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Insect Cell Biotechnology

Insect Cell Biotechnology
Author: Gordon D. O. Maramorosch
Publisher: CRC Press
Total Pages: 458
Release: 2018-01-18
Genre: Science
ISBN: 135109047X

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Insect Cell Biotechnology provides a lucid, up-to-date description of recent major advances in the field. A number of significant topics are addressed, including the use and production of baculoviruses in insect cells, baculovirus specificity, bacterial toxin studies in cultured insect cells, scale-up operations required in the production of recombinant protein and insect viruses propagated in insect cells, growth and nervous system interactions, and the physiological and developmental capacities of cell lines. Transfection in Drosophila cells and a chapter on the theoretical and practical implications of stress produced by x-rays, ultraviolet light, chemicals, psoralens, and heat are discussed as well.


Insect Cell Culture Engineering

Insect Cell Culture Engineering
Author: Mattheus F. A. Goosen
Publisher: CRC Press
Total Pages: 260
Release: 2020-07-24
Genre: Science
ISBN: 1000105660

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Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.


Insect Cell Biotechnology

Insect Cell Biotechnology
Author: Karl Maramorosch
Publisher: CRC Press
Total Pages: 208
Release: 1994-05-27
Genre: Medical
ISBN:

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Insect Cell Biotechnology provides a lucid, up-to-date description of recent major advances in the field. A number of significant topics are addressed, including the use and production of baculoviruses in insect cells, baculovirus specificity, bacterial toxin studies in cultured insect cells, scale-up operations required in the production of recombinant protein and insect viruses propagated in insect cells, growth and nervous system interactions, and the physiological and developmental capacities of cell lines. Transfection in Drosophila cells and a chapter on the theoretical and practical implications of stress produced by x-rays, ultraviolet light, chemicals, psoralens, and heat are discussed as well.


Insect Cell Cultures

Insect Cell Cultures
Author: Just M. Vlak
Publisher: Springer Science & Business Media
Total Pages: 303
Release: 2006-04-11
Genre: Science
ISBN: 0306468506

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A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.


Insect Biotechnology

Insect Biotechnology
Author: Andreas Vilcinskas
Publisher: Springer Science & Business Media
Total Pages: 269
Release: 2010-10-27
Genre: Science
ISBN: 9048196418

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The book provides a fascinating overview about current and sophisticated developments in applied entomology that are powered by molecular biology and that can be summarized under a novel term: insect biotechnology. By analogy with the application of powerful molecular biological tools in medicine (red biotechnology), plant protection (green biotechnology) and industrial processing (white biotechnology), insect biotechnology (yellow biotechnology) provides novel tools and strategies for human welfare and nutrition. Insect Biotechnology has emerged as a prospering discipline with considerable economic potential, and encompasses the use of insect model organisms and insect-derived molecules in medical research as well as in modern plant protection measures.


Introduction to Insect Biotechnology

Introduction to Insect Biotechnology
Author: Dhiraj Kumar
Publisher: Springer Nature
Total Pages: 144
Release: 2023-06-23
Genre: Science
ISBN: 3031267761

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This textbook introduces to the fundamentals and applications of insect biotechnology in a concise manner. Global economically important insects such as silkworm, lac or honey bee are in the focus of this text. The book attempts to address all of the latest developments in this growing field such as application of metagenomics and proteomics in mining for genes and enzymes that could be beneficial in diverse industrial and biomedical applications. Further, insects have been established as suitable tool for the production of transgenic products which is discussed in one dedicated chapter. The book targets students and researchers in molecular biology, biotechnology or entomology who want to get familiar with the emerging field of insect biotechnology.


Yellow Biotechnology II

Yellow Biotechnology II
Author: Andreas Vilcinskas
Publisher: Springer
Total Pages: 215
Release: 2014-01-10
Genre: Science
ISBN: 3642399029

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Insect derived enzymes – a treasure for white biotechnology and food biotechnology. Insect-derived chitinases. Cellulases from insects. Optimization of Insect Cell Based Protein Production Processes - Expression Systems, Online Monitoring, Scale-Up. Insect antenna-based biosensors for in situ detection of volatiles. Y-linked markers for improved population control of the tephritid fruit fly pest, Anastrepha suspensa. Transgenic Approaches to Western Corn Rootworm Control. Tribolium castaneum as a model for high-throughput RNAi screening. Aphid-proof plants: Biotechnology-based approaches for aphid control.


Baculovirus and Insect Cell Expression Protocols

Baculovirus and Insect Cell Expression Protocols
Author: David W. Murhammer
Publisher: Methods in Molecular Biology
Total Pages: 522
Release: 2018-04-15
Genre: Medical
ISBN: 9781493979783

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The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.


Gene Expression Systems

Gene Expression Systems
Author: Joseph M. Fernandez
Publisher: Elsevier
Total Pages: 493
Release: 1998-12-21
Genre: Science
ISBN: 0080532357

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Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method


Trends in Insect Molecular Biology and Biotechnology

Trends in Insect Molecular Biology and Biotechnology
Author: Dhiraj Kumar
Publisher: Springer
Total Pages: 378
Release: 2018-04-03
Genre: Science
ISBN: 331961343X

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This book provides an overview on the basics in insect molecular biology and presents the most recent developments in several fields such as insect genomics and proteomics, insect pathology and applications of insect derived compounds in modern research. The book aims to provide a common platform for the molecular entomologist to stimulate further research in insect molecular biology and biotechnology. Insects are one of the most versatile groups of the animal kingdom. Due to their large population sizes and adaptability since long they attract researchers’ interest as efficient resource for agricultural and biotechnological purposes. Several economically important insects such as Silkworms, Honey Bee, Lac and Drosophila or Termites were established as invertebrate model organisms. Starting with the era of genetic engineering, a broad range of molecular and genetic tools have been developed to study the molecular biology of these insects in detail and thus opened up a new horizon for multidisciplinary research. Nowadays, insect derived products are widely used in biomedical and biotechnology industries. The book targets researchers from both academia and industry, professors and graduate students working in molecular biology, biotechnology and entomology.