable. This book identifies the key opportunities and challenges for the chemical sciences, from basic research to societal needs and from terrorism defense to environmental protection, and it looks at the ways in which chemists and chemical engineers can work together to contribute to an improved future.
